Composite collagen-base rack material and its preparation and use

Abstract

The invention relates to a collagen-based composite scaffold material and its preparation method and application. The material includes collagen and water-soluble polysaccharide. The water-soluble polysaccharide is at least one of chitosan, seaweed polysaccharide salts, proteoglycan, cellulose, carrageenan, gum arabic and starch. The collagen has a three-dimensional network structure of natural fibers. The preparation method of the bracket material is as follows: choose mammalian skin as raw material, remove the subcutaneous tissue with a splitter, then soak in water, degrease, lime, delime, split, soften, compound, and sterilize by cobalt 60 irradiation. Adding functional materials to the collagen-based composite support material can be used to prepare biological dressings, artificial epidermis, artificial dermis, and artificial composite skin, and the collagen-based composite support material can also be used in conjunction with ointments. The bracket material has good permeability, water vapor permeability and absorption.

Description

technical field [0001] The invention relates to the fields of chemical engineering, medicine, material science and bioengineering, in particular to a collagen-based composite scaffold material and its preparation method and application. Background technique [0002] Scaffold materials for skin tissue engineering can be divided into three categories: synthetic scaffold materials, biological scaffold materials and composite scaffold materials. Synthetic materials have high tensile strength, degradation rate and microstructure are easy to control during the synthesis process, but their histocompatibility, cell affinity, water absorption, wound adhesion, etc., need to be improved by appropriate chemical and biological methods performance. For example, the polyurethane scaffold material has poor water absorption, which makes the wound surface prone to fluid accumulation and bacteria to multiply, and does not have the function of regulating cell growth and promoting wound healing...

AI Technical Summary

Problems solved by technology

Synthetic materials have high tensile strength, degradation rate and microstructure are easy to control during the synthesis process, but their histocompatibility, cell affinity, water absorption, wound adhesion, etc., need to be improved by appropriate chemical and biological methods performance

For example, polyurethane scaffold materials have poor water absorption, which makes the wound prone to fluid accumulation and bacteria to multiply, and does not have the function of regulating cell growth and promoting wound healing

Biomaterials have good biocompatibility and are easy for cell attachment and growth, but the degradation rate is fast and the microstructure is not easy to control, etc.

For example, although the collagen gel scaffold material can well promote wound recovery, its mechanical properties are poor, it is brittle when dry, it is easily broken after absorbing wound exudate, and it is easy to degrade under the action of collagenase, so it cannot play a good role. Protective effects

Collagen gel, a binary copolymer of glycolide and lactide, is used as an artificial skin scaffold material. Although the tensile strength is improved, the small molecules after polymer degradation form an acidic environment locally, which is not conducive to cell growth.

[0003] The US Patent No. US 5282,859 introduces a double-layer artificial skin. The epidermis is a high-purity microporous collagen gel film, and the dermis is a porous collagen sponge cultured with fibroblasts. Glutaraldehyde crosslinking improves its Tensile strength, but its tensile strength can not meet the requirements of clinical application, and the degradation rate is too fast, glutaraldehyde is toxic to cells

The Chinese patent No. CN 1387,923 discloses a heterogeneous acellular dermal scaffold and its preparation method. The scaffold is obtained by treating suckling pig skin with trypsin and glutaraldehyde. It has high tensile strength and a fast degradation rate. control, good tissue compatibility, but poor permeability, easy to produce fluid and gas accumulation on the wound surface, and slow vascularization

The Chinese patent with the patent number CN 1343,523 discloses a microporous allogeneic or heterogeneous acellular dermal substitute that can be used as a wound repair, using mechanical or laser methods to regularly stamp dense penetrating dermis on the acellular dermis. Pores, although this can solve the shortcomings of poor permeability of acellular dermal substitutes, but the non-fibrous components in the dermal tissue are not completely removed, and its surface bioactivity and absorption are poor