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Application of atractylodes in the preparation of inhibitors of Propionibacterium acnes

A technology of Propionibacterium acnes and atractylodes, which is applied in the field of medicine to achieve strong inhibitory activity and significant inhibitory effect

Active Publication Date: 2021-02-26
广东华夏友美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Studies in the prior art have proved that atractylodes has anti-inflammatory, anti-tumor, hypoglycemic, anti-viral and other activities, and about the inhibitory effect of atractylodes on Propionibacterium acnes and its role in inhibiting the excessive proliferation of Propionibacterium acnes in skin diseases Application not reported

Method used

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  • Application of atractylodes in the preparation of inhibitors of Propionibacterium acnes
  • Application of atractylodes in the preparation of inhibitors of Propionibacterium acnes
  • Application of atractylodes in the preparation of inhibitors of Propionibacterium acnes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 In vitro inhibitory effect of atractylodes on Propionibacterium acnes

[0040] 1. Materials and instruments

[0041] Test strain Propionibacterium acnes ATCC 11827 (Guangdong Institute of Microbiology Culture Collection Center), atractylodes (purchased from Chengdu Pusi Technology Co., Ltd., 98%); sterile PBS (pH7.4), ampicillin sodium powder, Metronidazole, BHI Broth Medium (Guangdong Huankaiwen Kesheng Microbial Technology Co., Ltd.), BHI Solid Medium (HB8478 Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.).

[0042] Ultra-clean bench (Suzhou Antai Air Technology Co., Ltd.), microscope (Chongqing Auto Optical Instrument Co., Ltd.), constant temperature incubator (DNP-9052 Ningbo Jiangnan Instrument Factory), electronic balance (BSA124S Sartorius Co., Ltd.), autoclave Pot, anaerobic gas generating bag (Mitsubishi), anaerobic bag (Mitsubishi), anaerobic indicator (Mitsubishi), sterilized filter paper, sterilized cotton balls, 96-well plate (Corning). Liqu...

Embodiment 2

[0055] Example 2: Atractylodes effect on acne rabbit ear model

[0056] 1. Materials and instruments

[0057] Fusidic acid cream (medical product registration certificate number: HC20090033); ether, water is ultrapure water, and other reagents are of analytical grade; coal tar (Jiangmen Hengjian Pharmaceutical Co., Ltd.); test strain Propionibacterium acnes ATCC 11827 (Guangdong Institute of Microbiology Culture Collection Center).

[0058] Healthy 10-12-week-old female New Zealand rabbits, 36, weighing 2Kg, were provided by Guangdong Experimental Animal Center, No. 44411600000809; license number: SYXK (Guangdong) 2012-0125.

[0059] The paraffin embedding machine EG-1160, the paraffin slicer RM2255, and the paraffin slice bleaching and drying instrument TEC-2500 are all manufactured by the German Lycra company. LKB 2188 ultramicrotome (NOVA), inverted optical microscope (Hitachi).

[0060] 2. Experimental steps

[0061] P. acnes is a Gram-positive anaerobic bacterium and ...

Embodiment 3

[0076] Embodiment 3: Chicken chorioallantoic membrane (CAM) test of atractylodes

[0077]The chorioallantoic membrane (CAM) test is one of the widely used models among the alternatives to eye irritation tests. Chicken chorioallantoic membrane is a kind of extracorporeal circulatory system of chicken embryo, which has a complete tissue rich in blood vessels. Using the similar characteristics of CAM and conjunctiva structure, by observing the changes of blood vessels (congestion, bleeding, coagulation) after CAM is exposed to chemical substances ), to detect the damage of chemical substances to CAM, and to evaluate the possible irritation of chemicals.

[0078] In order to further determine the irritation of atractylodes, the stimulation test of atractylodes with different concentrations on the allantoic membrane of chicken embryos was carried out to test the irritation of atractylodes, so as to provide sufficient evidence for the application of atractylodes in external skin pro...

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Abstract

The invention discloses the application of atractylodesin in the preparation of inhibitors of Propionibacterium acnes, the chemical formula of atractylodesin is C 13 h 10 O, the chemical name is 1,7-nonadiene-3,5-diyne furan; at the same time, atractylodes can be used to prepare the skin topical Propionibacterium acnes inhibitor, which has a good therapeutic effect on its related diseases, especially acne . The acne propionic acid inhibitor prepared by the atractylodes used in the present invention has the advantages of being safe, non-irritating, and has no side effects, has a certain preventive effect on diseases related to the excessive proliferation of Propionibacterium acnes, and can be widely used in the preparation of acne propionic acid for external use on the skin Bacillus inhibitors and products for the prevention and treatment of related diseases caused by the excessive proliferation of Propionibacterium acnes.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of atractylodes atractylodis in the preparation of medicaments for treating acne. Background technique [0002] Propionibacterium acnes (P.acnes) is a Gram-positive anaerobic short bacterium, an intracellular parasite, which belongs to the normal flora of the skin, and generally resides in the hair follicles or sebaceous glands of the skin. As adolescents mature, horn plugs appear at the hair follicle opening, and the secretion function of the sebaceous glands also increases significantly. Because sebum contains more fatty acids and other components, it is suitable for the growth and reproduction of P.acnes, and the excessive proliferation of Propionibacterium acnes produces an inflammatory response, thus One of the leading causes of acne. [0003] Propionibacterium acnes plays an extremely important role in the formation of acne inflammation. Acne patients wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/34A61P17/10A61P31/04A61K8/49A61Q17/00A61Q19/00
CPCA61K8/4973A61K31/34A61Q17/005A61Q19/00A61P17/10A61P31/04
Inventor 沈志滨胡锡昌张衍湖邓榕榕韩垂银吴焕玲
Owner 广东华夏友美生物科技有限公司
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