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Method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture

A technology for fermentation and cultivation of lignin, which is applied in the field of using mushroom fermentation and cultivation to convert lignin into phenolic acid, can solve the problems of polluting the environment, easily generating a large amount of waste liquid, waste residue and waste gas, and achieves small pollution opportunities, high output, low cost cost effect

Pending Publication Date: 2020-09-11
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the use of lignin to produce phenolic compounds mostly adopts chemical conversion methods, and catalysts are used to depolymerize lignin into small molecule phenolic compounds through liquefaction, pyrolysis, oxidation and reduction, etc., but this method is prone to produce a large amount of waste liquid , waste residue and exhaust gas, polluting the environment, and the biological conversion method is relatively more friendly to the environment

Method used

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  • Method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture
  • Method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture
  • Method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture

Examples

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preparation example Construction

[0032] The preparation method of improved potato dextrose broth medium:

[0033] Take 200g of potatoes, 20g of glucose, 0.5-2g of lignin, 20g of hemicellulose, and deionized water;

[0034] Cut the potatoes into small pieces, put them in deionized water, boil for a certain period of time (such as 20-30min), filter with multi-layer gauze, take the filtrate, add water to make it 1000ml, add glucose, lignin, and hemicellulose to dissolve completely, Adjust the pH to 6.0, and after aliquoting, sterilize under high pressure steam at 126° C. and 0.15 MPa for 30 minutes to obtain the modified potato dextrose broth medium.

[0035] Lentinus edodes mycelium fermentation and cultivation method: inoculate the lentinus edodes mycelium under sterile conditions, shake the flask for 10 days in a constant temperature shaking incubator at 28° C. and 160 rpm, and collect the lentinus edodes mycelium by centrifugation.

[0036] The collected mushroom mycelium is freeze-dried and then the phenol...

Embodiment 1

[0038] A method utilizing shiitake mushrooms to ferment and transform lignin into phenolic acids, the method comprising:

[0039] a) using improved potato dextrose broth medium, inoculating shiitake mushroom mycelia under aseptic conditions, and culturing in a shaking flask at 28° C. and 160 rpm in a constant temperature shaking incubator for 10 days;

[0040] Wherein, the concentration of lignin in the improved potato dextrose broth medium is 0.05% g / mL, and the concentration of hemicellulose is 2% g / mL;

[0041] b) collecting the mycelia and freeze-drying, and calculating the mycelium biomass;

[0042] Mycelium biomass was measured as follows: y=m 1 / v 1 , where y is mycelium biomass (g / L), m 1 is the dry weight of mycelium (g), v 1 is the volume of medium (L);

[0043]c) Dissolve the freeze-dried mycelia powder in acetonitrile and hydrochloric acid solution, ultrasonically extract for 30 minutes, bathe in 30°C water for 2 hours, and spin dry; redissolve in methanol to ...

Embodiment 2

[0046] This example provides a method for transforming lignin into phenolic acids by fermenting and cultivating shiitake mushrooms, which is basically the same as Example 1, except that in step a), the concentration of lignin in the improved potato dextrose broth medium 0.1% g / mL.

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Abstract

The invention belongs to the technical field of biological culture and particularly relates to method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture. The method comprises the steps of taking lignin as an exogenous additive, and preparing an improved potato dextrose broth medium containing a certain concentration of lignin; inoculating a lentinus edodes mycelium, culturing the lentinus edodes mycelium for a period of time and then collecting the lentinus edodes mycelium; and extracting and obtaining phenolic acid substances from the collected lentinus edodes mycelium. According to the method provided by the invention, growth of the lentinus edodes mycelium and biotransformation of phenolic compounds can be promoted through adding an appropriate concentration of lignin.

Description

technical field [0001] The invention belongs to the technical field of biological culture, and in particular relates to a method for transforming lignin into phenolic acid by fermenting and cultivating shiitake mushrooms. Background technique [0002] Lignin is the most abundant aromatic compound in nature, and it is a hyperbranched polymer composed of three phenylpropane monomers, coniferyl alcohol, sinapyl alcohol and coumaryl alcohol, linked by aryl ether and alkyl bonds. According to the structure of lignin, the derivatives obtained during the depolymerization process of lignin are generally phenolic acid compounds, including protocatechuic acid, vanillin, p-coumaric acid, ferulic acid, gallic acid, etc. Phenolic acid compounds are widely used in medicine, food, chemical industry and other fields. Biodegradation of lignin can reduce environmental pollution and realize the reuse of resources. At present, the biotechnology of using lignin-degrading fungi to produce phenol...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14C12P7/42C12P7/24C12R1/645
CPCC12N1/38C12N1/14C12P7/42C12P7/24
Inventor 张秀清殷丽君吴非霏葛鑫会
Owner CHINA AGRI UNIV
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