Application of microalgae carbon sequestration enhancer and culture medium containing enhancer
A culture medium and enhancer technology, applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., can solve the problems of limited physical and chemical properties of culture fluid, low concentration of total inorganic carbon, and growth limitation of microalgae, and achieve no Effects of poisoning, improving carbon fixation efficiency, and improving conversion efficiency
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Embodiment 1
[0027] A columnar glass photoreactor (height 50cm, inner diameter 6cm) is used indoors for microalgae cultivation, and aeration stones (aperture 30-60μm) commonly used in this field are used for ventilation, and the light intensity provided by 8 fluorescent tubes is 100μmol / m 2 / s of 12h:12h (light:dark) lighting.
[0028] The cultured algae is Chlorella vulgaris, which comes from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences, numbered FACHB-8. In the control group, BG11 was used as medium to culture Chlorella, and the culture volume was 1L. 0.5% methanol by volume was added to the medium of the methanol experimental group; polyethylene glycol dimethyl ether was added to the medium of the NHD experimental group, with a final concentration of 5 mmol / L; propylene carbonate was added to the medium of the PC experimental group, and finally The concentration was 5mmol / L; N-methylpyrrolidone was added to the culture medium of the NMP experi...
Embodiment 2
[0037] A columnar glass photobioreactor was used to cultivate marine Nannochloropsis indoors. The algae species came from the Freshwater Algae Species Bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 926. The control group was cultured in f / 2 medium, and the experimental group was cultured in f / 2 medium supplemented with PC. The final concentration of PC was 5 mmol / L. Others are the same as in Example 1, the inoculation concentration of microalgae cells is 0.05g / L.
[0038] During the culture period, the average total inorganic carbon source concentration in the culture solution of the control group was 4.95mmol / L. The average total inorganic carbon source concentration in the culture medium of the methanol experimental group was 6.46mmol / L, which was 62.2% higher than that of the control group. The average biological carbon fixation efficiency of the control group was 121.2 mg / L / d after 8 days of batch culture, and the average biological carbon fixa...
Embodiment 3
[0040] Carry out an open track pool culture experiment. The runway pool has a circumference of 70 meters and a width of 3 meters. The algal species is Scenedesmus dimorphus, which comes from the freshwater algae species bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 496. Cultivate Scenedesmus dimorphus with BG11 medium, the depth of the culture medium is 20 cm; add PC to the final concentration of 5 mmol / L when preparing the medium; rely on the natural evaporation of water to maintain the temperature of the culture medium not exceeding 30°C; the flow of the culture medium Driven by the stirring impeller, the carbon replenishment adopts automatic control (there are related control devices and technology published documents, such as patents CN200410009360.4 and CN201210138598.1). The raceway pond culture in the medium without adding PC was used as the control group. The results showed that the average total inorganic carbon concentration in the cult...
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