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Angiogenic peptide

一种血管生成、血管发生的技术，应用在血管生成肽领域，能够解决血管问题等问题

Active Publication Date: 2011-05-18
NOVACELL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interference with the angiogenic switch can cause serious blood vessel problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Materials and methods for the characterization of FPRL1 antagonistic peptides

[0072] 1.1. Materials

[0073] Fura-2 pentaacetoxymethylester (pentaacetoxymethylester) (Fura-2-AM) and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetoxymethyl Ester (BAPTA-AM) was purchased from Molecular Probes (Eugene, OR). Pertussis toxin (PTX), U73122 and U73433 were from Calbiochem (San Diego, CA). Matrigel was from Becton Dickinson (Bedford, MA). Recombinant human VEGF and anti-VEGF neutralizing antibody were from R&D systems (Minneapolis, MN). Peptides were synthesized by Peptron Inc. (Korea). PS-SPCL at the Peptide Library Support Facility of Pohang University of Science and Technology (Korea) as previously described (S.H.Baek, et al, J Biol Chem 271 (1996), pp.8170-8175; R.A.Houghten, et al., Nature 354 (1991), pp.84-86) to prepare.

[0074] 1.2. Cell culture

[0075] At 37°C, in a supply of 95% air and 5% CO 2 MS-1 cells and B16F1 murine melanoma cells were cultured in ...

Embodiment 2

[0093] 2.1. Identification of induced [Ca in MS-1 cells 2+ ] i elevated peptide

[0094] To identify peptides that stimulate intracellular calcium mobilization in murine endothelial cells (MS-1 cells), the inventors screened a library of 114 C-terminally amidated synthetic hexapeptides. The results of the initial screening of the peptide library in MS-1 cells are shown in Figures 1A-1F .

[0095] Figures 1A-1F Initial PS-SPCL screening for peptides that elevate intracellular calcium in MS-1 cells is shown. Each panel shows the results obtained for a peptide library with known amino acids at each of the six positions of the hexapeptide. The 6 positions are each defined by one of the 19 L-amino acids (eg O1, O2). The remaining 5 positions consist of the mixture (X) of the 19 L-amino acids (excluding cysteine). The library consisted of 114 peptide pools; PS-SPCL consisted of 47,045,881 different peptides in total. As described in Example 1, [Ca 2+ ] i raised. The resul...

Embodiment 3

[0131] Example 3: Promotion of Wound Healing in Animal Models

[0132]Sprague-Dawley (6 weeks old, male, body weight 140-160 g) was used for wound healing experiment, and the animals were randomly divided into 3 groups. Under gerolan anesthesia, the back was depilated and the skin was sterilized with 70% ethanol. A full-thickness wound was made on the dorsal skin using an 8-mm skin biopsy punch. The wound site was covered with Tegaderm. The present inventors topically administered control (HBSS), SFKLRY 10 uM and fysrlk 10 uM to each group once a day for 14 days. After 14 days, the rats were sacrificed and the wounded tissue was removed. These samples were then individually fixed in 4% formaldehyde, dehydrated through a graded ethanol series, cleared in xylene and embedded in paraffin. Serial sections of 4 μm were cut and stained with hematoxylin and eosin (H&E).

[0133] Based on the positive effect of SFKLRY-NH2 (SEQ ID NO: 2) on HUVEC migration in an in vitro wound mig...

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Abstract

The present application discloses angiogenic peptides that cause intracellular calcium release in target cells and thereby induce proliferation, migration, and capillary-like tube formation in primary cultured endothelial cells. The angiogenic peptides can be used for preventing and / or treating angiognesis-related conditions, especially wound healing, treating foot and leg ulcers in a subject, etc. In addition, the angiogenic peptides can be used for cosmetics a constituent of cosmetics for aged skin, for examples, anti-wrinkle and skin whitening.

Description

technical field [0001] The present invention relates to angiogenic peptides that cause intracellular calcium release in target cells and thereby induce proliferation, migration and capillary-like tube formation of primary cultured endothelial cells. In addition, the angiogenic peptides can be used for the prevention and / or treatment of angiogenesis-related disorders, especially wound healing, treatment of foot and leg ulcers in subjects, and the like. In addition, the angiogenic peptides can be used in cosmetics, as a component of cosmetics against skin aging, for example for anti-wrinkle and skin whitening. Background technique [0002] Angiogenesis—the growth of new blood vessels from existing vasculature—is an essential process in a variety of physiological and pathological conditions, including wound healing, embryonic development, chronic inflammation, and tumor progression and metastasis (J. Folkman, Nat. Med. 1 (1995), pp. 27-31; W. Risau, Nature 386 (1997), pp. 671-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08C07K14/515A61K38/08A61K38/10A61K8/64A61P17/00A61Q19/00
CPCC07K7/06A61K8/64A61Q19/08A61K38/00A61Q19/02A61P9/00A61P17/00A61P17/02A61P43/00
Inventor 李泰勋
Owner NOVACELL TECH
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