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High performance liquid chromatography analysis method simultaneously adapting to determination of 24 antiepileptic drugs in human plasma

A high-performance liquid chromatography and anti-epileptic drug technology, applied in the field of high-performance liquid chromatography analysis, can solve problems such as affecting decision-making time, inconvenient monitoring of anti-epileptic drugs, and affecting the quality of epilepsy treatment.

Active Publication Date: 2021-05-04
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, more than 20 varieties have been put into clinical use. Clinical pharmacy workers in hospitals may face epilepsy patients who take different drugs every day. If each drug or several drugs need to establish an HPLC analysis method, it will inevitably give anti Therapeutic drug monitoring of epilepsy drugs brings inconvenience to work, and may also affect the decision-making time for individualized medication and clinicians to adjust doses, thereby affecting the quality of epilepsy treatment

Method used

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  • High performance liquid chromatography analysis method simultaneously adapting to determination of 24 antiepileptic drugs in human plasma
  • High performance liquid chromatography analysis method simultaneously adapting to determination of 24 antiepileptic drugs in human plasma
  • High performance liquid chromatography analysis method simultaneously adapting to determination of 24 antiepileptic drugs in human plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Chromatographic conditions

[0074] Using Eclipse Plus C 18 (150×4.6mm, 3.5μm) chromatographic column, use acetonitrile (A)-5mM potassium dihydrogen phosphate solution (B) as the mixed mobile phase for gradient elution, and perform gradient elution according to the following process: 0min, 10%A; 2min, 10%A; 3min, 28%A; 7.5min, 28%A; 8min, 35%A; 8.5min, 45%A; 9.5min, 55%A; 11min, 60%A; 11.5min, 65% A; 20min, 65%A, see Table 1 for details; column temperature is 35°C, detection wavelength is 210nm.

[0075] 2. Experimental process

[0076] (1) Preparation of reference substance:

[0077] For the first group of antiepileptic drugs, 15 antiepileptic drugs (piracetam; levetiracetam; ethosuximide; primidone; lacosamide; suthiazide; zonisamide; brivaracetam ; phenobarbital; oxcarbazepine; ricarbazepine; phenytoin; clonazepam; diazepam; Sripanto) are respectively configured into 2.0mg / mL mother solution by using 50% acetonitrile-water solution. Take 750 μL of each of the...

Embodiment 2

[0082] 1. Chromatographic conditions

[0083] Using Eclipse Plus C 18 (150×4.6mm, 3.5μm) chromatographic column, use acetonitrile (A)-5mM potassium dihydrogen phosphate solution (B) as the mixed mobile phase for gradient elution, and perform gradient elution according to the following process: 0min, 20%A; 2min, 20%A; 5min, 30%A; 8min, 60%A; 10min, 60%A; 13min, 60%A; 15min, 20%A, see Table 2 for details; , and the injection volume was 10 μL.

[0084] 2. Experimental process

[0085] (1) Preparation of reference substance:

[0086] For the second group of antiepileptic drugs, the preparation process of the reference substance is as follows: 6 kinds of antiepileptic drugs (lamotrigine; felbamate; rufinamide; saigabine; carbamazepine; perampanel) were all used 50% acetonitrile-water solution was respectively configured into 1.0 mg / mL mother liquor.

[0087] Take 600 μL of each of the mother solutions of the above six antiepileptic drugs, add 400 μL of acetonitrile to the inte...

Embodiment 3

[0092] 1. Chromatographic conditions

[0093] Using Eclipse Plus C 18 (150×4.6mm, 3.5μm) chromatographic column, use acetonitrile (A)-5mM potassium dihydrogen phosphate solution (B) as the mixed mobile phase for gradient elution, and perform gradient elution according to the following process: 0min, 20%A; 2min, 20%A; 3min, 28%A; 7.5min, 28%A; 8min, 35%A; 8.5min, 45%A; 9.5min, 55%A; 11min, 60%A; 11.5min, 65% A; 20 min, 65% A, see Table 3 for details; the column temperature is 35°C, the detection wavelength is 210nm, and the injection volume is 10 μL.

[0094] 2. Experimental process

[0095] (1) Preparation of reference substance:

[0096] For the third group of antiepileptic drugs, the preparation process of the reference substance is as follows: 3 kinds of antiepileptic drugs (vigabatrin; pregabalin; gabapentin) were prepared into 1.0 mg / mL mother solution with 50% acetonitrile-water solution.

[0097] Take 600 μL of each of the mother solutions of the above three antiepi...

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Abstract

The invention relates to a high performance liquid chromatography analysis method simultaneously adapting to determination of 24 antiepileptic drugs in human plasma. The 24 antiepileptic drugs are grouped according to the types, chromatographic analysis is carried out according to different gradient elution conditions and under basically same chromatographic conditions, the 24 antiepileptic drugs in the human plasma can be simultaneously detected, and an ultraviolet derivatization reagent is added into a drug needing to be derivatized for pre-column derivatization, so that the anti-epileptic medicines without ultraviolet absorption can be detected, the separation efficiency is high, and the selectivity is better.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a high-performance liquid chromatography analysis method simultaneously adapted to the determination of 24 kinds of antiepileptic drugs in human plasma, wherein the 24 kinds of antiepileptic drugs are respectively: 1. piracetam; 2. Levetiracetam; 3. Ethosuximide; 4. Primidone; 5. Lacosamide; 6. Suthiazide; 7. Zonisamide; 8. Brivaracetam; 9. Phenobarbital ;10. Oxcarbazepine; 11. Licarbazepine; 12. Phenytoin; 13. Clonazepam; 14. Diazepam; 15. Slipanto; 16. Lamotrigine; 17. Felamine Esters; 18. Rufinamide; 19. Cygabine; 20. Carbamazepine; 21. Perampanel; 22. Vigabatrin; 23. Pregabalin; 24. Gabapentin. Background technique [0002] Epilepsy is a common disease of the nervous system. It is a chronic disease in which the sudden abnormal discharge of brain neurons leads to transient brain dysfunction, affecting about 70 million people worldwide. In China, epilepsy ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 汤道权赵琳琳杜岩王陈祥郭梦喆季帅
Owner XUZHOU MEDICAL UNIV
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