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Preparation method of fibroin-cellulose nano composite mask

A silk protein and nano-composite technology, which can be used in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulations, etc., and can solve problems such as poor toughness

Inactive Publication Date: 2021-08-03
哈尔滨辛格尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacterial cellulose has certain disadvantages, and its own toughness is poor, so it is necessary to further prepare functional composite membranes

Method used

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  • Preparation method of fibroin-cellulose nano composite mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of silk protein-cellulose nanocomposite facial mask, the steps are as follows:

[0018] (1) Culture medium preparation: ultrafinely pulverize defatted corn germ and sieve; dissolve defatted corn germ powder in sodium bicarbonate solution, add alkaline protease (Protext 6L) for enzymatic hydrolysis, and inactivate enzyme; add glucose, yeast soak powder; adjust the pH to 4.5; centrifuge; filter; high temperature instantaneous sterilization. (2) Inoculation of Kombucha; (3) Fermentation; (4) NaOH solution dissolves bacterial cellulose to prepare pure cellulose film, and high-voltage electrostatic spraying method applies silk protein solution on the pure cellulose film to prepare silk protein-cellulose nano Composite mask.

[0019] Preferably, the liquid culture medium is prepared by: superfinely pulverizing defatted corn germ, passing through a 600-mesh sieve; dissolving defatted corn germ powder in sodium bicarbonate solution, wherein the mass ratio ...

Embodiment 2

[0024] A preparation method of silk protein-cellulose nanocomposite facial mask, the steps are as follows:

[0025] (1) Culture medium preparation: ultrafinely pulverize defatted corn germ and sieve; dissolve defatted corn germ powder in sodium bicarbonate solution, add alkaline protease (Protext 6L) for enzymatic hydrolysis, and inactivate enzyme; add glucose, yeast soak powder; adjust the pH to 5.0; centrifuge; filter; high temperature instantaneous sterilization. (2) Inoculation of Kombucha; (3) Fermentation; (4) NaOH solution dissolves bacterial cellulose to prepare pure cellulose film, and high-voltage electrostatic spraying method applies silk protein solution on the pure cellulose film to prepare silk protein-cellulose nano Composite mask.

[0026] Preferably, the liquid culture medium is prepared by: superfinely pulverizing defatted corn germ, passing through a 700-mesh sieve; dissolving defatted corn germ powder in sodium bicarbonate solution, wherein the mass ratio ...

Embodiment 3

[0031] A preparation method of silk protein-cellulose nanocomposite facial mask, the steps are as follows:

[0032] (1) Culture medium preparation: ultrafinely pulverize defatted corn germ and sieve; dissolve defatted corn germ powder in sodium bicarbonate solution, add alkaline protease (Protext 6L) for enzymatic hydrolysis, and inactivate enzyme; add glucose, yeast soak powder; adjust the pH to 5.5; centrifuge; filter; high temperature instantaneous sterilization. (2) Inoculation of Kombucha; (3) Fermentation; (4) NaOH solution dissolves bacterial cellulose to prepare pure cellulose film, and high-voltage electrostatic spraying method applies silk protein solution on the pure cellulose film to prepare silk protein-cellulose nano Composite mask.

[0033]Preferably, the liquid culture medium is prepared by: superfinely pulverizing defatted corn germ, passing through a 800-mesh sieve; dissolving defatted corn germ powder in sodium bicarbonate solution, wherein the mass ratio o...

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Abstract

The invention relates to a preparation method of a fibroin-cellulose nano composite mask. The preparation method comprises the following steps: (1) preparing a culture medium: performing superfine grinding on degreased corn germs, and sieving; dissolving degreased corn germ powder in a sodium bicarbonate solution, adding alkaline protease (Protext 6L) for enzymolysis, and performing enzyme deactivation; adding glucose and yeast extract powder; adjusting the pH value to 4.5 to 5.5; performing centrifugation; filtering; and performing high-temperature instantaneous sterilization. (2) inoculating kombucha; (3) fermenting; and (4) dissolving the bacterial cellulose by using a NaOH solution to prepare a pure cellulose membrane, and coating the pure cellulose membrane with the fibroin solution by using a high-voltage electrostatic spraying method to prepare the fibroin-cellulose nano composite mask.

Description

technical field [0001] The invention relates to the technical field of bacterial cellulose membrane fermentation and compounding, in particular to a preparation method of a silk protein-cellulose nanocomposite facial mask. Background technique [0002] Corn germ meal is the residue after corn germ is extracted from corn oil. It is the main by-product of corn oil factory and is a high-nutrient substance mainly composed of corn fiber and protein. Among them, the mass fraction of crude protein is generally 23% to 25%, and the biological potency PER value is close to the WHO / FAO recommended value, equivalent to soybean casein, higher than rice and refined flour, and is a high-quality protein source. After the corn germ is fried in oil, except for the reduction of oil content, other nutrients are basically retained in the germ meal. However, domestic factories generally treat corn germ meal as feed, and do not give full play to its potential biological value. [0003] Bacterial ...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61K8/73A61K8/99A61K8/64A61K8/02A61Q19/00
CPCA61K8/0212A61K8/64A61K8/731A61K8/99A61K2800/56A61K2800/85A61Q19/00A61K8/9794
Inventor 曹亮韩天翔俞红梅夏秀芳
Owner 哈尔滨辛格尔生物科技有限公司
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