a cufe 2 o 4 Preparation of nano-microsphere electrochemical sensor and detection method for lysozyme

A technology of nano-microspheres and lysozyme, applied in the field of electrochemical sensing, achieves the effects of good selective adsorption capacity, small particle size and good electrical conductivity

Active Publication Date: 2020-11-03
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as we know, CuFe 2 o 4 Hybrid material MIP sensors have not been reported yet

Method used

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  • a cufe <sub>2</sub> o <sub>4</sub> Preparation of nano-microsphere electrochemical sensor and detection method for lysozyme
  • a cufe <sub>2</sub> o <sub>4</sub> Preparation of nano-microsphere electrochemical sensor and detection method for lysozyme
  • a cufe <sub>2</sub> o <sub>4</sub> Preparation of nano-microsphere electrochemical sensor and detection method for lysozyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Carboxyl modified CuFe 2 O 4 Synthesis of nano-microspheres:

[0036] 1) Weigh 4.8 mmol ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 2.4 mmol copper chloride dihydrate (CuCl 2 ·2H 2 O) Dissolve 2 mol of trisodium citrate in 60 mL of ethylene glycol, and dissolve by ultrasound;

[0037] 2) Weigh 3.6g of sodium acetate (NaAc) and 1g of polyethylene glycol 20000 and slowly add the above solution, and stir vigorously for 24 hours at room temperature;

[0038] 3) Transfer the above reaction product to an 80mL PTFE-lined stainless steel autoclave, and heat it at 200°C for 10 hours. After the reaction, the autoclave is naturally cooled to room temperature;

[0039] 4) Collect the above reaction product by centrifugation, first wash it with ethanol / water (1:1; v:v) once, then wash it twice with deionized water, freeze-dry the product to obtain CuFe 2 O 4 Nanospheres

[0040] Carboxyl modified CuFe 2 O 4 Synthesis of imprinted polymer on the surface of nanospheres:

[0041] 5) Weigh 100 m...

Embodiment 2

[0052] Except for "Step 6), the molar ratio of acrylamide: N-isopropylacrylamide: methacrylic acid: N, N-methylenebisacrylamide is 2.5: 5.4: 2: 1", other steps and The operation is the same as that of Example 1.

Embodiment 3

[0054] Except for CuFe in "Steps 5) to 7) 2 O 4 Nanospheres: N, N-methylenebisacrylamide: ammonium persulfate: tetramethylethylenediamine with a molar ratio of 5.3: 4.5: 2.3: 1", other steps and operations are the same as those in Example 1. All the same.

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Abstract

The invention provides a preparation method of a molecularly imprinted polymer with carboxyl modified CuFe2O4 nano-microspheres, and belongs to the field of electrochemical sensing. According to the preparation method of the molecularly imprinted polymer with the carboxyl modified CuFe2O4 nano-microspheres, the carboxyl modified CuFe2O4 nano-microspheres with small particle size and uniform size are synthesized through a hydrothermal method. The carboxyl modified CuFe2O4 nano-microspheres are used as carriers, the molecularly imprinted polymer with the CuFe2O4 nano-microspheres is prepared through a surface imprinting technology, nanoparticles are then deposited on the surface of an electrode, a novel sensor with high selectivity and high response is prepared, operation is convenient, thecost is lowered, and the efficiency and sensitivity of a complex detection technology are improved. The sensor has high specific capacitance characteristic and good conductivity, under optimal conditions, the constructed multifunctional sensor has large detection range and low detection limit on target lysozyme. A further competitive selection experimental results show that the sensing material has high selectivity and sensitivity on measurement of the lysozyme, is successfully applied to detection of target proteins in complex biological samples, and has broad application prospects in the research fields such as disease diagnosis, food detection and biosensing.

Description

Technical field [0001] The invention belongs to the field of electrochemical sensing, and particularly relates to a carboxyl modified CuFe 2 O 4 Preparation method of imprinted polymer on the surface of nano-microsphere. Background technique [0002] In recent years, lyzase (lyz) has been considered as one of the potential biochemical markers for the diagnosis of leukemia and other diseases, which can mark changes or damages in human organs, tissues, cells, etc. In fact, lysozyme is widely present in plants, animals, microorganisms and human tissues. Its relative molecular weight is low (14.4 kDa), strong heat resistance, strong cold tolerance, and stable activity. It is often selected as a target protein for biochemical research. . At present, the conventional detection techniques of lysozyme mainly focus on enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC), mass spectrometry (MS), fluorescence detection (FD) and capillary gel electrophore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 罗爱芹梁阿新汤波
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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