Saccharomyce sp. ZB412 and application thereof
A ZB412, yeast technology, applied in the direction of fermentation, fungi, microorganisms, etc., can solve the problems of increasing production energy consumption and production cost, unable to meet the demand for vinegar flavor, and the generation of unfavorable aroma substances, so as to improve the utilization rate of raw materials , good operability and economic benefits, good taste and sweetness
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Embodiment 1
[0041] Embodiment 1 strain mutagenesis
[0042] Saccharomyces ZB118 is used as the starting strain, and the acquisition of yeast ZB118 refers to the method described in the Chinese patent "A Yeast and Its Application" with publication number CN108315271A. The biological deposit information of yeast ZB118 is as follows: it was deposited in the Guangdong Provincial Microbial Culture Collection Center on November 17, 2017, named as yeast (saccharomyce sp.) ZB118, the deposit number is GDMCC NO:60280, and the deposit address is Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou. The mature slant of the strain ZB118 was made into a bacterial suspension, and the yeast suspension was subjected to ARTP mutagenesis. Dilute the mutagenized yeast suspension to 10 with sterile saline -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration, make bacterial cell dilution, count the bacterial cell dilution of different concentrations under the microscope, choose 10 -3 Concentra...
Embodiment 2
[0043] The screening of embodiment 2 mutagenic strains
[0044] (1) Primary screening of mutagenized strains
[0045] Pipette 0.1 mL of the bacterial cell dilution obtained in Example 1, and evenly spread it on the YPD solid medium supplemented with 0.4% tributyrin, and incubate at 28-30°C in the dark for 48 hours, and observe the growth of colonies during the cultivation process The situation and the speed of the formation of the transparent circle, and record the diameter of the colony growth and the diameter of the transparent circle. The ratio of the diameter of the transparent circle to the diameter of the colony is used to characterize the ester-producing ability of the yeast strain. Therefore, screening is carried out according to the ratio of the diameter of the transparent circle to the diameter of the colony. Compared with the starting strain, the colony has good colony growth and better ester production performance. After screening, six strains with better ester-p...
Embodiment 3
[0058] Example 3 Saccharomyces ZB412 strain genetic stability detection
[0059] The yeast strain ZB412 screened out in Example 2 was inoculated on the YPD slant medium for 10 generations, and the growth of each generation strain was observed, and the 1st generation, the 5th generation and the 10th generation slant strain were inoculated according to Example 2. According to the method in the middle method, the strains were fermented and cultivated. After the fermentation, the contents of 4-ethylguaiacol and ethyl acetate in the fermentation liquid were determined by high-performance liquid chromatography, and the genetic stability was judged. If the content error of 4-ethylguaiacol and ethyl acetate in the obtained fermentation broth is within 10%, it indicates that the genetic stability of the strain is good, and the specific data are shown in Table 3.
[0060] Table 3 Yeast strain subculture stability test results
[0061]
[0062] As shown in the results in Table 3, fro...
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