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Production of high-quantum-yield sulfur quantum dots and method for determining ascorbic acid thereof

A technology of ascorbic acid and sulfur quantum, applied in analytical chemistry and nanometer fields, can solve the problems of harsh experimental conditions, unfavorable rapid analysis, complicated and tedious steps, etc., and achieve simple and easy steps, good fluorescence performance and photostability, and good selectivity. Effect

Pending Publication Date: 2020-12-11
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, some methods require relatively harsh experimental conditions and high operating techniques, while some methods are complicated and cumbersome, which is not conducive to the requirements of rapid analysis.

Method used

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  • Production of high-quantum-yield sulfur quantum dots and method for determining ascorbic acid thereof
  • Production of high-quantum-yield sulfur quantum dots and method for determining ascorbic acid thereof
  • Production of high-quantum-yield sulfur quantum dots and method for determining ascorbic acid thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for high quantum yield sulfur quantum dots, comprising the following steps:

[0032] Accurately weigh 0.175 g of sublimed sulfur powder, add 1.2 mL of polyethylene glycol-400, and add 0.8 g of NaOH. After mixing evenly, ultrasonic microwave method at 70 ℃ for 1 h, add 5 mL of 12 wt% H 2 o 2 And continue to microwave for 1 h, centrifuge the obtained product sulfur quantum dots at 7000 rpm for 10 min, take the supernatant and dialyze it with a 100-500 D dialysis bag for 12 h to purify, seal and store to obtain the sulfur quantum dot solution.

[0033] The morphology of sulfur quantum dots was analyzed by transmission electron microscopy, as shown in figure 1 As shown, it can be seen that the prepared sulfur quantum dots are spherical, the particle size distribution is uniform, and the average diameter is 2.22 ± 0.6 nm.

[0034] Such as figure 2 As shown, the sulfur quantum dots prepared by the present invention have a constant maximum emission wav...

Embodiment 2

[0036]Selection of Ce(IV) concentration in the composite solution of sulfur quantum dots / Ce(IV) fluorescent probe: Sodium citrate buffer (10 mM, 1325 μL, pH= 3), the sulfur quantum dots prepared in Example 1 (75 μL) and different concentrations of cerium sulfate solutions (50 μL) were successively added to 2 ml EP tubes, reacted at 37 °C for 10 minutes, and measured the fluorescence intensity at 453 nm of the above system. Such as image 3 As shown, after adding different concentrations of Ce(IV) to the solution, the fluorescence of sulfur quantum dots will be quenched sharply, and with the increase of Ce(IV) ion concentration, sulfur in the sodium citrate system at pH=3 The fluorescence of quantum dots is gradually quenched. When the concentration of Ce(IV) reaches 20 μM, the fluorescence quenching of sulfur quantum dots reaches 60%. As the concentration of Ce(IV) continues to increase, the fluorescence of sulfur quantum dots is further quenched. , but the quenching efficien...

Embodiment 3

[0038] Application of the sulfur quantum dot solution obtained in Example 1 in the detection of ascorbic acid.

[0039] The detection method is as follows: sodium citrate buffer (10 mM, 1325 μL, pH=3), the sulfur quantum dots prepared in Example 1 (75 μL), different concentrations of ascorbic acid solution (50 μL) and 0.6 mM The cerium sulfate solution (50 μL) was successively added into 2 ml EP tubes and reacted at 37°C for 10 minutes. Sulfur quantum dot solution and Ce(IV) solution are mixed (buffer: anhydrous citric acid-sodium citrate solution at pH=3) to obtain sulfur quantum dot / Ce(IV) fluorescent probe composite solution, fluorescence quenching, test Fluorescence intensity at the excitation wavelength of 364 nm, and then ascorbic acid solutions with different final concentrations were added to the sulfur quantum dot / Ce(IV) fluorescent probe composite solution at the same time, and the fluorescence intensity of each system at the excitation wavelength of 364 nm was teste...

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Abstract

The invention discloses production of high-quantum-yield sulfur quantum dots and a method for determining an ascorbic acid thereof. The high-quantum-yield sulfur quantum dots disclosed by the invention are produced by taking polyethylene glycol-400 as a cosolvent and a passivating agent and adopting an ultrasonic microwave-assisted method under an alkaline condition. A fluorescence quantum yield of the sulfur quantum dots produced by the method is 58.65%. The high-quantum-yield sulfur quantum dots are used as a fluorescent probe, and based on a quenching effect of Ce (IV) on the fluorescence and redox reaction of Ce (IV) and the ascorbic acid, when the Ce (IV), the ascorbic acid and sulfur quantum dots coexist, fluorescence quenching of the sulfur quantum dots is inhibited so that quantitative detection of the ascorbic acid is realized. The method is low in cost, high in sensitivity, good in linear relation, easy and convenient to operate, good in selectivity and capable of being usedfor measuring the ascorbic acid in an actual sample, and therefore the method has good clinical application prospects.

Description

technical field [0001] The invention relates to the preparation of sulfur quantum dots with high quantum yield and a method for measuring ascorbic acid, belonging to the field of analytical chemistry and nanotechnology. Background technique [0002] Sulfur Quantum Dots (Sulfur Quantum Dots) is a new type of single-element fluorescent quantum dot material, because of its tunable fluorescence emission, good photostability, large Stokes shift and good biocompatibility Such excellent fluorescent properties, low preparation cost, unique antibacterial properties, etc., are expected to gradually replace heavy metal quantum dots and organic dyes, and are widely used in catalysis, analysis, biology, medicine and other fields. So far, researchers' research on sulfur quantum dots is still in its infancy, because of its complex preparation process, single function, and low quantum yield, which greatly limits its application. [0003] L-Ascorbic Acid (Ascorbic Acid), also known as Vitam...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/56C01B17/027B82Y20/00
CPCG01N21/6428C09K11/56C01B17/027B82Y20/00G01N2021/6432
Inventor 彭花萍盛依伦黄种南魏朝国陈伟
Owner FUJIAN MEDICAL UNIV
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