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Application of GMFB as insulin resistance biomarker

A technology for insulin resistance and biomarkers, which can be used in biological testing, biological material analysis, medical preparations containing active ingredients, etc., and can solve problems such as no GMFB yet.

Pending Publication Date: 2021-12-07
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of clinically effective treatments for insulin resistance. Commonly used insulin sensitizers thiazolidinediones (TZDs) can increase the metabolism of fatty acids in the body, promote glucose utilization, and reduce fasting blood sugar, but they are often accompanied by obesity, Headache, edema and other adverse reactions
There is no research on the relationship between GMFB and adipocyte insulin resistance

Method used

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  • Application of GMFB as insulin resistance biomarker
  • Application of GMFB as insulin resistance biomarker
  • Application of GMFB as insulin resistance biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] GMFB expression detection.

[0057] After the SD rats were killed by neck dislocation, a T-shaped incision was made on the chest and abdomen, and the white adipose tissue in the groin was taken by hand, and then the tissue protein was extracted. The expression level of GMFB was detected.

[0058] figure 1 It shows that GMFB expression in adipose tissue increases after high-fat diet.

Embodiment 2

[0060] Insulin concentration detection.

[0061] The plasma of SD rats in each group was collected and tested by ELISA:

[0062] After the SD rat was anesthetized, it was killed by dislocation of the neck, the chest cavity was opened, and blood was taken from the heart, which was taken into an EP tube containing EDTA-anticoagulant, then centrifuged at 2000-3000rpm for 15-20 minutes, and the supernatant was drawn to obtain a light yellow color plasma;

[0063] The obtained plasma was subjected to an ELISA experiment to detect the plasma insulin concentration, and the changes of the plasma insulin concentration were compared. The operation is as follows: take out the required enzyme label strip, add 100ul of sample, and set up zero hole (sample dilution), standard hole, and sample hole to be tested respectively. Cover the ELISA plate, place in a wet box, incubate at 37°C for 120 minutes; discard the liquid, wash 4 times; add 100ul of 1×HRP-labeled detection antibody to each we...

Embodiment 3

[0066] Glucose tolerance test.

[0067] Glucose tolerance test was carried out after 12 weeks of high-fat diet and normal diet in SD rats. The specific operation is as follows: SD rats are intraperitoneally injected with 20% glucose (1 g / kg), and then the blood glucose concentration is measured with a Yuyue blood glucose meter at 0, 15, 30, 60, 90, and 120 min. The result is as image 3 as shown, image 3 It is a schematic diagram of glucose tolerance detection in wild-type SD rats and GMFB gene-knockout SD rats under the condition of normal diet and high-fat diet for 12 weeks.

[0068] image 3 The results showed that GMFB gene knockout improved the blood sugar rise caused by insulin resistance in rats fed a high-fat diet, and the area under the curve of the glucose tolerance test was significantly reduced, which improved glucose tolerance.

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Abstract

The invention relates to the technical field of treatment of insulin resistance, and in particular relates to application of a cytokine GMFB, and application of the GMFB as a biomarker of insulin resistance. Experiments discover that GMFB as an inflammatory factor is high in expression in an insulin resistance state caused by high fat diet and is not expressed or extremely low in expression in a normal condition, which indicates that GMFB plays a role in adipose tissues in the insulin resistance state and can inhibit the functions of the adipose tissues. The plasma insulin concentration of a high-fat diet rat after GMFB gene knockout is obviously reduced compared with that of a wild type high-fat diet rat, which indicates that the GMFB gene knockout can improve insulin resistance caused by high-fat diet; GMFB gene knockout obviously improves the glucose tolerance of SD rats; the insulin tolerance is improved; the functions of fat cells are improved.

Description

technical field [0001] The invention relates to the field of insulin resistance treatment, in particular to the use of a cytokine GMFB, and the application of GMFB as a biomarker of insulin resistance. Background technique [0002] With the development of society, the improvement of living standards, and the change of lifestyle, dietary structure has changed significantly, and the number of obese people has also increased sharply. With the rising rate of obesity, type 2 diabetes is on the rise globally, and has become the third chronic non-communicable disease that threatens human health after cancer and cardiovascular disease. Type II diabetes has a high prevalence rate and long course of disease, and is often accompanied by serious complications, such as diabetic nephropathy, diabetic cardiovascular disease and retinopathy, which not only seriously affect the quality of life of patients, but also bring great harm to patients' families and society. Therefore, the preventio...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/74G01N33/58A61K38/17A61K45/00A61P3/10
CPCG01N33/6893G01N33/74G01N33/581A61K45/00A61K38/1709A61P5/50A61P3/10G01N2333/62G01N2800/042G01N2800/50
Inventor 吕立夏徐国彤徐金媛施思朱彤王娟高芙蓉张介平金彩霞田海滨徐晶莹欧庆健
Owner TONGJI UNIV
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