Asia I synthetic peptide vaccine of foot and mouth disease

Abstract

The invention provides an Asia I synthetic peptide vaccine of a foot and mouth disease, in particular to a polypeptide or a polypeptide polymer thereof for the Asia I synthetic peptide vaccine of the foot and mouth disease, the vaccine containing the polypeptide or the polypeptide polymer thereof, and a preparation method of both, wherein, the amino acid sequence of the polypeptide is shown as SEQ ID NO:1. In the invention, variation situation of main antigen sites of the foot and mouth disease is researched by determining a sequence of an epidemic strain of the foot and mouth disease, and meanwhile analysis prediction of the antigen sites of the foot and mouth disease is performed followed by chemical synthesis on possible peptide segments of the antigen sites combined with a computer auxiliary method; and candidate polypeptide antigens are screened through a large quantity of animal experiments to finally obtain the polypeptide antigens with high immune reaction level which can protect well an animal from being attacked by the epidemic strain of the foot and mouth disease. The vaccine prepared from the polypeptide antigens has the advantages of no biological safety problem, easylarge-scale synthesis, good application prospect and good ability of effectively dealing with antigen variation of foot and mouth disease virus.

Description

technical field [0001] The present invention relates to a polypeptide or its polypeptide polymer used for foot-and-mouth disease synthetic peptide vaccines, vaccines containing the polypeptide or its polypeptide polymer and their preparation methods; in particular to a synthetic peptide vaccine for Asian type I foot-and-mouth disease Polypeptide or its polypeptide polymer, vaccines containing the polypeptide or its polypeptide polymer and their preparation methods. Background technique [0002] Foot and mouth disease (FMD) is an acute, highly contagious, febrile infectious disease that occurs in cloven-hoofed animals. Foot-and-mouth disease is caused by foot-and-mouth disease virus, which belongs to the genus of foot-and-mouth disease virus in the family Picornaviridae. It is spherical in shape with a diameter of 30nm and no envelope. Foot-and-mouth disease virus has a simple structure and is composed of single-stranded RNA and protein. It is most abundant in the blister sk...

AI Technical Summary

Problems solved by technology

However, due to the limited amount of gene expression products and the inability to obtain the three-dimensional structure of the antigen, it is difficult to improve the immune efficacy

In 2003, Zheng Zhaoxin et al. concatenated the T-cell epitope and B-cell epitope genes on VP1 and VP4 to develop a genetically engineered vaccine against Asia 1 type foot-and-mouth disease, but the test results showed that it could not reach the target level under virulent attack. 100% protection, at the same time as a vaccine, the post-processing of genetically engineered vaccines is too complicated, the cost is high, and the potency is low

At present, the Asian type 1 FMD virus is one of the main FMD viruses prevalent in my country. The antigenicity of the FMD strain is still changing. As time goes by, the effectiveness of the original vaccine weakens or even disappears. The prevention and control work has brought great difficulties

Moreover, the degree of antigenic variation within the same serotype is so great that an FMD vaccine effective against one subtype may not be protective against another subtype within the same serotype

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