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Preparation method and application of pH and thermal response type CuAu nano assembly

A nano-assembly, thermally responsive technology, applied in the field of biomedical engineering material preparation, can solve problems such as damage to healthy tissue, and achieve the effects of not easily oxidized, increased copper ion release, and efficient photothermal sterilization

Active Publication Date: 2022-04-29
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in photothermal therapy, non-localized heat and uncontrollable temperature and ion release often cause great damage to healthy tissue

Method used

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  • Preparation method and application of pH and thermal response type CuAu nano assembly
  • Preparation method and application of pH and thermal response type CuAu nano assembly
  • Preparation method and application of pH and thermal response type CuAu nano assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of copper-gold nanoparticles coated with oleylamine

[0047] (1) Preparation of gold nanoparticles: Dissolve 200mg tetrachloroauric acid trihydrate in a mixed solution of 10mL oleylamine and 10mL 1,2,3,4-tetralin, feed an inert gas, and ℃ for 30-60 minutes to remove water vapor and oxygen in the reaction system, add 90mg of tetrabutylammonium bromide dissolved in 1mL oleylamine and 1mL 1mL 1,2,3,4-tetralin mixed solution, the solution is prepared by The golden yellow color turns purple. After the reaction is continued for 1 hour at 2-5°C, the nanoparticles are precipitated with acetone, centrifuged at 8000 rpm for 5 minutes, the supernatant is discarded, the precipitate is dissolved in n-hexane, and then centrifuged with ethanol to obtain gold nanoparticles.

[0048] (2) Preparation of secondary-growth gold nanoparticles: Dissolve 100 mg tetrachloroauric acid trihydrate in a mixed solution of 6 mL oleylamine and 6 mL octadecene, feed inert gas, an...

Embodiment 2

[0050] Example 2: Preparation of copper-gold nanoparticles coated with oleylamine

[0051] The synthesis was carried out with reference to the preparation process of Example 1, except that the copper acetylacetonate added in the step (3) was changed to 0-200 mg, and copper-gold nanoparticles coated with oleylamine were also obtained. Wherein, the copper-gold ratios of the copper-gold nanoparticles coated with oleylamine are respectively Cu:Au=0˜5:1.

Embodiment 3

[0052] Example 3: Synthesis of pH- and heat-responsive small molecules containing thiols

[0053] N-tert-butoxycarbonyl-glycine (525 mg, 3 mmol), 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (1719 mg, 9 mmol) and N-hydroxysuccinimide Amine (1035mg, 9mmol) was sequentially added to anhydrous N,N-dimethylformamide solution (10mL) and activated for half an hour. Cystamine (152.0 mg, 1 mmol) was then added to anhydrous N,N-dimethylformamide solution, and the resulting mixture was stirred under nitrogen at room temperature for 24 h. The reaction was carried out overnight at room temperature under nitrogen protection, and the progress of the reaction was detected by thin-layer chromatography. The reaction was considered complete when the raw material spots on the chromatographic plate disappeared. The solvent was removed by rotary evaporation, the product was dissolved in dichloromethane, washed with water three times, dried and purified by column chromatography (elue...

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PUM

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Abstract

The invention discloses a preparation method and application of a pH and thermal response type CuAu nano assembly. The preparation method comprises the following steps: preparing oleylamine coated copper-gold nanoparticles; preparing a sulfydryl-containing pH and thermal response type small molecule; carrying out ligand exchange on the oleylamine coated copper-gold nanoparticles to obtain monodispersed copper-gold nanoparticles; and preparing the pH and thermal response type copper-gold nano assembly. The pH and thermal response type copper-gold assembly has good photo-thermal performance, and on the basis that a photo-thermal anti-biofilm is achieved, damage to normal tissue around a wound due to excessive heating is reduced; meanwhile, deep penetration and release of copper ions are promoted, and the effects of resisting bacteria and promoting wound healing are achieved.

Description

technical field [0001] The invention relates to a preparation method and application of a biomedical engineering material, in particular to a preparation method and application of a pH- and heat-responsive CuAu nano assembly. Background technique [0002] In recent years, the incidence of chronic wound healing complicated by diabetes and other diseases has been increasing, becoming a major medical burden worldwide. When superinfection occurs in chronic wounds, treatment often fails due to the presence of biofilm at the wound site and release of endotoxins that slow the healing process. Biofilm is a bacterial community with an extracellular polymer (EPS) matrix formed by the adhesion and proliferation of microorganisms. The exopolysaccharide in the extracellular polymer acts as a physical barrier against innate immune cell attacks and antibiotic penetration, thus greatly increasing the traditional Difficulty of antibiotic treatment. [0003] Different from traditional chemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K41/00A61K47/18A61P17/02A61P31/04B82Y5/00
CPCA61K9/5123A61K41/0052A61P31/04A61P17/02B82Y5/00Y02A50/30
Inventor 孙晓莲赵桂桢吴晓静
Owner CHINA PHARM UNIV
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