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Zooblast cultivation method

An animal cell culture and animal cell technology, applied in the field of animal cell culture, can solve problems such as poor safety, achieve low cost, promote animal cell growth, and achieve small differences.

Inactive Publication Date: 2009-03-18
BEIJING SKYWING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the need to use high-content animal serum (such as animal serum with a final concentration of at least 10% by volume) in the existing animal cell culture method to promote animal cell growth and poor safety, and to provide a new animal cell culture method. A cell culture method, which can promote the growth of animal cells with a low concentration of animal serum and has good safety

Method used

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  • Zooblast cultivation method
  • Zooblast cultivation method
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Examples

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preparation example Construction

[0025] Preparation method of dry animal cell culture medium powder The composition of dry animal cell culture medium powder of the above medium components is dispersed in a dispersing agent, and the dispersing agent is removed from the resulting mixture. The dispersion of the components of the animal cell culture medium dry powder composition in the dispersant can be achieved by various dispersion methods commonly used in the art, such as stirring, ultrasonic oscillation and the like.

[0026] Two types of methods, dry method and wet method, can be used to prepare the animal cell culture medium dry powder composition used in the present invention. The so-called dry method refers to mixing powders of solid components under dry conditions (such as using a ball mill for ball milling and mixing). When using the dry method, it is preferable to dry the components of the dry medium powder composition firstly according to their properties. For example, anhydrous sodium dihydrogen pho...

Embodiment 1

[0039] This example is used to illustrate the animal cell culture method of the present invention.

[0040] table 3

[0041] components mg / L components mg / L L-alanine 90 Calcium Nitrate Tetrahydrate 50 L-arginine hydrochloride 200 potassium chloride 400 L-Aspartic Acid 20 Anhydrous Magnesium Sulfate 43 L-Asparagine 30 Sodium chloride 6400 L-cysteine ​​hydrochloride 20 Anhydrous Disodium Hydrogen Phosphate 320 L-glutamic acid 130 Ferrous Sulfate Heptahydrate 0.2 L-Glutamine 300 Zinc sulfate heptahydrate 0.3 L-histidine hydrochloride 30 D-glucose 2000 L-Hydroxyproline 10 Glutathione 0.3 L-isoleucine 120 sodium pyruvate 50 L-leucine 30 ascorbic acid 3 L-Lysine Hydrochloride 150 Choline chloride 15 L-Methionine 20 Calcium D-pantothenate 3 L-phenylalanine 30 folic acid 5 L-proline 40 Nicotinamide 5 L-serine 30 Pyridoxine hydrochl...

Embodiment 2

[0046] This example is used to illustrate the animal cell culture method of the present invention.

[0047] Table 4

[0048] components mg / L components mg / L L-alanine 110 Calcium Nitrate Tetrahydrate 60 L-arginine hydrochloride 190 potassium chloride 320 L-Aspartic Acid 10 Anhydrous Magnesium Sulfate 60 L-Asparagine 50 Sodium chloride 7300 L-cysteine ​​hydrochloride 10 Anhydrous Disodium Hydrogen Phosphate 380 L-glutamic acid 100 Ferrous Sulfate Heptahydrate 0.4 L-Glutamine 500 Zinc sulfate heptahydrate 0.8 L-histidine hydrochloride 45 D-glucose 3000 L-Hydroxyproline 5 Glutathione 0.9 L-isoleucine 90 sodium pyruvate 60 L-leucine 70 ascorbic acid 7 L-Lysine Hydrochloride 100 Choline chloride 10 L-Methionine 40 Calcium D-pantothenate 4 L-phenylalanine 50 folic acid 3 L-proline 60 Nicotinamide 4 L-serine 60 Pyridoxine hydrochlo...

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Abstract

The invention provides a method for culturing an animal cell. The method does not need to introduce extra protein component outside animal serum (such as recombinant protein, vegetable protein or animal source component like cytokine); because using low content animal serum can achieve the same or even better effect as using high content animal serum in the aspect of promoting growth of the animal cell, the method for culturing the animal cell does not produce side effects accompanied by the protein component outside the animal serum, and has the advantages of good security and low cost. In addition, the method for culturing the animal cell uses less animal serum, therefore cells cultured by the method have little difference between different batches, low cost, good security, are good for separating cell downstream products, and are suitable to be virus hosts, expression vectors of biological products like vaccines.

Description

technical field [0001] The invention relates to a method for culturing cells, in particular to a method for cultivating animal cells. Background technique [0002] In the existing animal cell culture methods, it is generally necessary to use animal serum with a final concentration of at least 10% by volume in order to ensure the normal growth and passage of cells. [0003] Animal serum, however, is a complex mixture of ill-defined components. On the one hand, animal serum contains, for example, transport proteins carrying hormones, minerals, trace elements, and lipids; cell adhesion factors and diffusion factors can promote cell growth, stabilize detoxification, maintain the pH value of the medium, and inhibit proteases from directly or indirect enzymatic hydrolysis. But on the other hand, there are many problems with animal serum. For example, the pH value of the medium changes greatly during the process of adding and supplementing, and it is easily contaminated by other ...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/07
Inventor 张韧陈文庆
Owner BEIJING SKYWING TECH CO LTD
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