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Zedoary turmeric oil analysis method

An analytical method and technology of turmeric oil, applied in the field of analysis of turmeric oil and its preparations, can solve problems such as poor specificity, and achieve the effects of improving accuracy and improving separation.

Inactive Publication Date: 2008-03-19
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the vanillin sulfuric acid chromogenic agent is a general-purpose chromogenic agent with poor specificity, it has been proved by experiments that the zedoary oil injection that does not contain curcumol will show a false positive reaction when measured by this method, and the content is often higher than 68%, indicating that this method Not suitable as a method for determination of zedoary oil injection

Method used

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  • Zedoary turmeric oil analysis method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: select reversed-phase HPLC method to qualitatively and quantitatively analyze 6 kinds of sesquiterpenoid reference substances,

[0012] Instrument: American Agilent1100 high performance liquid chromatography: equipped with DE23922775 quaternary pump; DE2392205series automatic sampling system; JP24020624 ultraviolet detector; JP13208646 online degasser; Agilent Rev.A.09.03[1417] chemical workstation.

[0013] Chromatographic conditions: Discovery C18HPLC column: 25cm×4.6mm×5um; mobile phase: methanol-water (90:10);

[0014] Detection wavelength: 215nm; column temperature: room temperature; flow rate: 1ml / min; injection volume: 20μl.

[0015] Control substance:

[0016] Gemarone (geranone) was provided by the Heilongjiang Provincial Drug Inspection Institute;

[0017] Curcumol was provided by China National Institute for the Control of Pharmaceutical and Biological Products;

[0018] β-Elemene was provided by Dalian Institute of Pharmaceutical Sciences; ...

Embodiment 2

[0020] Embodiment 2: Select reversed-phase HPLC method to qualitatively and quantitatively analyze supercritical CO 2 Curcuma Oil Prepared by Fluid Extraction

[0021] Instrument: Same as Example 1;

[0022] Chromatographic conditions: Discovery C 18 HPLC column: 25cm×4.6mm×5um; mobile phase: methanol-water (90:10);

[0023] Detection wavelength: 215nm; column temperature: room temperature; flow rate: 1ml / min; injection volume: 20μl.

[0024] Test article: supercritical CO 2 Fluid extraction process preparation (curcuma medicinal materials were purchased from Wenzezhu Professional Cooperative in Shazhou Village, Taoshan Town, Ruian City, and was identified as the dry rhizome of Curcuma wenyujinY.H.Chen et C.Ling by Professor Bao Wenfang, School of Chinese Medicine, Shenyang Pharmaceutical University. Extraction process : the extraction pressure is 18Mpa, and the extraction temperature is 55°C; the separation kettle I pressure is 12Mpa, and the temperature is 55°C, the sepa...

Embodiment 3

[0026] Embodiment 3: select reversed-phase HPLC method to qualitatively and quantitatively analyze the zedoary oil-1 prepared by steam distillation

[0027] Instrument: Same as Example 1;

[0028] Chromatographic conditions: Discovery C 18 HPLC column: 25cm×4.6mm×5um; mobile phase: methanol-water (90:10);

[0029] Detection wavelength: 215nm; column temperature: room temperature; flow rate: 1ml / min; injection volume: 20μl.

[0030] Test product: Zedoary oil-1 obtained by steam distillation (the source of the medicinal material of Zedoary is the same as in Example 2, obtained according to the extraction process under the item of Zedoary Oil in Part 1 of "Chinese Pharmacopoeia" 2005 edition), dissolved in methanol, and the concentration is 35.6 μg / ml solution. The test results show that the zedoary oil contains 18.6% of furandadiene, 15.3% of curcumene, 10.4% of gemaconone and 6.7% of curdione (see accompanying drawing 3).

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Abstract

The present invention discloses a HPLC analysis method of zedoary turmeric oil and the preparation thereof. The HPLC analysis method of the present invention can be used in the assay determination on separating the compositions of the zedoary turmeric oil extracted with various preparation techniques (supercritical CO2 liquid extraction, vapor distillation, organic solvent extraction, and so on); can be used in the assay determination on separating the compositions of the zedoary turmeric oil in various preparations made of the zedoary turmeric oil; can be used in the monitoring during the zedoary turmeric oil preparing process and the monitoring of the middle sample of the zedoary turmeric oil preparation; and can be used in the examination of the matters concerned in the zedoary turmeric oil and the study of the preparation fingerprint spectrum. The HPLC analysis method of the present invention is simple and quick, has high sensitivity and excellent repeatability, and can reflect the main compositions of the zedoary turmeric oil exactly and really.

Description

technical field [0001] The invention relates to an analysis method of zedoary oil and a preparation thereof, in particular to an HPLC analysis method of the zedoary oil and a preparation thereof. Background technique [0002] Zedoary oil and its injection are listed in the Chinese Pharmacopoeia, and are clinically proven antiviral and anticancer drugs. The separation and analysis method of Zedoary Oil has always been gas chromatography (GC) or gas chromatography-mass spectrometry (GC-MS), but because the effective components of Zedoary Oil are various sesquiterpenoids, and GC or GC The high temperature of MS injection chamber and chromatographic column makes some sesquiterpenoids transform under high heat conditions, which affects the clinical efficacy of zedoary oil and the stability of its preparations. For example, the anti-cancer main component of warm zedoary oil-furanodiene (Furanodiene, also known as: zedoary cyclodiene, molecular formula: C 15 h 20 O) During the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 孙秀燕
Owner YANTAI UNIV
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