Pseudomonas chlororaphis and culture method and applications thereof
A technology of Pseudomonas aeruginosa and an application method, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of reducing soil fertility, reducing the uniformity of seedling emergence, producing phytotoxicity, etc. The effect of root vitality, promoting plant growth and reducing side effects
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Embodiment 1
[0041] This embodiment provides a kind of Pseudomonas chloropinus screened from the plant rhizosphere growth-promoting bacterial flora in wheat roots and rhizosphere soil thereof, and the method for obtaining and cultivating Pseudomonas chloropinus comprises the following steps:
[0042] Acquisition of bacterial strains: Cut 1g of the root of wheat belt soil with excellent growth and health, grind it with a mortar, and dilute it to 10,000 times with sterile water. Spread the diluted mixture evenly on a 1 / 10TSA medium plate. Place it upside down in a constant temperature incubator at 28°C for 48 hours. After the strains grow out, use a sterile toothpick to pick bacterial colonies with different sizes, colors and shapes, and streak them on the TSA medium plate to separate and purify them. It is stored in a -70°C ultra-low temperature refrigerator; the isolated and purified strains are repeatedly screened through laboratory tests and field tests to obtain strains that can prevent ...
Embodiment 2
[0046] The present embodiment provides a kind of Pseudomonas chlororaphis bacterium liquid, and it is that the Pseudomonas chlororaphis Pc-HG28-5 that embodiment 1 provides and concentration are 0.1mol / l sterile magnesium sulfate solution preparations suspension, and the concentration of Pseudomonas aeruginosa in the Pseudomonas aeruginosa liquid is 2 × 10 8 CFU / ml.
Embodiment 3
[0048] This embodiment provides a kind of Pseudomonas chloroacinatus bacterial liquid, which is obtained by mixing a buffer solution containing magnesium sulfate with the Pseudomonas chloroacinatus, and the Pseudomonas chloroacinatus bacterial liquid contains The concentration of Pseudomonas was 1 x 10 8 CFU / ml; based on 100 parts by mass, the buffer solution includes: 0.02 part of sodium citrate, 0.15 part of ammonium sulfate, 0.5 part of dipotassium hydrogen phosphate, 0.1 part of potassium dihydrogen phosphate, 0.03 part of magnesium sulfate, 0.5 part of glycerin, The balance is sterile water.
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