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Polypeptide nanogold composite material and antibody penicillin receptor modified glassy carbon electrode and application thereof

A glassy carbon electrode and composite material technology, applied in the direction of material electrochemical variables, material analysis, material analysis through electromagnetic means, etc., can solve the problems of expensive instruments, complicated pretreatment, low repeatability, etc., and achieve good application potential , good reproducibility, good repeatable effect

Pending Publication Date: 2021-11-12
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography, for example, although accurate and sensitive, requires skilled personnel and complicated sample pretreatment, while the instrument is relatively expensive
Immunoassay methods, such as enzyme-linked immunoassay, have the advantage of being easy to operate and suitable for batch processing, but their repeatability is low and they are easily interfered by various factors.

Method used

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  • Polypeptide nanogold composite material and antibody penicillin receptor modified glassy carbon electrode and application thereof
  • Polypeptide nanogold composite material and antibody penicillin receptor modified glassy carbon electrode and application thereof
  • Polypeptide nanogold composite material and antibody penicillin receptor modified glassy carbon electrode and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of Polypeptide Nanogold Composite Material and Glassy Carbon Electrode Modified by Antibody Penicillin Receptor

[0062] It includes the following steps:

[0063] Step 1, the bare glassy carbon electrode is ground and polished on alumina powder, then ultrasonicated in absolute ethanol and water in sequence, rinsed with water and dried in the air. When measuring the polishing effect, use cyclic voltammetry (CV) to measure the potential difference between the oxidation peak and the reduction peak. If the potential difference between the two peaks is less than 90mV, it means that the electrode polishing effect is good;

[0064] Step 2, use a pipette gun to draw 20 μ LC16R4-AuNPs and evenly drop-coat it on the surface of the bare glassy carbon electrode treated in step 1, and then place it in a 37°C incubator for 20 minutes until the modification solution is completely dry, and then wash the surface with PBST buffer solution , shake off the water drop...

Embodiment 2

[0068] Embodiment 2 Modifies the evaluation of electrode performance:

[0069] In order to explore the behavior of electrochemical sensors, cyclic voltammetry (CV) was used to detect and analyze the electrodes of different modification steps, and explore the results of bare GCE, C16R4-AuNPs / naked GCE, antibody / C16R4-AuNPs / naked GCE, BSA / antibody / The electrochemical response signals of the electrochemical sensors with five modified electrodes, C16R4-AuNPs / naked GCE, receptor / BSA / antibody / C16R4-AuNPs / naked GCE, were different. Such as figure 1 As shown, the reduction peak current intensities on different modified electrodes are different. There is an obvious reduction peak on the bare electrode; on C16R4-AuNPs / bare GCE and antibody / C16R4-AuNPs / bare GCE, the current value is partially enhanced, and the peak potential has a slight positive shift. The reduction peaks on BSA / antibody / C16R4-AuNPs / naked GCE and acceptor / BSA / antibody / C16R4-AuNPs / naked GCE are significantly enhanced,...

Embodiment 3

[0071] Example 3 Modified electrode for detection of AMP:

[0072] Differential pulse voltammetry (DPV) was used to investigate the response signal of the electrochemical sensor to AMP by modifying the electrode of antibody and receptor and only the receptor, and to explore which of the two modified electrodes was better. Such as image 3 As shown, the response signal of the electrode modified with the antibody and the receptor changed significantly compared with the electrode modified only with the receptor, and the peak potential shifted slightly positively. To sum up, compared with the electrodes of the modified antibody and receptors constructed by the present invention, the response signal of β-lactam antibiotics can be significantly improved, specifically, the peak current is significantly enhanced, Second, the peak potential shifted positively. Therefore, the modified electrode has a promising application prospect in the detection of β-lactam antibiotics in practice. ...

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Abstract

The invention discloses a glassy carbon electrode modified by a polypeptide nanogold composite material and an antibody penicillin receptor, and a method for detecting [beta]-lactam antibiotics by using the electrode, and belongs to the field of food detection. The preparation method comprises the following steps: uniformly dispensing a C16R4-AuNPs nano composite material on the surface of a pretreated GCE electrode to generate C16R4-AuNPs / GCE, then dropwise adding a penicillin antibody on the surface of the electrode, incubating, then cleaning the surface, and washing away the uncombined antibody; dropwise adding BSA (bovine serum albumin) on the surface of the electrode, and incubating; and dropwise adding a penicillin receptor on the surface of the electrode, and incubating to obtain the glassy carbon electrode modified by the polypeptide nanogold composite material and the antibody penicillin receptor. The electrode has a relatively good detection limit and a relatively wide linear range on the [beta]-lactam antibiotics, the detection sensitivity of the [beta]-lactam antibiotics is obviously improved, and meanwhile, the electrode has good anti-interference capability and selectivity.

Description

technical field [0001] The invention relates to the detection of β-lactam antibiotic residues in milk, in particular to a polypeptide nano-gold composite material and a glassy carbon electrode modified by an antibody penicillin receptor, and a method for detecting β-lactam antibiotics by using the electrode, which belongs to food detection field. Background technique [0002] β-lactam antibiotics are mostly used to treat bacterial infections, especially in veterinary medicine, β-lactam antibiotics are usually used to treat mastitis in cows. The abuse of antibiotics has brought potential dangers to dairy products such as milk, and the passive intake of these drugs will bring many adverse symptoms such as diarrhea, vomiting, and allergies to consumers. Dairy products are now an important nutritious food for people, and the continuous increase in per capita consumption has also increased the risk of health hazards to allergic people. The most serious thing is that the normal i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 毛烨炫张西亚党梦黄现青谢新华赵秋艳乔明武丁明月宋轲李子哲
Owner HENAN AGRICULTURAL UNIVERSITY
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