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Method and system for hyperaccumulating solid-phase denitrifying flora based on nanofiber carbon source

A nanofiber and nanofiber membrane technology, applied in the field of natural water body remediation, can solve the problems of low use efficiency, carbon source does not have the ability to release carbon, non-biodegradable, etc., to avoid short flow, achieve ultra-enrichment, good hydraulic power The effect of the condition

Active Publication Date: 2022-07-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the hollow fiber membrane bundle of this technology does not provide a carbon source, so it does not have the ability to release carbon, and it is not biodegradable, so it needs to be replenished with a liquid carbon source, which results in a certain cost of chemicals and an additional load on the water output index.
In essence, this technology uses the micropores on the fiber to trap the bacteria. The pores on the fiber reach the micron level and do not have a large specific surface area. It only relies on the outer surface of the fiber bundle to attach the bacteria, which is easy to foul the membrane and cause the use efficiency. Low, so it is impossible to achieve super-enrichment of bacterial flora

Method used

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  • Method and system for hyperaccumulating solid-phase denitrifying flora based on nanofiber carbon source
  • Method and system for hyperaccumulating solid-phase denitrifying flora based on nanofiber carbon source
  • Method and system for hyperaccumulating solid-phase denitrifying flora based on nanofiber carbon source

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Embodiment 1

[0038] The system provided by the present invention based on the nanofiber carbon source super-enriched solid anti-nitrifying bacteria group is as follows: figure 1 As shown, it includes a pre-inoculation device 1, and a water inlet pool 4, an inlet water pump 6, a water inlet buffer zone 7, a core enrichment zone 8, and a water outlet 10 connected in sequence;

[0039] Wherein, the pre-inoculation device 1 includes a high-efficiency inoculator 2 and a vacuum pump 3 at the bottom thereof; the water inlet pool 4 is connected to the water inlet buffer zone 7 through the inlet water pump 6; The support plates 9 are arranged at intervals in parallel grids.

[0040] The upper part of the high-efficiency inoculator 2 is an open cuboid, the lower part is an inverted quadrangular pyramid shape, the upper part and the lower part are connected with a perforated plate 11, and the bottom water outlet pipe of the lower part is connected to the Buchner funnel 12, and the Buchner funnel 12 i...

Embodiment 2

[0046] The method for super-enriched solid anti-nitrifying bacteria based on nanofiber carbon source provided by the present invention is:

[0047] S1. First, fix the activated sludge with the help of the pre-inoculation device 1: select PHB and PBAT for physical blending with a mass ratio of 1:1, and mix DMF and chloroform with a volume ratio of 1:1 as a solvent. Mix well at room temperature to make a 10% (wt / v) polymer solution. The working distance of electrospinning was 20 cm, the working voltage was 30 KV, the ambient temperature was 25°C, and the ambient humidity was 40% RH, and the solid-phase carbon source was made into a nanofiber membrane. The selected nanofibers have a diameter of 500 nm and a porosity of 80%.

[0048] The solid-phase carbon source nanofiber membrane is spread on the orifice plate of the high-efficiency inoculator 2, and the fresh secondary sedimentation tank sludge and the culture solution are mixed according to the volume ratio of 1:1 as the inoc...

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Abstract

The method comprises the following steps: S1, physically blending PHB / PBAT, preparing a solid-phase carbon source nanofiber membrane by using an electrostatic spinning method, flatly laying the solid-phase carbon source nanofiber membrane on a pore plate of an efficient inoculator, uniformly coating the surface with activated sludge of a secondary sedimentation tank, and drying the solid-phase carbon source nanofiber membrane to obtain a solid-phase carbon source nanofiber membrane; performing preliminary fixation and inoculation on the activated sludge by means of vacuum filtration; s2, covering a supporting plate with the inoculated nanofiber membrane in a rolling manner, and arranging the nanofiber membrane in a core enrichment area in a parallel grid type at intervals; s3, after the culture solution is uniformly distributed in the water inlet buffer area, the culture solution enters the core enrichment area to be in full contact with the nanofiber solid-phase carbon source inoculated with the sludge for denitrification. The solid-phase carbon source nanofiber membrane disclosed by the invention is biodegradable and can continuously release a carbon source to provide electrons; the exponentially multiplied pores and specific surface area are beneficial to attachment of microorganisms; the water flow is parallel to the nanofiber membrane, and the hydraulic impact on the biological membrane is weakened, so that the hyperaccumulation of the solid-phase denitrification flora in the activated sludge is realized.

Description

technical field [0001] The invention relates to the technical field of natural water body remediation, in particular to a method and a system for super-enriching solid anti-nitrifying bacteria based on a nanofiber carbon source. Background technique [0002] The non-point source pollution of nitrogen elements aggravates the eutrophication of water bodies, deteriorates the water quality of lakes and endangers human health. For this reason, my country has formulated strict discharge standards. Conventional physical and chemical treatment methods and denitrification processes in sewage treatment plants are essentially point source treatment. Their high cost, complex equipment, and secondary pollution from chemical dosing limit their treatment of surface source pollution. In addition, the nitrogen concentration in non-point source polluted water is low, the water volume fluctuates greatly, and the distribution range is wide. These practical factors also determine that the tradit...

Claims

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Application Information

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IPC IPC(8): C02F3/28C12N11/096C12M1/26C12M1/12C12M1/00C02F101/16
CPCC02F3/2806C12N11/096C12M25/02C12M33/14C12M33/00C02F2101/16Y02W10/10
Inventor 李世阳孙悦唐量吴明红
Owner SHANGHAI UNIV
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