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Citrus flavonoid protein powder and preparation method and application thereof

A technology of citrus and protein powder, applied in the application, food ingredients as emulsifier, oil-containing food ingredients, etc., can solve the problems of poor water solubility and absorption, and achieve the effect of expanding application fields and improving bioavailability

Pending Publication Date: 2021-08-20
重庆西南果品营养研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most citrus flavonoids have shown very good biological activity in vitro experiments, but because of their poor water solubility and absorbability, they directly affect the bioavailability of these active ingredients in organisms and cannot exert their due effects. Therefore, it is necessary to Using certain technical means to improve its bioavailability

Method used

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  • Citrus flavonoid protein powder and preparation method and application thereof
  • Citrus flavonoid protein powder and preparation method and application thereof
  • Citrus flavonoid protein powder and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0046] In the embodiment of the present invention, the HPLC analysis conditions are: chromatographic column: sunfire C18 liquid chromatography column (250mm×4.6, 5μm); mobile phase: acetonitrile-water (gradient: 0-15min, 40%-55% acetonitrile; 15min- 20min, 55%-70% acetonitrile; 20min-25min, 70%-80% acetonitrile; 25min-30min, 80%-40% acetonitrile; flow rate 1.0mL / min; column temperature: 35℃; detection wavelength; 326nm; sample injection Amount 10 μ L.The preparation of embodiment 1 soluble citrus flavonoid active protein powder

[0047] (1) Citrus seed oil extraction

[0048] 1) Raw material pretreatment: Put the tangerine seeds after removing impurities and cleaning into an oven for drying, crush them with a multifunctional pulverizer, and pass through a 40-mesh sieve.

[0049] 2) Add n-hexane according to the ratio of solid to liquid 1:7, and extract 2 times with ultrasonic power 150W, frequency 80Hz, ultrasonic time 30min.

[0050] 3) Collect the extract and carry out cen...

Embodiment 2

[0059] Tangeretin content in the protein powder of embodiment 2

[0060] (1) Establish a standard curve

[0061] Dissolve standard orange peelin in methanol, prepare 6 groups of standard solutions with different concentrations (0.005mg / ml, 0.01mg / ml, 0.05mg / ml, 0.1mg / ml, 0.2mg / ml, 0.5mg / ml) HPLC analysis, analysis result is as shown in table 1 below, wherein a group of chromatograms are as follows figure 1 shown.

[0062] Table 1 HPLC analysis of citrus flavonoid active protein powder data sheet

[0063]

[0064] From the analysis of Table 1, it is found that the relationship between concentration and peak area is shown in Table 2, and linear regression is performed as figure 2 shown.

[0065] Table 2 Tangeretin concentration and peak area relationship

[0066] Concentration (mg / ml) Peak area 0.005 188326.5 0.01 494970.8 0.05 2094326 0.1 4406436 0.2 8565816 0.5 20865049

[0067] (2) Analysis of Tangeretin Content

[0068...

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Abstract

The invention belongs to the field of food / health product preparation, and particularly relates to citrus flavonoid protein powder abd a preparation method and application thereof. The citrus flavonoid active protein powder is characterized in that the preparation method of the citrus flavonoid active protein powder comprises the following steps of: (1) adding hesperetin into citrus seed oil according to a concentration ratio of 10 to 30mg / g to obtain an oil phase; dissolving concentrated whey protein and a composite wall material into water to obtain a water phase; (2) mixing the oil phase and the water phase to obtain an emulsion; and (3) drying the emulsion to obtain the citrus flavonoid active protein powder, wherein the composite wall material comprises maltodextrin and sodium caseinate, and the weight ratio of the maltodextrin to the sodium caseinate is (1 to 2): 5. The citrus flavonoid active protein powder solves the problem of low bioavailability caused by poor solubility of citrus flavonoid, and the concentrated whey protein is added into the product, so that the citrus flavonoid active protein powder is a good nutritional health product, and the application field of the hesperetin in an aqueous solution is expanded.

Description

technical field [0001] The invention belongs to the field of food / health product production, and in particular relates to a citrus flavonoid protein powder, its preparation method and application. [0002] technical background [0003] Citrus is the largest fruit in China and even in the world. At present, citrus fruit is widely used in fresh food and other industrial agricultural product processing, such as canned orange petals, juice, etc., so a large number of by-products - citrus seeds will be produced accordingly. my country's deep processing technology is weak, equipment is outdated, and a large number of citrus seeds are discarded, which not only leads to waste of resources but also causes environmental pollution. Citrus seed oil is rich in unsaturated fatty acids, especially oleic acid and linoleic acid, which are higher than other oils. Therefore, citrus seed oil has high nutritional value and is a high-quality health-care vegetable oil. In addition to being rich in...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/19A23P10/47
CPCA23L33/105A23L33/19A23P10/47A23V2002/00A23V2200/30A23V2250/21164A23V2250/18A23V2250/54252A23V2250/5114A23V2250/61A23V2250/16A23V2250/1618A23V2250/1628A23V2200/222A23V2250/192A23V2300/10
Inventor 周志钦刘威
Owner 重庆西南果品营养研究院
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