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Preparing technique of nano magnetic gene-loaded liposome of temperature-controlled release

A nano-magnetic and liposome technology, which is applied in liposome delivery, gene therapy, medical preparations of non-active ingredients, etc., can solve the problem of single function, achieve uniform size, good heating ability, and high stability Effect

Inactive Publication Date: 2010-06-23
SOUTHEAST UNIV
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Problems solved by technology

[0004] The prospect of magnetic nanomaterial hyperthermia is very broad, but if pure hyperthermia is not combined with other treatment methods, its role is still single

Method used

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  • Preparing technique of nano magnetic gene-loaded liposome of temperature-controlled release
  • Preparing technique of nano magnetic gene-loaded liposome of temperature-controlled release
  • Preparing technique of nano magnetic gene-loaded liposome of temperature-controlled release

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Embodiment

[0025] Example A preparation method of nano-magnetic gene-carrying heat-controlled release liposome as a drug for treating cancer:

[0026] The first step: manganese sulfate MnSO 4 ·H 2 O, zinc sulfate ZnSO 4 ·7H 2 O, ferrous sulfate FeSO 4 ·7H 2 O is mixed in molar ratio 1: 1: 4 to obtain the composition, placed in an electric pulverizer and pulverized into powder, then weighed sodium hydroxide according to the metal ion in the composition and sodium hydroxide NaOH molar ratio of 1: 2.4 and used Dissolve in distilled water, then add aqueous sodium hydroxide solution to the powder and stir for three hours with an electric mixer, let it stand for 12 hours, then dry it in an oven at 80°C, then bake it in a muffle furnace at 400°C for 1 hour, grind the material finely after cooling into powder, soaked with 50-60°C distilled water for 6-8 times to remove soluble sodium sulfate inorganic salts, then rinsed with absolute ethanol, and dried at 60°C to obtain manganese-zinc ferro...

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Abstract

A preparing technique of a nano magnetic gene-loaded liposome of temperature-controlled release relates to a medicament for treating cancers. Manganese sulfate, zinc sulphate and ferrous sulphate are crushed into power according to a certain ratio and dissolved by sodium hydroxide, and nano magnetic particles of manganese, zinc and ferrum are obtained after the steps of agitation, drying, baking, etc. The nano magnetic particles of manganese, zinc and ferrum are decorated with a proper amount of polyethyleneimine, and then combined with expression plasmids of green florescent protein, so the nano magnetic gene-loaded particles of manganese, zinc and ferrum are obtained. A proper amount of dipalmitoyl phosphatidyl choline and cholesterol is added to an eggplant-shaped flask and dissolved by chloroform absolute ether. The eggplant-shaped flask is subsequently put in a rotating evaporator for evaporation, and a uniform film is formed on the bottom of the flask as a result. Then, phosphate buffer containing the nano magnetic gene-loaded particles of manganese, zinc and ferrum is added and electrically agitated until the film falls off. Glycerol is added for repeated freeze-thaw, and the nano magnetic gene-loaded liposome of temperature-controlled release is obtained.

Description

technical field [0001] The invention relates to a preparation process of a nano-magnetic gene-carrying heat-controlled release liposome as a medicine for treating cancer. Background technique [0002] The invention relates to a preparation process of a new type of nano-magnetic gene-carrying heat-controlled release liposome, which is prepared by an improved film dispersion and high-pressure homogenization method to prepare a kind of encapsulated anti-tumor gene and manganese-zinc ferromagnetic nanoparticles with The new type of nano heat-controlled release liposome with suitable phase transition temperature (41°C-46°C), because the encapsulated manganese-zinc-ferromagnetic nanoparticles have the characteristics of magnetic induction temperature rise, temperature control, and constant temperature in the alternating magnetic field, so when the temperature rises When the phase transition temperature of the liposome is reached, the gene can be released slowly, realizing gene the...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K48/00A61K47/28A61K47/42A61K47/32A61K47/04A61P35/00
Inventor 张东生王丽王子妤张佳刘静
Owner SOUTHEAST UNIV
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