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Carbon dots for detecting progesterone and progesterone detection method

A technology of progesterone and carbon dots, which is applied in the field of nanomaterials, can solve the problems of long sample pretreatment time, long running time, and low sensitivity, and achieve the effects of fast detection speed, low detection limit, and broad application prospects

Active Publication Date: 2021-06-25
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Methods for detecting progesterone include chromatographic analysis, colorimetry, electrochemical methods, etc. However, these methods have certain disadvantages, such as long sample pretreatment time, expensive reagents, long running time, and low sensitivity

Method used

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  • Carbon dots for detecting progesterone and progesterone detection method
  • Carbon dots for detecting progesterone and progesterone detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This embodiment provides a carbon dot for detecting progesterone, which is prepared by the following steps:

[0042] 1) 0.5g curcumin is put into a beaker, heated at 180° C. for 6 hours in a reaction kettle to obtain a brown intermediate product;

[0043] 2) After the intermediate product was naturally cooled to room temperature, it was dissolved in ethanol and centrifuged at 5000rpm for 30 minutes;

[0044] 3) The obtained centrifugate is dialyzed with a dialysis bag to remove unreacted small molecules, and the dialyzed product is then freeze-dried to obtain carbon dots.

[0045] refer to figure 1 , figure 1 A is the absorption spectrum of the carbon dot that embodiment 1 makes, figure 1 B is the emission spectrum of carbon dots under different wavelengths of excitation light, figure 1 In B, at the position of the arrow, the wavelength increases sequentially from top to bottom, that is, the wavelength of the curve from top to bottom is 360, 380, 400, 420, 440, 460,...

Embodiment 2

[0047] This implementation provides a detection method for progesterone, which uses the carbon dots prepared in Example 1 to detect, and the specific detection steps include:

[0048] 1) Construction of a standard curve for progesterone detection:

[0049] Mix n parts of progesterone solutions with a certain concentration gradient with a volume of v1 each with a carbon dot solution with a volume of v2 and a concentration of c, and let stand;

[0050] Test the fluorescence intensity values ​​of the mixed solutions at the excitation wavelength of 410nm for the n parts of the mixed solutions obtained;

[0051] Taking the progesterone concentration as the abscissa and the fluorescence intensity as the ordinate, a standard curve for progesterone detection is obtained by fitting;

[0052] 2) detect the progesterone concentration in the solution to be tested:

[0053] Take the solution to be tested with a volume of v1 and mix it with a carbon dot solution with a volume of v2 and a ...

Embodiment 3

[0067] In this embodiment, an interference experiment of carbon dots for progesterone concentration detection is carried out.

[0068] In this example, the interfering substances used include Cu 2+ ,Fe 3+ ,K + , Na + ,Pb 2+ ,Hg 2+ ,Al 3+ ,Mn 2+ , Ca 2+ ,AA,Cys,H 2 o 2 , the concentration is 120μmol / L. The experimental method is: mix carbon dots with 120 μmol / L interfering substance solution, and at the same time mix the same concentration of carbon dots with progesterone with a concentration of 120 μmol / L as a comparison, let stand for 3 minutes; then test at the excitation wavelength of 350nm The fluorescence intensity values ​​of the two mixed solutions; the above experiments were carried out separately for all interfering substances. refer to figure 2 D, for the experimental results, wherein P4 represents progesterone, to see that only progesterone has an enhanced effect on the fluorescence of the carbon dot solution, while other interfering substances have no ...

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Abstract

The invention discloses carbon dots for detecting progesterone and a progesterone detection method. The carbon dots are prepared through the following steps of: (1) putting curcumin into a container, and heating to obtain an intermediate product; (2) cooling the intermediate product to room temperature, dissolving the intermediate product in ethanol, and centrifuging; and (3) dialyzing the obtained centrifugate by using a dialysis bag, and freeze-drying the dialyzed product to obtain the carbon dots. The prepared carbon dots are good in stability, high in luminous intensity, simple to prepare and low in cost, and progesterone can specifically enhance fluorescence of the carbon dots. The progesterone detection method established by the carbon dots has the characteristics of low detection limit, accurate and reliable detection and high detection speed, can provide sensitive and accurate detection results, and has a wide application prospect in the field of progesterone specific detection.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a carbon dot for detecting progesterone and a progesterone detection method. Background technique [0002] Progesterone (P4) is a steroid hormone secreted by the ovary, which plays a vital role in maintaining and regulating physiological processes such as female menstrual cycle, embryonic development, pregnancy and breast development, and is also used in human medicine and clinical treatment is also widely used. However, the abuse of progesterone may cause adverse side effects such as weakness, depression, breast discomfort, certain potential carcinogenic effects, etc. Sensitivity analysis of progesterone monitoring has high requirements in clinical medicine, animal husbandry and environmental protection. Therefore, it is of great significance to detect progesterone efficiently and sensitively. [0003] Methods for detecting progesterone include chromatographic analysis, colorimet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/6428
Inventor 昝明辉梅茜董文飞安帅吴再辉刘莹葛明锋李力
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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