Gene chip and reagent box for detecting food-borne virus

Abstract

The invention relates to a gene chip for detecting food-home virus and a kit, belonging to the inspection field. The surface of a solid phase carrier is fixed with a plurality of detection probes and quality control contrast probes, wherein, the detection probes consist of the probes for detecting hepatitis A virus, human astrovirus, norwalk virus G1, norwalk virus G2, rotavirus, polio virus 1, polio virus 2 and polio virus 3; and the quality control contrast probes consist of a spotting positive quality control probe, a chip hybridization positive quality control probe and a chip negative quality control probe. The gene chip and the kit have the advantages of: (1) high throughout: five common viruses are integrated and detected simultaneously, and the practicability is strong; (2) rapidness: the detection time is only 4 hours; (3) specificity: the false positive caused by the cross reaction is avoided; (4) flexibility: the detection flexibility of the chip is 10<8> virus particles per gram of the tissue sample, which is higher than that of RT-PCR; and (5) good repetitiveness and stable results. The gene chip and the kit can be widely applied to the food safety inspection of the inspections system.

Description

technical field [0001] The invention relates to a gene chip and a kit for detecting food-borne viruses, belonging to the technical field of inspection and quarantine. Background technique [0002] Food safety has always been a hot issue of concern to the international community and governments of various countries. It is estimated that 76,000,000 people suffer from food poisoning and 5,000 people die every year in the United States. At present, more than 200 kinds of diseases are known to be transmitted through food, and more than half of the food poisoning cases have not found the cause, indicating that there are more than 200 causes of food poisoning. Among food microbial poisoning factors, due to the limitation of detection methods, usually only bacterial indicators are detected, and virus contamination in food cannot be effectively monitored and analyzed. According to the US FDA estimates, food poisoning caused by viruses leads to Nearly 10,000,000 cases, 128 deaths; No...

AI Technical Summary

Problems solved by technology

[0004] It is very difficult to culture and isolate food-borne viruses in vitro. For example, among the most common and important Norwalk virus and hepatitis A virus in food poisoning, only hepatitis A virus can reproduce in host cells and use host cells for replication. The virus grows slowly in cells, and it takes 3-4 generations of culture to show visible lesions, and the virus content in food is often very low. Therefore, it often takes 30-60 days to isolate hepatitis A virus by the culture method, which is not suitable for use in Detection of hepatitis A virus in food

Norwalk virus can not reproduce in vitro, and there is no animal model, and it cannot be isolated and detected by tissue culture or animal experiments. At the same time, its infectious dose is very low, estimated to be 10 0 -10 2 Infectious units, the detection limit of conventional electron microscopy is 10 5 -10 6 Virus particles / ml, the sensitivity cannot meet the requirements to ensure food safety

Recently, radioimmunoassay (EIA) based on ELISA technology has been used for the clinical diagnosis of Norovirus, but it is not suitable for the detection of viruses in food due to the limitation of the detection limit.

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