Application of atractylodin in preparation of propionibacterium acnes inhibitor
A technology of Propionibacterium acnes and atractylodes, which is applied in the field of medicine to achieve the effect of strong inhibitory activity and significant inhibitory effect
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Embodiment 1
[0039] Example 1 In vitro inhibitory effect of atractylodes on Propionibacterium acnes
[0040] 1. Materials and instruments
[0041] Test strain Propionibacterium acnes ATCC 11827 (Guangdong Institute of Microbiology Culture Collection Center), atractylodes (purchased from Chengdu Pusi Technology Co., Ltd., 98%); sterile PBS (pH7.4), ampicillin sodium powder, Metronidazole, BHI Broth Medium (Guangdong Huankaiwen Kesheng Microbial Technology Co., Ltd.), BHI Solid Medium (HB8478 Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.).
[0042] Ultra-clean bench (Suzhou Antai Air Technology Co., Ltd.), microscope (Chongqing Auto Optical Instrument Co., Ltd.), constant temperature incubator (DNP-9052 Ningbo Jiangnan Instrument Factory), electronic balance (BSA124S Sartorius Co., Ltd.), autoclave Pot, anaerobic gas generating bag (Mitsubishi), anaerobic bag (Mitsubishi), anaerobic indicator (Mitsubishi), sterilized filter paper, sterilized cotton balls, 96-well plate (Corning). Liqu...
Embodiment 2
[0055] Example 2: Atractylodes effect on acne rabbit ear model
[0056] 1. Materials and instruments
[0057] Fusidic acid cream (medical product registration certificate number: HC20090033); ether, water is ultrapure water, and other reagents are of analytical grade; coal tar (Jiangmen Hengjian Pharmaceutical Co., Ltd.); test strain Propionibacterium acnes ATCC 11827 (Guangdong Institute of Microbiology Culture Collection Center).
[0058] Healthy 10-12-week-old female New Zealand rabbits, 36, weighing 2Kg, were provided by Guangdong Experimental Animal Center, No. 44411600000809; license number: SYXK (Guangdong) 2012-0125.
[0059] The paraffin embedding machine EG-1160, the paraffin slicer RM2255, and the paraffin slice bleaching and drying instrument TEC-2500 are all manufactured by the German Lycra company. LKB 2188 ultramicrotome (NOVA), inverted optical microscope (Hitachi).
[0060] 2. Experimental steps
[0061] P. acnes is a Gram-positive anaerobic bacterium and ...
Embodiment 3
[0076] Embodiment 3: Chicken chorioallantoic membrane (CAM) test of atractylodes
[0077]The chorioallantoic membrane (CAM) test is one of the widely used models among the alternatives to eye irritation tests. Chicken chorioallantoic membrane is a kind of extracorporeal circulatory system of chicken embryo, which has a complete tissue rich in blood vessels. Using the similar characteristics of CAM and conjunctiva structure, by observing the changes of blood vessels (congestion, bleeding, coagulation) after CAM is exposed to chemical substances ), to detect the damage of chemical substances to CAM, and to evaluate the possible irritation of chemicals.
[0078] In order to further determine the irritation of atractylodes, the stimulation test of atractylodes with different concentrations on the allantoic membrane of chicken embryos was carried out to test the irritation of atractylodes, so as to provide sufficient evidence for the application of atractylodes in external skin pro...
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