Identification method of Chinese wolfberry color-related metabolite
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An identification method and metabolite technology, which is applied in the field of identification of metabolites related to the color of wolfberry, can solve the problem that there is no relevant research on the depth of wolfberry color, and achieve the effect of improving accuracy
Pending Publication Date: 2020-11-13
WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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[0003] At present, there are many studies on plant anthocyanins. Studies have shown that anthocyanins are also key metabolites that affect fruit color. Different anthocyanin contents and types are the reasons for the color differences of white, red and black fruits. There is no relevant research on the cause of the depth of the color
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Embodiment 1
[0031] Lycium barbarum pretreatment experiment
[0032] Optimization of extraction solvent, optimization of solid-liquid ratio, optimization of extraction time, etc., by comparing different pretreatment methods to extract more anthocyanins from Lycium barbarum, and conducting cross experiments among the following variables to select the optimal extraction conditions.
[0033] Table 1 different volume percent methanol solvent
[0034]
[0035] Table 2 Different material-to-liquid ratios
[0036] Different ratio of solid to liquid (g / ml) 1:10 1:20 1:30 1:40 1:50
[0037] Table 3 Different ultrasonic time
[0038] Different ultrasonic time (min) 5 10 20 30 60
[0039] Table 4 Different ultrasonic temperatures
[0040] Different ultrasonic temperature(℃) 20 30 40 50 60
[0041] The optimization steps are:
[0042] 1.1 Methanol was used as the extractant, and the extraction effect of petunidin compounds with differen...
Embodiment 2
[0050] Liquid phase and mass spectrometry conditions of Lycium barbarum anthocyanins
[0051] The data acquisition instrument system mainly includes Ultra Performance Liquid Chromatography (UPLC) and Tandem mass spectrometry (MS / MS).
[0054] (2) mobile phase: A is ultrapure water, B is acetonitrile, all adds the acetic acid of 0.1% (volume percentage);
[0055] (3) The flow rate is 0.3ml / min; the column temperature is 40°C; the injection volume is 2μl.
[0056] Mobile phase step elution according to the following volume percentages:
[0057] Table 7 Elution Gradient
[0058]
[0059] Mass spectrometry conditions:
[0060] Table 8 Mass Spectrometry Parameters
[0061] ion mode positive ion scan air curtain pressure 40psi ion spray voltage 5500V temperature 550℃ Ion source gas 1 50psi Ion source gas 2 60psi ...
Embodiment 3
[0063] Seventeen Lycium germplasms with different fruit colors were selected, including 8 Lycium barbarum with dark fruit color and 9 Lycium barbarum with light fruit color.
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Abstract
The invention provides an identification method of a Chinese wolfberry color-related metabolite, which comprises the following steps of: pretreating Chinese wolfberry fruit samples of Chinese wolfberry varieties of different colors, extracting anthocyanin, and carrying out qualitative and quantitative detection on the anthocyanin; analyzing the anthocyanin detection data, and positioning chromatographic ion peaks to obtain a Chinese wolfberry anthocyanin database; and performing quantitative analysis, and screening to obtain a specific metabolite about the color depth of the Chinese wolfberryfruits. The method disclosed by the invention has the beneficial effects that a basis is provided for subsequent deeper research on specific substances about the color depth of the Chinese wolfberry,and meanwhile, convenience is provided for subsequent metabolite research and breeding research; by exploring ultra-high performance liquid chromatography-mass spectrometry test conditions, the anthocyanin detection accuracy is improved; and an identification method of a Chinese wolfberry color-related metabolite is provided, an anthocyanin database is constructed, a material basis is provided forlater research, and a research thought is also provided for identifying plant color-related metabolites in the future.
Description
technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to an identification method for color-related metabolites of wolfberry. Background technique [0002] Anthocyanin is a kind of natural food coloring, safe and non-toxic, rich in resources, and has certain physiological health functions, such as removing free radicals in the body, anti-tumor, anti-cancer, protecting the liver, preventing diabetes, etc. After research, anthocyanins are derivatives with the typical structure of flavonoids, 2-phenylbenzopyran cationic structure. Foods mainly contain anthocyanins, namely cyanidin, morning glory pigment, delphinium pigment, peony pigment, geranium pigment and mallow pigment. [0003] At present, there are many studies on plant anthocyanins. Studies have shown that anthocyanins are also key metabolites that affect fruit color. Different anthocyanin contents and types are the reasons for the color differences of white, red a...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 安巍赵建华尹跃李浩霞陈曦胡丽娟黄稳娥曹有龙
Owner WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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