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Magnetic sorting material and producing method and application thereof

A technology of magnetic separation and polymer materials, applied in the field of magnetic materials for the separation of biological samples, can solve the problem of low magnetic induction intensity, and achieve the effect of uniform size, regular shape and low coercive force

Inactive Publication Date: 2007-01-03
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low magnetic induction intensity generated by the ordinary permanent magnet magnetic field, it is difficult to achieve fast and efficient separation of target isolates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of magnetic separation pellets: prepared by splash quenching method.

[0039] Take three parts of soft iron material, its composition and weight percentage are respectively: iron 99.0% + carbon 0.04%, iron 99.5% + carbon 0.001%, and iron 99.8% + carbon 0.01%, and the balance contains a very small amount of aluminum, Sodium, zinc, chromium, nickel, copper, manganese and other elements. The three materials are melted at a high temperature of 1500°C-1800°C, and the molten soft iron is sprayed at a speed of 3-50 m / s, dropped from a high altitude or centrifugally thrown out, and the surface tension of the liquid phase is used to automatically shrink into a ball. Falling into the water, it suddenly condenses into a small iron ball.

Embodiment 2

[0041] Particle size screening: Most of the particle sizes of the three kinds of material pellets prepared in Example 1 are 0.2-1mm, and the magnetic properties of the iron balls are different with different particle sizes. The present invention adopts a sampling sieve to carry out particle size screening: a sampling sieve with a suitable mesh number is selected to screen pellets with target particle sizes of 0.01-0.1mm, 0.1-0.2mm and 0.2-1mm respectively. Iron balls with a larger particle size of 0.2-1 mm can be used to process a large number of samples, and are suitable for separating magnetically labeled biological samples with strong magnetic responses. Iron balls with a smaller particle size of 0.01-0.1 mm or 0.1-0.2 mm can generate a magnetic field with a larger gradient, which is suitable for separating magnetically labeled biological samples with weak magnetic response.

[0042]Density Screening: The main component of pellets is iron. During the preparation of pellets...

Embodiment 3

[0045] Improving magnetic permeability: The stress generated by quenching makes the magnetic properties of the balls unable to meet the requirements of sorting materials. It is necessary to eliminate the stress through a suitable annealing process, so as to improve the magnetic permeability of the small iron ball and reduce its coercive force. Raise the temperature to 400-900°C with the furnace, control the temperature to 700-1000°C, keep it for 1-5 hours, and cool it down to 400-900°C at a controlled speed, preferably raise the temperature to 800°C with the furnace, control the temperature to 900°C, and keep it for 4 hours. Cool down to 500°C at controlled speed. In order to avoid the oxidation of the balls, the annealing of the balls is carried out in a vacuum furnace or a hydrogen atmosphere. The annealed pellets have fine grains, uniform structure, low hardness, low coercive force (Hc=30~150A / m) and high maximum magnetic permeability (μ max =4000~9000), which meets the b...

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Abstract

The invention relates to a kind of magnetic sorting material and its preparation method and supplication. The material includes by weight 99.0-99.8% Fe, 0-0.04% C and some impurity. The product coercive force is 30-150A / m and the maximum magnetic conductivity is between 4000 and 9000. The invention supplies a method of processing the magnetic material into uniform pellets. The pellet possesses the magnetic character of high magnetic conductivity, low coercive force and low magnetism left and so on. The invention relates to the supplication of processing the magnetic sorting material into the magnetic sorting pellet. The pellet and the magnetic particle marked by the biologic molecular compose the magnetic sorting system. It can sort all kinds of biologic samples, such as cell, subcellular substance, bacterium, virus, nucleic acid, protein and so on.

Description

technical field [0001] The invention belongs to the field of magnetic materials, and more specifically relates to magnetic materials applied to the separation of biological samples. Background technique [0002] With the rapid development of modern biotechnology, magnetic separation technology has been widely used in the separation of biological samples. The principle is: the surface modification of magnetic beads (particle size <5 μm) can recognize and bind cells and subcellular substances Biomolecules of biological samples such as bacteria, viruses, nucleic acids or proteins form magnetic bead-biomolecular complexes. The complex is used to capture the target biological sample, and then retained under the action of an external magnetic field to achieve separation. Due to the low magnetic induction intensity generated by the ordinary permanent magnet magnetic field, it is difficult to achieve fast and efficient separation of target isolates. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): H01F1/09H01F41/02C12N13/00C12Q1/24
Inventor 沈鹤柏周海清兰天张诗渊
Owner SHANGHAI NORMAL UNIVERSITY
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