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Anterior chamber perfusion solution for intraocular surgery and application thereof

A technology for perfusion fluid and surgery, applied in the field of medicine, can solve problems such as low solubility and unstable properties of glutamine, and achieve the effects of protecting cells, improving cell anti-oxidation, and good surgical results

Active Publication Date: 2019-05-21
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a kind of anterior chamber perfusion fluid for intraocular surgery and its application, under the effect of balancing intraocular pressure, it also contains a certain amount of L-alanyl- L-glutamine solves the shortcomings of glutamine's unstable properties and low solubility; the anterior chamber perfusion fluid for intraocular surgery not only has a protective effect on ischemia and hypoxia, but also can provide nutrients. It is used in intraoperative perfusion, When the perfusion solution acts on corneal endothelial cells, it can reduce the number of corneal endothelial cell apoptosis, reduce postoperative corneal swelling, and achieve better surgical results

Method used

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  • Anterior chamber perfusion solution for intraocular surgery and application thereof
  • Anterior chamber perfusion solution for intraocular surgery and application thereof
  • Anterior chamber perfusion solution for intraocular surgery and application thereof

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Experimental program
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Effect test

Embodiment 1

[0017] The preparation of embodiment 1 intraocular surgery anterior chamber perfusion fluid:

[0018] The anterior chamber perfusion solution for intraocular surgery of this embodiment contains the following components per 1 mL:

[0019] Sodium chloride 5.95~6.00mg

[0020] Potassium chloride 0.29~0.30mg

[0021] Calcium chloride 0.19~0.20mg

[0022] Sodium Lactate 3.05~3.15mg

[0023] L-alanyl-L-glutamine 0.217~1.086mg.

Embodiment 2

[0024] Example 2 Perfusate treatment cornea experiment and anterior chamber perfusion experiment

[0025] 1. Subjects

[0026] Thirty healthy and clean ICR mice aged 6-8 weeks, weighing 20-25 g, male or female.

[0027] 2. Experimental reagents

[0028] L-alanyl-L-glutamine, TUNEL apoptosis kit, F-actin.

[0029] 3. Experimental equipment

[0030] Operating microscope, upright multiphoton laser confocal system Olympus FV1000MPE-B, scanning electron microscope Jeo1JSM-6390LV, Heidelberg confocal laser corneal microscope.

[0031] 4. Experimental method

[0032] 1. Isolation of the Cornea

[0033] The mice were sacrificed by cervical dislocation, and the eyes were enucleated. After rinsing with normal saline, the mouse cornea was cut along the limbus to remove the iris and ciliary body. The operation of separating the cornea was carried out under an operating microscope to prevent mechanical damage to the cornea.

[0034] 2. Treat the cornea

[0035] 2.1 In vitro experi...

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Abstract

The invention discloses an anterior chamber perfusion solution for intraocular surgery and application thereof. Under the effect of balanced intraocular pressure, the anterior chamber perfusion solution further contains a certain amount of L-alanyl-L-glutamine. Long-time liquid perfusion is required for intraocular surgery such as intraocular lens implantation and vitrectomies, conventional ophthalmic perfusion fluid is a balanced salt solution and lacks in oxygen and nutrients, and the influence of the balanced salt solution on corneal endothelial cells cannot be ignored. The anterior chamberperfusion solution for the intraocular surgery is applied to perfusion during surgery, can not only have a protection effect on ischemia-hypoxia but also provide nutrients, and can reduce the apoptosis quantity of the corneal endothelial cell when acting on the corneal endothelial cells and alleviate postoperation corneal swelling to achieve a better surgery effect.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an anterior chamber perfusate for intraocular surgery and its application. Background technique [0002] With the development of microscope technology, intraocular surgery is becoming more and more mature, and the number is increasing year by year, saving many blind patients. In intraocular surgery, intraocular perfusion is essential to balance intraocular pressure. Extracapsular cataract extraction, intraocular lens implantation, and vitrectomy all require a long period of fluid perfusion. Conventional intraocular perfusion solution is ophthalmic balanced salt solution, which lacks oxygen and does not contain nutrients. During a long operation, it will be in contact with corneal endothelial cells for a long time, which will easily cause the cells to lose their activity or even undergo apoptosis. The visual acuity recovery of postoperative patients has a lot to do with the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K47/02A61K47/12A61K31/198A61P41/00
CPCA61P41/00A61K31/198A61B2217/007A61F9/007A61K9/0048A61K9/08A61K47/02A61K47/12
Inventor 李程金梦怡洪滢刘祖国
Owner XIAMEN UNIV
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