Establishment of mulberry leaf high performance liquid chromatography fingerprint and application of multi-component content determination in quality evaluation of mulberry leaf

A high-performance liquid chromatography and fingerprint technology, which is applied in the field of pharmaceutical analysis, can solve the problems of comprehensively evaluating the quality of mulberry leaves, and achieve good peak shape and resolution, good repeatability, and good stability

Active Publication Date: 2022-08-02
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the existing mulberry leaf quality detection method cannot comprehensively evaluate the quality of mulberry leaves from different sources as a whole, and to provide a method for establishing a mulberry leaf high performance liquid chromatography fingerprint. There are 31 common peaks in total, and 6 characteristic peaks have been identified. The separation of each chromatographic peak is good, and the multi-component quantitative determination of the 6 characteristic peaks can be carried out at the same time, avoiding the defects of single chemical component determination, and realizing the mulberry leaves. Overall Evaluation of Quality

Method used

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  • Establishment of mulberry leaf high performance liquid chromatography fingerprint and application of multi-component content determination in quality evaluation of mulberry leaf
  • Establishment of mulberry leaf high performance liquid chromatography fingerprint and application of multi-component content determination in quality evaluation of mulberry leaf
  • Establishment of mulberry leaf high performance liquid chromatography fingerprint and application of multi-component content determination in quality evaluation of mulberry leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1: the construction of mulberry leaf high performance liquid chromatography fingerprint

[0078] 1.1 Instruments

[0079] LC-20AD high performance liquid chromatograph, including LC-20AD pump, SIL-20A autosampler, CTO-20AC column oven, and SPD-M20A diode array detector (Shimadzu Co., Ltd., Japan), AB265-S Model Mettler electronic analytical balance (Swiss Mettler company), ultrapure water machine (American Millipore company), SB-5200D ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.).

[0080] 1.2 Drugs and Reagents

[0081] Methanol is chromatographically pure (SEPSRV company), formic acid is chromatographically pure (MERDA company), protocatechuic acid reference substance, neochlorogenic acid reference substance, chlorogenic acid reference substance, cryptochlorogenic acid reference substance, rutin reference substance (Sichuan Provincial Vikki Biotechnology Co., Ltd.), astragaloside reference substance (Chengdu Maide Biotechnology Co., ...

Embodiment 2

[0094] Example 2: Quality evaluation of mulberry leaves

[0095] 2.1 Quality evaluation of mulberry leaf HPLC fingerprint

[0096] Based on the fingerprints of the above 10 batches of samples, using the "Chinese Medicine Chromatographic Fingerprint Ultra-Information Characteristic Digital Evaluation System 4.0" software, the average value method was used to calculate the fingerprint at 254nm wavelength and generate the average control fingerprint (PRFP), calculate The macro-qualitative similarity (Sm), macro-quantitative similarity (Pm) and fingerprint homogeneity (α) of 10 batches of mulberry leaf samples were used to calculate the quality evaluation grade of 10 batches of mulberry leaf samples. The higher the grade, the worse the quality. As shown in Table 2, the quality of mulberry leaf samples from three sources S1, S3 and S9 was better.

[0097] Table 2 Fingerprint quality evaluation of 10 batches of mulberry leaves

[0098]

[0099]

[0100] 2.2 Quantitative dete...

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Abstract

The invention relates to the application of the establishment of a mulberry leaf high-performance liquid chromatographic fingerprint in combination with the determination of multi-component content in the quality evaluation of mulberry leaves. In the present invention, 70% methanol water is used as a solvent when establishing a mulberry leaf high-performance liquid chromatography fingerprint. , select ZORBAX SB‑C 18 As a chromatographic column, 0.05-0.15% formic acid methanol solution-0.05-0.15% formic acid aqueous solution was used as a mixed mobile phase for gradient elution, and the established fingerprint spectrum could realize the separation of the complex components of mulberry leaves, and could comprehensively reflect the mulberry leaves. Overall chemical information. The fingerprint spectrum established by the method of the invention has a total of 31 common peaks, and 6 characteristic peaks are identified, and the separation degree of each chromatographic peak is good, and the 6 characteristic peaks are quantitatively determined at the same time, so as to avoid the defect of single chemical composition measurement, and further To achieve a comprehensive evaluation of the quality of mulberry leaves.

Description

technical field [0001] The invention belongs to the field of drug analysis, and in particular relates to the application of the establishment of a mulberry leaf high-performance liquid chromatography fingerprint and the determination of multi-component content in the quality evaluation of mulberry leaf. Background technique [0002] Morus alba L., Morus alba L., is native to central and northern China, and is also cultivated in Korea, Japan, Mongolia, Europe and other places. The white mulberry (Morus alba), the black mulberry (M.nigra) and the red mulberry (M.rubra) are the three most famous species in the Morus genus. Mulberry Leaf is the dry leaf of mulberry, which is harvested after the first frost, removes impurities and sun-dried. Mulberry leaves are medicinal and edible, bitter in taste, sweet in taste, cold in nature, and belong to the lung and liver meridians. It was first recorded in the "Shen Nong's Herbal Classic". . Mulberry leaves contain flavonoids, alkaloi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8686G01N2030/324
Inventor 杜岩汤道权仲巧巧李丁香张染
Owner XUZHOU MEDICAL UNIV
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