31 results about "Angiogenic Peptides" patented technology

Anti-angiogenic peptides that inhibit activation or proliferation of endothelial cells are disclosed. Such peptides maybe used to inhibit VEGF binding to the VEGFR2 receptor (also known as the kinase domain receptor or KDR) and bFGF binding to its receptor. Such peptides may also be used to inhibit, VEGF, bFGF, or integrin activation of endothelial cells in angiogenesis-associated diseases such as cancer, leukemia, multiple myeloma, inflammatory diseases, eye diseases and skin disorders.

Compositions and methods that are useful for modulating blood vessel formation, as well as methods that provide for the systematic and efficient identification of angiogenesis modulators are described. As discussed in more detail below, a systematic computational methodology based on bioinformatics was used to identify novel peptide modulators of angiogenesis that have been characterized in vitro and / or in vivo.

The invention generally features compositions and methods that are useful for modulating blood vessel formation, as well as methods that provide for the systematic and efficient identification of angiogenesis modulators. As described in more detail below, a systematic computational methodology based on bioinformatics was used to identify novel peptide modulators of angiogenesis that have been characterized in vitro and / or in vivo.

Modified anti-angiogenic peptides are disclosed. The modified peptides are capable of forming a peptidase stabilized anti-angiogenic peptide. The modified anti-angiogenic peptides, particularly modified kringle 5 peptides are capable of forming a conjugate with a blood protein. Conjugates are prepared from anti-angiogenic peptides, particularly kringle 5 peptides, by combining the peptide with a reactive functional group with a blood protein. The conjugates may be formed in vivo or ex vivo. The conjugates are administered to patients to provide an anti-angiogenic effect.

Short bioactive sequences derived from the 2nd loop of the 7-transmembranal receptor of endothelial differentiation gene 3 (EDG3) useful in stimulation of angiogenesis, and peptide conjugates comprising a permeability enhancing moiety, are provided. Also provided are pharmaceutical compositions comprising the peptides and methods of use in conditions were insufficient blood-supply occurs, or which are associated with endothelia dysfunction such as peripheral vascular diseases, coronary artery diseases, cerebrovascular diseases, diabetes and delayed wound healing, pulmonary disease, eye diseases and pathological condition related to severe infection.

A peptide comprising an amino acid sequence as set forth in SEQ ID NO: 2, 4, 6, 8, 10 or 12 is provided. The peptide being at least 6 and no more than 50 amino acid residues in length. Also provided are therapeutic applications using such peptides.

The invention relates to a bioactive peptide prediction method based on a deep convolutional neural network, which processes an original amino acid residue sequence through one-dimensional convolution of filters with different sizes, extracts useful features, optimizes a model through optimization model output and label cross entropy loss, and is specially designed for AAP mining and prediction. The model, namely the AAPred-CNN is based on an embedding technology but not based on features of feature engineering and manual design, data sets used for training and testing of the AAPred-CNN are a disclosed data set main and an NT15 data set, the classic deep learning algorithm TextCNN is combined with the embedding technology, residue tendency analysis and the like are applied to the prediction problem of the anti-angiogenic peptide for the first time, and the prediction accuracy of the anti-angiogenic peptide is improved. A classifier AAPred-CNN with excellent performance is designed for mining and predicting the AAP, and the AAPred-CNN is based on an embedding technology, does not depend on feature engineering, and can extract useful information from a pure amino acid residue sequence in a self-adaptive mode and is used for predicting whether polypeptide has anti-angiogenesis functional activity or not.

The present invention refers to a pharmaceutical composition comprising an isolated antiangiogenic peptide or a recombinant protein comprising the antiangiogenic peptide, wherein the peptide is between 11 and 40 amino acids in length and having antiangiogenic activity, the peptide comprising the amino acid sequence: X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14, wherein X1 is any amino acid residue compatible with forming a helix; X2 is an amino acid residue of: Leu, Ile, Val; X3 is an amino acid residue of: Arg, Lys, His, Ser, Thr; X4 is an amino acid residue of: Ile, Leu, Val; X5 is any amino acid residue compatible with forming a helix; X6 is an amino acid residue of: Leu, Ile, Val; X7 is an amino acid residue of: Leu, Ile, Val, Ser, Thr; X8 is any amino acid residue compatible with forming a helix; X9 is any amino acid residue compatible with forming a helix; X10 is an amino acid residue of: Gln, Glu, Asp, Arg, His, Lys, Asn; X11 is an amino acid residue of: Ser, Thr; X12 is an amino acid residue of: Trp, Tyr, Phe; X13 is an amino acid residue of: Leu, Ile, Val, Asn, Gln; X14 is an amino acid residue of: Glu, Gln, Asp, Asn.

This present invention is directed to peptides, compositions, and methods for modulating endogenous cytokine expression in a subject. More specifically, the invention provides peptides useful in regulating the release of a specific pattern of cytokines that promote angiogenesis and / or can be used to modulate the immune system of a subject.

The present invention provides microparticle compositions comprising antiangiogenic peptides, as well as methods of treatment, including for macular degeneration. In various aspects and embodiments, the present invention provides microparticle compositions providing extended release of anti-angiogenic peptides. The microparticle of the present invention comprises poly(lactide-co-glycolide) (PLGA)having a having lactic acid (LA) to glycolic acid (GA) ratio (LVG) of more than 1:1; the microparticle further comprises, e.g., encapsulates, an anti-angiogenic peptide derived from the alpha 5 fibrilof type IV collagen.

This present invention is directed to peptides, compositions, and methods for modulating endogenous cytokine expression in a subject. More specifically, the invention provides peptides useful in regulating the release of a specific pattern of cytokines that promote angiogenesis and / or can be used to modulate the immune system of a subject.

The invention belongs to the technical field of tumor therapies, and particularly relates to an FAP-targeted anti-angiogenesis peptide Z-GP-V2 with an anti-tumor angiogenesis effect. The molecular weight of the synthetic peptide is 1149.6, and the sequence of the synthetic peptide is Z-GPAANLLMAAS; the synthetic peptide is prepared by adopting an Fmoc solid-phase polypeptide synthesis method and used for preparing an anti-tumor angiogenesis preparation. Accordingly, the novel synthetic peptide Z-GP-V2 is obtained by modifying a V2 peptide through a specific substrate dipeptide Z-GP, and the novel synthetic peptide has the good application effects on improvement of the targeting property and the anti-tumor angiogenesis effect of the V2 peptide, reduction of the toxic and side effects of the V2 peptide and the like and provides new use for reference for a novel anti-tumor angiogenesis therapeutic method.

Short bioactive sequences derived from the 2nd loop of the 7-transmembranal receptor of endothelial differentiation gene 3 (EDG3) useful in stimulation of angiogenesis, and peptide conjugates comprising a permeability enhancing moiety, are provided. Also provided are pharmaceutical compositions comprising the peptides and methods of use in conditions were insufficient blood-supply occurs, or which are associated with endothelia dysfunction such as peripheral vascular diseases, coronary artery diseases, cerebrovascular diseases, diabetes and delayed wound healing, pulmonary disease, eye diseases and pathological condition related to severe infection.

The invention belongs to the field of nano-drugs, and relates to an esterase response nano-drug as well as a preparation method and application thereof. The amphipathic molecules are prepared by forming an ester bond by carboxyl at the C terminal of a polypeptide molecule and hydroxyl of a drug. The polypeptide molecules are annular anti-angiogenesis peptide VGB, and the drug is oridonin ORI. The amphiphilic molecules can be self-assembled into a nano-drug in a water-phase solution under a neutral condition. The nano-drug can release VGB and ORI at a tumor part through enzyme specific enzymolysis. The nano-drug provided by the invention has the advantages of simple preparation method, no covalent bond generation in the self-assembly process, no reverse reaction, good biocompatibility, low toxic and side effects and esterase responsiveness, and has high application value and wide application prospect in preparation of nano-drugs applied to tumor resistance.

The present invention provides an anti-angiogenic peptide comprising an amino acid sequence having at least 70% identity to amino acid residues 123-140 of SEQ ID NO 1 or amino acid residues 24-141 of SEQ ID NO 2. The invention also provides nucleic acid constructs encoding such peptides, and vectors and cells comprising such nucleic acid constructs. The invention further provides pharmaceutical compositions comprising the peptides or nucleic acid constructs of the invention, and the use of peptides, nucleic acid constructs or pharmaceutical compositions of the invention to treat diseases associated with angiogenesis.10

The present application discloses angiogenic peptides that cause intracellular calcium release in target cells and thereby induce proliferation, migration, and capillary-like tube formation in primary cultured endothelial cells. The angiogenic peptides can be used for preventing and / or treating angiognesis-related conditions, especially wound healing, treating foot and leg ulcers in a subject, etc. In addition, the angiogenic peptides can be used for cosmetics a constituent of cosmetics for aged skin, for examples, anti-wrinkle and skin whitening.

The present application discloses angiogenic peptides that cause intracellular calcium release in target cells and thereby induce proliferation, migration, and capillary-like tube formation in primary cultured endothelial cells. The angiogenic peptides can be used for preventing and / or treating angiognesis-related conditions, especially wound healing, treating foot and leg ulcers in a subject, etc. In addition, the angiogenic peptides can be used for cosmetics a constituent of cosmetics for aged skin, for examples, anti-wrinkle and skin whitening.

The present invention relates to methods and pharmaceutical compositions for the treatment of tissue lesions. The inventors showed that CCR2 is expressed on FMCs, especially on a subpopulation of progenitor cells, that they call “fetal myeloid progenitor cells” (FMPCs), and mediates the recruitment of these cells to maternal wound tissue. Moreover, the inventors reported that recruited FMCs / FMPCs improve maternal skin wound healing by organizing blood vessel endothelium and secreting pro-angiogenesis peptides, particularly chemokine CXCL1, to enhance angiogenesis in wound. In particular, the present invention relates to CCR2 agonists for use in the treatment of tissue lesions in a subject in need thereof.