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Multifunctional stent coating based on recombinant human collagen and preparation method of multifunctional stent coating

A human collagen, multifunctional technology, applied in the field of multifunctional scaffold coating and its preparation based on recombinant human collagen, to achieve the effect of improving stability

Active Publication Date: 2020-08-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on the use of recombinant human collagen for the construction of multi-functional coated stent coatings

Method used

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  • Multifunctional stent coating based on recombinant human collagen and preparation method of multifunctional stent coating

Examples

Experimental program
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Embodiment 1

[0028] A method for preparing a multifunctional stent coating based on recombinant human collagen, comprising the following steps:

[0029] (1) Treat the pretreated cobalt-chromium alloy material in the MES buffer solution of EDC with a concentration of 0.5mg / ml for 30min, and then transfer to the NHS solution of 0.5mg / ml for activation for 30min;

[0030] (2) Place the product obtained in step (1) in a polyethyleneimine solution with a concentration of 5 mg / ml and a pH value of 8, and react at room temperature for 2 hours;

[0031] (3) Place the product obtained in step (2) in a 0.1 mg / ml chondroitin sulfate solution with a pH value of 3, and react at room temperature for 5 minutes;

[0032] (4) Put the product obtained in step (3) in a 0.1 mg / ml recombinant human collagen type III solution with a pH value of 3, and react at room temperature for 5 minutes, wherein the amino acid of the recombinant human type III collagen The sequence is GERGAPGFRGPAGPNGIPGEKGPAGERGAP;

[00...

Embodiment 2

[0035] A method for preparing a multifunctional stent coating based on recombinant human collagen, comprising the following steps:

[0036] (1) Treat the pretreated cobalt-chromium alloy material in the MES buffer solution of EDC with a concentration of 5mg / ml for 2h, then transfer to the NHS solution of 5mg / ml for activation for 2h;

[0037] (2) Place the product obtained in step (1) in a polylysine solution with a concentration of 10 mg / ml and a pH value of 8, and react at room temperature for 2 hours;

[0038] (3) Place the product obtained in step (2) in a 2 mg / ml chondroitin sulfate solution with a pH value of 4, and react at room temperature for 30 minutes;

[0039] (4) Place the product obtained in step (3) in a 2 mg / ml, pH value of 4 recombinant type III human collagen solution, and react at room temperature for 30 minutes, wherein the amino acid sequence of the recombinant human type III collagen for GERGAPGFRGPAGPNGIPGEKGPAGERGAP;

[0040] (5) At room temperature, ...

Embodiment 3

[0042] A method for preparing a multifunctional stent coating based on recombinant human collagen, comprising the following steps:

[0043] (1) the chitosan material after pretreatment is placed in the dopamine hydrochloride (DPA) solution of 2mg / ml, reacts 2h at room temperature;

[0044] (2) Place the product obtained in step (1) in a polyethylenediamine solution with a concentration of 10 mg / ml and a pH value of 7.4, and react at room temperature for 3 hours;

[0045] (3) The product obtained in step (2) was placed in a 2 mg / ml heparin solution with a pH value of 6, and reacted at room temperature for 30 minutes;

[0046] (4) Place the product obtained in step (3) in a 2 mg / ml, pH value of 6 recombinant type III human collagen solution, and react at room temperature for 30 minutes; wherein, the amino acid sequence of the recombinant human type III collagen for GERGAPGFRGPAGPNGIPGEKGPAGERGAP;

[0047] (5) At room temperature, using the product obtained in step (2) as a sub...

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Abstract

The invention provides a multifunctional stent coating based on recombinant human collagen and a preparation method of the multifunctional stent coating. The preparation method comprises the followingsteps: (1) activating a substrate material; (2) placing the activated substrate material into a solution containing an amino compound to react to obtain an aminated substrate material; (3) placing the aminated substrate material into a poly-negative electrolyte solution to react and then washing the aminated substrate material with de-ionized water; (4) placing the product obtained in step (3) into a poly-positive electrolyte solution to react and then washing the product with de-ionized water; and (5) taking the product obtained in step (2) as a substrate at room temperature and repeating the step (3) to the step (4) for 3-15 times to prepare the multifunctional stent coating. The multifunctional stent coating prepared by the invention can be used for effectively improving anticoagulation and rapid endothelialization properties of biological materials including vascular stent materials and the like, and the problems of late thrombus and restenosis of a current stent material are potentially reduced.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a multifunctional stent coating based on recombinant human collagen and a preparation method thereof. Background technique [0002] With the increasing pressure of people's life and irregular work, rest and diet, the death rate of cardiovascular disease in my country has exceeded 40%, and it has surpassed tumors to become the number one killer of disease-caused death, threatening the safety of human life. For a long time, cardiovascular materials have faced the major problems of anticoagulation and incomplete endothelialization. Excellent surface anticoagulant properties and accelerated in situ endothelialization are the keys to the success of blood-contact materials. Minimally invasive, efficient and safe stent implantation has become the main method for the treatment of cardiovascular diseases. At present, the drug-coated metal vascular stent used on a la...

Claims

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Application Information

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IPC IPC(8): A61L31/10A61L31/02A61L31/04A61L31/06A61L31/14A61L31/16
CPCA61L31/10A61L31/022A61L31/042A61L31/06A61L31/14A61L31/16A61L2400/18A61L2420/02A61L2300/42A61L2300/416A61L2420/08A61L2300/252C08L89/00C08L5/08C08L5/10C08L77/04C08L67/04B05D2202/00B05D1/18B05D3/104B05D3/107C08L89/06
Inventor 王云兵杨立张兴栋吴豪爽罗日方陆路杨霞
Owner SICHUAN UNIV
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