The invention discloses a
color film substrate, a manufacturing method of the
color film substrate and a display panel, the
color film substrate is provided with a display area and a non-display areasurrounding the display area, and the non-display area is defined to comprise a first non-display area right opposite to a double-layer
metal wiring area of an array substrate and a second non-displayarea right opposite to a single-layer
metal wiring area of the array substrate. The color film substrate further comprises: a substrate body; a
black matrix layer which is arranged on one surface ofthe substrate body, wherein a first receding groove is formed in the surface, deviating from the substrate body, of the
black matrix layer, and the first receding groove is located in the first non-display area; and a conducting layer which is arranged on the side, deviating from the substrate, of the
black matrix layer, wherein at least part of the conducting layer is sunken in the first recedinggroove to form a second receding groove. According to the technical scheme, when the particle size of the gap supporting ball is calculated according to the longitudinal sections of the first non-display area and the second non-display area, the particle size of the gap supporting ball is uniform, and then it is guaranteed that the box thickness of the display panel is uniform.