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Bacillus coagulans preparation and preparation method thereof

A technology of bacillus coagulans and preparations, applied in the field of bacillus coagulans preparations and its preparation, can solve the problems of high production cost not suitable for industrial application, lower actual utilization rate of probiotic preparations, complicated coating process, etc., and achieve recovery of microorganisms Effects of barrier function, inhibition of intestinal peristalsis, and shortening of disease course

Active Publication Date: 2021-10-22
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the preparation method of bacillus coating and spray drying, the coating material usually adopts raw materials such as β-cyclodextrin, carboxymethyl cellulose, gum arabic, etc. High production cost is not suitable for industrial application
Some Bacillus spores are coated and sprayed by ion exchange secondary coating method, which improves the stability of the Bacillus spore powder product to a certain extent, but the disadvantage is that the coating process is complicated, and it will affect the spore germination during use and delay The function of probiotics is hindered, and the actual utilization rate of probiotic preparations is reduced

Method used

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  • Bacillus coagulans preparation and preparation method thereof
  • Bacillus coagulans preparation and preparation method thereof
  • Bacillus coagulans preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Isolation and screening of embodiment 1 bacterial strain

[0039] 1. Primary screening of Bacillus

[0040] Take 1g of fermented sour cabbage, smash it with a masher, soak it with 10ml of 0.85% physiological salt for 10 minutes, put it into a sterile sample bag, beat it with a homogenizer, and pour it into a test tube, set the water bath at 70°C Heated for 10 minutes, and the suspension was diluted gradually, and 10 -1 、10 -2 、10 -3 Three dilution gradients of 100ul were spread on the MRS medium, and cultured in a 37°C incubator under aerobic conditions for 48h. After a single colony grew on the plate, microscopic examination was performed respectively. According to the results of microscopic examination, the applicant screened out 10 bacillus strains, which were named NH-1, NH-2, ..., NH-10 respectively.

[0041] 2. Re-screening of Bacillus

[0042] Liquid fermentation medium: weigh 3.0g yeast powder, 5.0 peptone, 2.0g beef extract, K 2 HPO 4 3g, MnSO 4 0.005g, ...

Embodiment 2

[0051] Example 2 strain identification

[0052] 2.1 Identification of colony morphology

[0053] The single colony of the NH-8 strain is milky white and shiny, the surface of the colony is smooth and moist, the edges are neat, and the diameter of the colony is 2-3mm. Under the microscope, it is rod-shaped, arranged in single, pair or chain, and the spores are terminal.

[0054] 2.2 Identification of physiological and biochemical characteristics

[0055] The preparation of the inoculum in this embodiment is as follows: under sterile conditions, take an appropriate amount of fresh bacterial solution, centrifuge at 5000rpm for 5min, wash 2 times with PBS buffer, then resuspend with the same volume of PBS buffer and dilute 50 times as the inoculum. liquid.

[0056] 1. Temperature growth range experiment

[0057] Under sterile conditions, the inoculum was inoculated into 10 mL of MRS liquid medium at a 10% inoculation amount, and 10 mL of MRS liquid medium without inoculation w...

Embodiment 3

[0114] Example 3 Bacillus coagulans VHProbi C08 Salinity Tolerance Test

[0115] Under sterile conditions, inoculate 10% of the inoculum into 5 mL of MRS liquid medium with salt concentrations of 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8%. In this method, 5mL of MRS liquid medium without inoculation was used as a control, and placed in a constant temperature shaking culture at 37°C to observe whether the medium became turbid.

[0116] The results showed that Bacillus coagulans VHProbi C08 grew at a salt concentration of 1% to 7%, and did not grow at a salt concentration of 8%. The maximum salt concentration of Bacillus coagulans VHProbi C08 was 7%.

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Abstract

The invention relates to the technical field of functional microorganisms, and particularly provides a bacillus coagulans preparation and a preparation method thereof. The bacillus coagulans preparation is prepared by carrying out spray drying on a mixed solution of bacillus coagulans and a coating material, is high in viable count, good in tolerance to artificial gastric acid and intestinal juice and very high in heat resistance, and can be widely applied to the fields of foods, health-care products and the like.

Description

technical field [0001] The invention relates to the technical field of functional microorganisms, in particular to a bacillus coagulans preparation and a preparation method thereof. Background technique [0002] According to the definition of ISAPP (International Scientific Association Probiotic and Prebiotic), probiotics refer to living microorganisms that can have a beneficial effect on the health of the host when administered in sufficient quantities. At present, the probiotics for humans mainly include Lactobacillus, Bifidobacterium, Enterococcus, E. coli, Bacillus, Escherichia coli, Streptococcus thermophilus and yeast. The most widely used genera are Bifidobacterium and Lactobacillus. At the same time, FHO / WHO stipulates that the minimum number of viable bacteria for probiotics to play a role is not less than 10 6 CFU / mL, but the most critical problem existing in probiotic preparations is that the number of viable bacteria decreases rapidly during processing, transpo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135C12R1/07
CPCC12N1/20A23L33/135A23V2002/00A23V2200/3204A23V2250/5488A23V2250/5118A23V2250/628A23V2250/5028A23V2250/284Y02A50/30
Inventor 段治崔洪昌张景燕郭超群张陆霞
Owner QINGDAO VLAND BIOTECH INC
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