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Preparation method of wound repair material with cell growth regulation and control function

A technology for wound repair and cell growth, which is applied in filament/thread forming, medical science, non-woven fabrics, etc. It can solve the problems that it is difficult to meet the design needs of wound repair materials, and cannot achieve precise regulation of cell growth genes. Conducive to wound repair and regeneration, isolation of bacterial invasion and infection, good tensile strength

Active Publication Date: 2020-10-09
GUANGDONG POLYTECHNIC OF IND & COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to allow cells to adhere, grow and differentiate effectively on the surface of the material, the hydrophilicity of the surface of the scaffold material and the ability to adhere to cells are generally changed by various physical, chemical and biological modification methods, or by adding growth factors , such as recombinant human epidermal growth factor (hEGF), recombinant human basic fibroblast growth factor (hbFGF), recombinant human keratinous growth factor (hKGF) and recombinant human vascular growth factor (hVGF) to promote cell proliferation and differentiation , it is difficult to meet the design requirements of wound repair materials simply by adding growth factors, and it is impossible to achieve precise regulation of cell growth genes. Therefore, it is necessary to develop a new method of cell growth regulation

Method used

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  • Preparation method of wound repair material with cell growth regulation and control function
  • Preparation method of wound repair material with cell growth regulation and control function
  • Preparation method of wound repair material with cell growth regulation and control function

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A preparation method of a wound repair material with cell growth regulation function, comprising the following steps:

[0036] S1. In the glass container, add 0.4g polycaprolactone (the number average molecular weight is 50000) and 0.2g nanometer bioactive glass powder (CaO-P 2 o 5 -SiO 2 system, SiO 2 59wt%, P 2 o 5 5wt%, CaO 36wt%) in 5ml hexafluoroisopropanol, to obtain a solution with a mass volume ratio concentration of 0.12g / ml, the solution was magnetically stirred at room temperature for 12h to dissolve completely, and was prepared as an electrospinning precursor solution;

[0037] S2. Inhale the electrospinning precursor solution configured in step S1 into the syringe fixed on the propulsion pump to carry out the electrospinning process. The propulsion rate of the propulsion pump is 1.4 ml / hour and the flow rate of the spinning solution is kept stable. The spinning nozzle is a diameter of It is a flat needle of 0.4 mm, connected to the positive electrode...

Embodiment 2

[0044] A preparation method of a wound repair material with cell growth regulation function, comprising the following steps:

[0045] S1. In the glass container, add 0.6g polyvinyl alcohol and 0.3g nano bioactive glass powder (CaO-P 2 o 5 -SiO 2 system, SiO 2 50wt%, P 2 o 5 20wt%, CaO 30wt%) into 6ml of acetone, to obtain a solution with a mass volume ratio concentration of 0.16g / ml, the solution was magnetically stirred at room temperature for 12h to dissolve completely, and was prepared as an electrospinning precursor solution;

[0046] S2. Inhale the electrospinning precursor solution configured in step S1 into the syringe fixed on the propulsion pump to carry out the electrospinning process. The propulsion rate of the propulsion pump is 1.5 ml / hour and the flow rate of the spinning solution is kept stable. The spinning nozzle is a diameter of It is a flat needle of 0.4 mm, connected to the positive pole of 20 kV high-voltage DC power supply, the distance between the s...

Embodiment 3

[0053] A preparation method of a wound repair material with cell growth regulation function, comprising the following steps:

[0054] S1. In the glass container, add 1.0g polylactic acid-glycolic acid copolymer (the number average molecular weight is 50000) and 0.5g nanometer bioactive glass powder (CaO-P 2 o 5 -SiO 2 system, SiO 2 60wt%, P 2 o 5 14wt%, CaO 26wt%) into 10ml tetrahydrofuran, to obtain a solution with a mass volume ratio concentration of 0.20g / ml, the solution was magnetically stirred at room temperature for 12h to dissolve completely, and was prepared as an electrospinning precursor solution;

[0055] S2. Inhale the electrospinning precursor solution configured in step S1 into the syringe fixed on the propulsion pump to carry out the electrospinning process. The propulsion rate of the propulsion pump is 1.5 ml / hour and the flow rate of the spinning solution is kept stable. The spinning nozzle is a diameter of It is a flat needle of 0.4 mm, connected to the...

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Abstract

The invention relates to the field of wound repair materials, and provides a preparation method of a wound repair material with a cell growth regulation and control function, which comprises the following steps: S1, adding a high-molecular polymer and nano bioactive glass powder into a solvent to prepare an electrostatic spinning precursor solution; S2, sucking the electrostatic spinning precursorsolution prepared in the step S1 into an injector to carry out an electrostatic spinning process, and carrying out vacuum drying to obtain a polymer fiber film compounded with nano bioactive glass; S3, extracting a cell regulatory factor targeting gene segment, constructing a target gene plasmid, obtaining a plasmid DNA recombinant by using a gene recombination technology, and adding the plasmidDNA recombinant into the chitosan solution and the trehalose solution to obtain a mixed solution; and S4, coating the polymer fiber film obtained in the step S2 with the mixed solution obtained in thestep S3 to obtain the polymer fiber film. The wound repair material prepared by the invention has better histocompatibility, tensile strength, biological safety, antibacterial property and repair capability.

Description

technical field [0001] The invention relates to the field of skin wound repair materials, in particular to a preparation method of a wound repair material with cell growth regulation function. Background technique [0002] According to statistics, the annual demand for wound repair and regeneration caused by various acute and chronic skin injuries in my country is about 100 million person-times, and the average treatment cost for chronic wounds has reached 15,000 yuan in 2018. The acceleration of skin and mucous membrane damage caused by trauma, burns, scalds, chemical corrosion, bacterial and viral infections, and environmental pollution has increased significantly. The treatment of wound injuries (especially diabetic ulcers) has always been a difficult medical problem. problem. The use of biomedical materials to replace skin tissue has become a common treatment method at home and abroad. The global market size of biomaterials and their products exceeded 250 billion US dolla...

Claims

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Application Information

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IPC IPC(8): A61L15/26A61L15/28A61L15/18A61L15/44A61L15/46D01D5/00D04H1/728
CPCA61L15/18A61L15/26A61L15/28A61L15/44A61L15/46A61L2300/112A61L2300/232A61L2300/404A61L2300/412D01D5/003D01D5/0069D01D5/0092D04H1/728C08L5/08C08L5/04
Inventor 原波何显运丘永亮
Owner GUANGDONG POLYTECHNIC OF IND & COMMERCE
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