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Application of cumarin kind compound in preparation of medicine for inducing nerve stem cell orienting differentiation

A neural stem cell and coumarin technology, which is applied in the application field of coumarin compounds in the preparation of drugs for inducing neural stem cell directional differentiation, can solve the problem of staying, unable to be used in clinic, and unable to obtain a large number of oligodendrocytes. somatic cell problems

Inactive Publication Date: 2007-03-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Modern cell transplantation therapy has opened a new avenue for the treatment of demyelinating diseases and spinal cord injuries, but cell transplantation therapy has remained experimental due to the inability to obtain large, inexpensive, and purified oligodendrocyte precursor cells. stage and cannot be used clinically

Method used

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  • Application of cumarin kind compound in preparation of medicine for inducing nerve stem cell orienting differentiation
  • Application of cumarin kind compound in preparation of medicine for inducing nerve stem cell orienting differentiation
  • Application of cumarin kind compound in preparation of medicine for inducing nerve stem cell orienting differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Preparation of 7-hydroxycoumarin and double daphnetin

[0029] 11Kg of Daphne giraldii Nitsche. The root bark and stem bark of Daphne giraldii Nitsche. were crushed and extracted three times with 95% ethanol under reflux, the extracts were combined, the ethanol was recovered under reduced pressure to a volume of 10L, filtered, and the filtrate was washed with petroleum ether and chloroform in turn , ethyl acetate and n-butanol extraction, each extraction part is collected and concentrated into an extract, wherein 200 g of the extract part extracted by ethyl acetate is dissolved in methanol and filtered and then subjected to silica gel column chromatography, and gradient elution is carried out with chloroform-methanol. The eluted fractions were checked by thin-layer chromatography, and the fractions with the same single spot were combined and concentrated to obtain fractions 1, 2, 3, 4, 5, 6, 7, 8, and 9, of which fraction 2 was further subjected to a silica...

Embodiment 2

[0034] Embodiment 2: preparation scopoletin

[0035] 5Kg root bark of Daphne odora Thunb.var.atrocaulis Rehd., crushed and extracted by percolation with 75% ethanol to obtain 50L of extract, which was concentrated and then extracted with petroleum ether, chloroform, ethyl acetate and n-butanol , the extract is concentrated into an extract. The chemical components of each extraction part were systematically separated and identified by silica gel column chromatography, polyamide chromatography, Sephadex LH-20 and preparative HPLC. Twelve compounds were obtained by separation of chloroform, and one of them was scopoletin as light yellow powder after structural identification.

[0036] The structural formula of scopoletin is as follows:

[0037]

Embodiment 3

[0038] Embodiment 3: preparation of acarina

[0039] 5Kg of the rhizome of the medicinal material Azalea japonica is crushed and soaked in 80% ethanol for 24 hours, the soaking liquid is collected, concentrated until it has no alcohol smell, diluted with an appropriate amount of water to form a liquid extract, and extracted with petroleum ether, chloroform, ethyl acetate and n-butanol in sequence, Collect each extraction site and concentrate into an extract. Systematic chemical composition analysis was carried out on the extracts of various parts. As a result, 12 compounds were separated from the chloroform part. After structural identification, one of the light yellow powders was acarina.

[0040] The structure of agarose is as follows:

[0041]

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PUM

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Abstract

The invention relates to a method for using coumarin compound to prepare induced nerve stem cell directional differentiation drug. Wherein, said compound can be aesculetin, 7-hydroxycoumarin, megeresin, aesculetin, and scopoletin, etc. The test has proved that it has significant induced nerve stem cell directional differentiation function. And the prepared medicine has coumarin compound as active component, with acceptable carrier, while the active component content is 0.1-99.5%, and the inventive drug can be tablet, injection, etc.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of a coumarin compound in the preparation of drugs for inducing neural stem cell directional differentiation. Background technique [0002] Neural stem cells, as one of the main stem cells, refer to cells that have the ability to differentiate into neurons, astrocytes and oligodendrocytes, can self-renew, and are sufficient to provide a large number of brain tissue cells. Research findings in recent years have proved that neural stem cells widely exist in the central nervous system of adult mammals. In the brain of adult mammals, the two regions that can produce neurons and proliferate actively are the anterior subventricular zone and the dentate gyrus of the hippocampus. Subgranular. [0003] Since neural stem cells have lifelong self-renewal ability, they can keep constant in number, do not need a feeder cell layer in in vitro culture, and can be a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P25/00A61P43/00
CPCA61K31/37A61P25/00A61P43/00
Inventor 何成张卫东徐晓辉张薇苏娟张川
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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