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Application of human amniotic epithelial stem cells in preparation of medicine for treating acute kidney injury

A technology for acute kidney injury and human amniotic membrane, applied in the biological field, can solve the problems of short residence time, formation of teratoma, abnormal differentiation, etc., and achieve the effect of reducing the source, easy to obtain materials, and strong proliferation ability.

Pending Publication Date: 2021-10-22
PEKING UNIV FIRST HOSPITAL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing AKI treatment research, the following stem cells from different sources are used, and the problems are as follows: ① Mesenchymal stem cells (MSCs) derived from bone marrow, umbilical cord blood, fat, etc., due to the short residence time in experimental animals, and Some studies have found that host immune rejection is induced, and MSCs cells have the risk of adverse reactions such as dysplasia, fibrosis, calcification, abnormal differentiation, formation of teratoma or other malignant proliferative tumors in animals, so the relevant data of clinical trials less
② Endothelial progenitor cells derived from bone marrow, peripheral blood, and umbilical cord blood: A large number of cell death was observed within a few hours after cell transplantation in animal models, and it has the risk of promoting tumor angiogenesis and vascular fibrosis. experiment report

Method used

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  • Application of human amniotic epithelial stem cells in preparation of medicine for treating acute kidney injury
  • Application of human amniotic epithelial stem cells in preparation of medicine for treating acute kidney injury
  • Application of human amniotic epithelial stem cells in preparation of medicine for treating acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Isolation and culture of primary human amniotic epithelial stem cells

[0061] 1 Source of human amniotic membrane

[0062] In order to avoid microbial contamination of the birth canal, fetal placenta was selected by caesarean section. Due to the stimulation of labor signals after term, the amniotic membrane will undergo apoptosis, so it is advisable to use premature fetal placenta (before 38 weeks). After the mother’s authorization and consent, the placental tissue of the healthy mother (HIV, syphilis, hepatitis A, hepatitis B, hepatitis C and other serological reactions were all negative) after caesarean section was taken, the placenta was cut with a cross knife, and the whole amniotic membrane was obtained by mechanical separation.

[0063] 2 Isolation of hAESCs (aseptic operation is required throughout the process)

[0064] The placenta of infants delivered by caesarean section before 39 weeks was obtained, the amniotic membrane was removed from the inne...

Embodiment 2

[0076] Example 2 Establishment of mouse acute kidney injury model

[0077] 1 Preoperative preparation: leave basic hematuria 7 days before operation. Fasting 6h before surgery.

[0078] 2 Bilateral ischemia-reperfusion acute kidney injury (ischemia-reperfusion injury, IRI) model:

[0079] 1) Turn on the temperature control system (36.9-37.1°C); soak surgical instruments in 75% alcohol; prepare surgical accessories, and set a timer.

[0080] 2) The mice were weighed and divided into random groups.

[0081] 3) Anesthesia: 2% sodium pentobarbital, 30-40 mg / kg, intraperitoneal injection.

[0082] 4) Operation: the back skin of the mouse was prepared, disinfected with povidone iodine, fixed on a heating pad in a prone position, and the rectal temperature was measured. Paravertebral longitudinal incision was made, and the skin and muscular layer were cut layer by layer. The kidney was bluntly picked out with a cotton swab, and the renal pedicle was clearly displayed; the renal p...

Embodiment 3

[0086] Embodiment 3 mouse model experiment grouping and processing

[0087] Group 1: Mice were randomly divided into sham operation group, control group (injection of human amniotic epithelial stem cell preservation solution immediately after operation) and treatment group (injection of human amniotic epithelial stem cell immediately after operation). 18-20 mice in each group.

[0088] 2 Treatment: In the sham operation group, surgical incision was made, the kidney was bluntly picked out with a cotton swab, and then placed back into the abdominal cavity without ischemia-reperfusion operation, and the skin was sutured. Both the control group and the hAECs treatment group completed bilateral renal ischemia-reperfusion surgery. The control group was injected with 100 μl of hAECs cell preservation solution immediately after the operation, and the hAECs treatment group was injected with 100 μl of cell preservation solution resuspended in the tail vein immediately after the operatio...

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PUM

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Abstract

The invention relates to application of human amniotic epithelial stem cells in treating acute kidney injury. The invention discloses a method for treating and / or improving acute kidney injury by using an effective dose of human amniotic epithelial stem cells or a cell preparation containing the human amniotic epithelial stem cells independently or in combination with other medicines, and animal experiments adopt an intravenous injection method to give the human amniotic epithelial stem cells to a mouse model. The dose range of each time of administration is 106-107 cells, and the acute kidney injury can be effectively relieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of human amniotic epithelial stem cells. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) has become one of the medical problems of global concern. It has been reported that there are about 13 million patients suffering from AKI every year in the world. AKI has high morbidity, many complications, high mortality, and huge medical consumption. Therefore, research on the pathogenesis and clinical diagnosis and treatment of AKI has become one of the new medical hotspots in recent years. [0003] AKI is a more serious problem in critically ill patients in the intensive care unit (ICU). In my country, 50% of AKI deaths are from ICU wards. In addition to complications such as uremia and hyperkalemia, common causes of AKI death in ICU patients include bleeding, infection, and failure of other organs. At present, there is no specific t...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61K9/10A61P13/12C12N5/071C12N5/073
CPCA61K35/50A61K9/0019A61K9/10C12N5/0605C12N5/0625A61P13/12A61K9/0024C12N2509/00C12N2509/10
Inventor 杨莉任奕飞陈颖李双玲徐大民郑茜子吕继成刘琴
Owner PEKING UNIV FIRST HOSPITAL
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