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Assaying method of Chinese medicine laying particle active principle and content

A technology of active ingredients and determination methods, applied in the field of analytical chemistry, can solve the problems of identification and content determination of active ingredient peptides in traditional Chinese medicine Luying granules, poor accuracy, low sensitivity, etc., and achieve accurate identification and content of active ingredient peptides The effect of determination, elimination of interference, and simple method

Inactive Publication Date: 2006-09-20
FUDAN UNIV
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  • Application Information

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Problems solved by technology

[0003] The known technology generally uses Coomassie Brilliant Blue method to determine the content of active ingredient polypeptide in traditional Chinese medicine Luying Granules. This method has many shortcomings, such as: poor accuracy, low sensitivity, and many interferences.
So far, no reliable method has been reported for the identification and content determination of the active ingredient polypeptide in the traditional Chinese medicine Luying Granules

Method used

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  • Assaying method of Chinese medicine laying particle active principle and content
  • Assaying method of Chinese medicine laying particle active principle and content
  • Assaying method of Chinese medicine laying particle active principle and content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Identification of the active ingredient polypeptide of traditional Chinese medicine Luying Granules

[0017] Chromatographic conditions:

[0018] Agilent 1100 HPLC / MS (including G1322A Vacuum Degasser, G1312A Bin Pump, G1313A ALS Autosampler, G1316AThermostatted Column Compartment, G1315B Diode Array and Multiple Wavelength Detector, DAD diode Array detector, G1946D (SL) Qudarpole LC / MSD quadrupole mass spectrometer, G2710AA single instrument ChemStation for liquid chromatography (LC) systems (chromatographic workstation), Agilent Zorbak, Eclipse C18 (150 × 2.1mm I.D., 5 μm); mobile phase: A: 0.1% trifluoroacetic acid water, B: 0.1% trifluoroacetic acid acetonitrile, 0 to 120 minutes A: B changed from 95:5 to 70:30; flow rate: 0.5ml min -1 ; Detection wavelength: 220nm; Column temperature: 25°C; Injection 5 μl.

[0019] Mass Spectrometry Conditions:

[0020] Ionization method: API-ES; fragmentation voltage: 70ev; detection method: positive ion full scan 100...

Embodiment 2

[0025] The mensuration of embodiment 2 traditional Chinese medicine Lu Ying granule active ingredient polypeptide content

[0026] Chromatographic conditions:

[0027] American Angilent HP-1100 high performance liquid chromatograph, including online degassing, quaternary pump, column thermostat, ultraviolet detector, HP chromatography workstation and 20μL manual sampling system; chromatographic column: Agilent Zorbak, Eclipse C 18 (150×4.6mm I.D., 5μm); Column temperature: 25°C; Mobile phase: A: 0.1% trifluoroacetic acid aqueous solution, B: 0.1% trifluoroacetic acid acetonitrile, A: B changed from 95:5 to 0 to 60 minutes 70:30; flow rate: 1.0mL / min; detection wavelength: 220nm. For a typical chromatogram see figure 1 .

[0028] Preparation of the reference substance solution: Accurately weigh an appropriate amount of the reference substance oxytocin (nonapeptide), add water to make a solution containing 0.25 mg per 1 ml, and obtain

[0029] The preparation of need testing...

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Abstract

A method for discriminating the active component of Chinese elder flower particle and measuring its content features that the HPLC / MS method is used to discriminating the polypeptide as its active component and the efficient liquid-phase chromatography is used to measure the content of polypeptide.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for determining the effective components and content of traditional Chinese medicine Luying granules. Background technique [0002] Chinese patent medicine Luying, its Luying granule dosage form, has been on the market for many years. The main active ingredient is Xiaofen polypeptide, which is brown-yellow or tan granules, sweet and slightly bitter. Widely used in clinical internal medicine, it has the effects of soothing the liver and strengthening the spleen, promoting blood circulation and removing blood stasis, reducing swelling and relieving pain. [0003] The known technology generally uses the Coomassie Brilliant Blue method to determine the content of the active ingredient polypeptide in the traditional Chinese medicine Luying Granules. This method has many shortcomings, such as: poor accuracy, low sensitivity, and many interferences. So far, ther...

Claims

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Application Information

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IPC IPC(8): A61K36/35A61K9/16A61P29/00G01N30/02
Inventor 段更利黄滔敏刘振
Owner FUDAN UNIV
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