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Bio-energy active material and application thereof

An active material and biological technology, applied in the field of biomedical tissue engineering, can solve problems such as the inability to continuously improve cell stability and related biomass activity, hinder the application and development of bioenergy active materials, achieve unique bioenergy activity, and promote adhesion , the effect of long degradation time

Inactive Publication Date: 2021-10-26
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, 3D scaffolds with long-term bioenergy release effects for the repair of complex bone tissue defects have rarely been reported
This is largely due to the inability to continuously improve the stability of ATP in cells and related biomass activity using existing bioscaffold materials, hindering the application and development of bioenergetic active materials in bone tissue engineering.

Method used

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  • Bio-energy active material and application thereof
  • Bio-energy active material and application thereof
  • Bio-energy active material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) The microorganism Halomonas that can synthesize 3-hydroxybutyric acid-4-hydroxybutyric acid copolyester was fermented and cultured for 72 hours in 60MMG medium at 37°C and 400-800rpm, and collected after 72 hours The cells are placed at 70° C. to ventilate and dry the cells to obtain dry cell powder containing 3-hydroxybutyric acid-4-hydroxybutyric acid copolyester.

[0038] The composition of 60MMG medium is: glucose 30g / L, yeast extract 1g / L, ammonium sulfate 0.25g / L, magnesium sulfate 0.2g / L, disodium hydrogen phosphate 9.65g / L, potassium dihydrogen phosphate 1.5g / L, Trace element I 10ml / L, trace element II 1ml / L.

[0039] (2) extract the 3-hydroxybutyric acid-4-hydroxybutyric acid copolyester in the dried thallus powder with chloroform (1g dry thallus powder adds 20ml chloroform), pack in the autoclave after stirring evenly, in React at 100°C for 4 hours.

[0040] (3) After the autoclave is cooled, the cell fragments are removed by filtration or suction filtra...

Embodiment 2

[0047] The 3-hydroxybutyric acid-4-hydroxybutyric acid copolyester material synthesized in Example 1 was loaded into a melt 3D printer (180° C.) to prepare a porous scaffold for bone tissue repair.

[0048] The result is as figure 2 As shown, among them, figure 2 -A porous scaffold prepared for 3D printing, figure 2 -B is a scanning electron microscope image of the cross-section of the scaffold, and the pore diameter of the porous scaffold is about 350-400 μm.

experiment example

[0050] The 3D porous scaffold in Example 2 was soaked in phosphate buffer for 8 weeks, and the degradation products were collected. In phosphate buffer, the degradation product of 3-hydroxybutyric acid-4-hydroxybutyric acid copolyester is mainly 3-hydroxybutyric acid (3HB). The concentration of the collected degradation products was determined, and then the in vitro experiment was carried out.

[0051] The composition of the phosphate buffer is: sodium chloride 7.9g / L, potassium chloride 0.2g / L, potassium dihydrogen phosphate 0.24g / L.

[0052] (1) Human bone marrow mesenchymal stem cells (hBMSCs) in good growth state were taken, according to 2×10 4 The cell density was seeded in a 48-well plate. After 4 hours, different concentrations of 3-hydroxybutyric acid (0 μM, 10 μM, 40 μM, 80 μM, 160 μM, 320 μM) were added to the above cells. Cell proliferation was measured respectively. At the same time, lactic acid (LA) was used as the control group.

[0053] The result is as im...

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Abstract

The invention discloses a bio-energy active material and application thereof, and particularly discloses the bio-energy active material which is a biodegradable polymer. A degradation product of the bio-energy active material is a metabolic intermediate product in a tricarboxylic acid cycle and / or a glycolytic pathway, or a polymer monomer capable of being converted into the metabolic intermediate product in the tricarboxylic acid cycle and / or the glycolytic pathway, or a polymer monomer capable of being converted into acetyl coenzyme A. The degradation product of the bio-energy active material provides biological energy for tissue cells through the tricarboxylic acid metabolic cycle or the glycolysis pathway, so that the problem that a traditional biodegradable material cannot continuously improve the stability of ATP in cells and the activity of related biomass is solved; and the bio-energy active material has a wide application prospect in the field of bone tissue regeneration, especially in the aspect of large-segment bone defect repair.

Description

technical field [0001] The invention belongs to biomedical tissue engineering, and specifically relates to a bioenergy active material and its application. Background technique [0002] Clinically, various types of bone defects caused by trauma, infection, bone tumor resection and other reasons are very common. The number of bone transplant patients worldwide reaches 2.2 million every year [J.Van der Stok, E.M.Van Lieshout, Y.El-Massoudi, G.H.Van Kralingen, P.Patka, Bone substitutes in the Netherlands-asystematic literature review, Acta Biomater 7(2)(2011) 739-50.]. According to the statistics of the American Association of Orthopedic Surgeons (AAOS), 6.3 million people suffer from fractures in the United States every year, and among them, as many as 500,000 patients need to receive bone grafts. Lobb, B.R. DeGeorge, Jr., A.B. Chhabra, Bone Graft Substitutes: Current Concepts and Future Expectations, J Hand Surg Am 44(6)(2019) 497-505e2.]. In my country, there are more tha...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/56A61L27/50
CPCA61L27/18A61L27/56A61L27/50A61L2430/02C08L67/04
Inventor 张鹏李健
Owner SHENZHEN INST OF ADVANCED TECH
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