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Pyricularia grisea spore production culture medium and preparation method thereof

A culture medium preparation and culture medium technology, which is applied in the field of spore production medium and its preparation of blast fungus, can solve the problems of spore production of blast fungus, achieve stable spore production, simple steps, and improve work efficiency

Inactive Publication Date: 2019-04-16
GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of spore-producing culture medium of blast fungus and its preparation method, which can solve the problem of sporulation of blast fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 Raw tartary buckwheat culture medium, wheat bran culture medium and sorghum grain culture medium compare

[0013] Raw tartary buckwheat medium: raw tartary buckwheat 50g, agar 15g, water 1000~2000mL

[0014] Wheat bran medium: 50g wheat bran, 15g agar, 1000-2000mL water

[0015] Production method of tartary buckwheat / wheat bran medium: put 50g of raw tartary buckwheat / wheat bran into 1000mL of water and boil for 30 minutes, filter the solution with double gauze, put 15g of agar into the filtrate, and heat it in a microwave until the agar melts Then add water to make up to 1000mL. Pour it into 500mL Erlenmeyer flasks while hot, 200mL per bottle, put the Erlenmeyer flasks into a high-pressure steam sterilizer and sterilize at 121°C for 20 minutes for later use. Before inoculation, heat and melt the solid culture medium in the Erlenmeyer flask with a microwave oven to become liquid, and pour 9mm culture medium about 2-3mm high (about 20mL per dish) before co...

Embodiment 3

[0037] Embodiment 3 tartary buckwheat culture medium and the comparison of sporulation yield of improved oatmeal supernatant medium

[0038] Tartary buckwheat culture medium is made: with embodiment 1

[0039] Production of oatmeal serum medium: first add 50 grams of commercially available raw oatmeal to 1000 mL of water and boil for 30 minutes, then filter through double-layer gauze, add 20 grams of sucrose and 15 grams of agar, heat and melt, and make up water to 1000 mL. Pour it into a 500mL triangular flask while hot, about 200ml per bottle; sterilize it in a high-pressure steam sterilizer at 121°C for 20 minutes, and set aside.

[0040] Sporulation method of oatmeal serum medium: use a 5mm puncher to punch out the fungus flakes that have been multiplied on the PDA medium in advance to cover the entire dish. Put one piece per dish into the middle of the petri dish, cover the dish and place it upside down in a biochemical incubator at 26°C for static cultivation. On the 14t...

Embodiment 4

[0046] The spore production contrast of embodiment 4 tartary buckwheat culture medium and common cornmeal culture medium

[0047] Tartary buckwheat culture medium is made: with embodiment 1.

[0048] Production of cornmeal medium: First, add 20 grams of commercially available cornflour to 1000mL of water and boil for 20 minutes. After filtering through a single layer of gauze, put 12 grams of agar into it. Heat and melt to add water to 1000mL. Pour it into a 500mL triangular flask while hot, about 200ml per bottle; sterilize it in a high-pressure steam sterilizer at 121°C for 20 minutes, and set aside.

[0049] Sporulation method on corn flour medium: use a 5mm hole puncher to punch out the mycelium of the blast blast mycelia that has been propagated on the PDA medium in advance to make the whole dish full. Put one piece per dish into the middle of the petri dish, cover the dish and place it upside down in a biochemical incubator at 26°C for static cultivation. On the 14th d...

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Abstract

The invention discloses a pyricularia grisea spore production culture medium and a preparation method thereof. The culture medium comprises raw tartary buckwheat / wheat bran, agar and water. The preparation method of the culture medium comprises the following steps that the raw tartary buckwheat / wheat bran is mixed with water to be boiled, filtering is carried out, the agar is added into a filter liquor, after heating and melting are carried out, water is added until constant volume, and sterilization is carried out for standby application at high temperature and high pressure; before inoculation, the culture medium is heated and melted, and then cooled, and the culture medium is poured into a culture dish before solidification. The pyricularia grisea spore production culture medium has theadvantages that the formula components are easy to obtain, the formula is simple and reasonable, the preparation method of the culture medium is simple and convenient and easy to operate, the cultureprocess is not easy to contaminate, and the time and labor are saved.

Description

technical field [0001] The invention relates to the technical field of phytopathogenic fungi, in particular to a spore-producing medium for Pyricularia oryzae and a preparation method thereof. Background technique [0002] Grain blast is an important disease that seriously endangers millet production. The identification of millet resistance to valley blast and the screening of resistant materials are the prerequisites for cultivating improved millet varieties and ensuring safe millet production. However, the main factor that restricts the smooth progress of millet resistance to valley blast is the preparation of the inoculum solution containing a large number of conidia of the plague fungus. Whether a large amount of spores can be obtained at any time to prepare the bacterial solution for mass identification in the field is a major factor that restricts resistance identification. The bottleneck of work development is also an urgent problem for millet researchers to solve. ...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N1/14C12N3/00
Inventor 马继芳王永芳白辉李志勇董志平全建章郑直刘磊李建华
Owner GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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