Catechin biological conversion nutrient solution and method for promoting catechin biological conversion
A technology for biotransformation and catechin, applied in the field of bioengineering, can solve the problems of complex components of biotransformation nutrient solution, and achieve the effects of mild conditions, high safety and simple components for whole-cell biotransformation
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Embodiment 1
[0073] Prepare the biotransformation nutrient solution according to the following formula: 2% tea polyphenols, 0.5% yeast extract powder, 0.5% sodium nitrate, the balance is water, and sterilize at 121°C for 20 minutes.
[0074] Inoculate the Aspergillus niger RAF106 strain in PDA medium, and culture it statically at 28°C for 5 days;
[0075] Wash the plate covered with black Aspergillus niger spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus niger spores.
[0076] Adjust the spore suspension to 1 × 10 with sterile water 5 1 / mL, then add 1 mL of spore suspension into the biotransformation nutrient solution, and incubate at 160 r / min at 28°C for 50 h.
[0077] Take an appropriate amount of the transformation product every 10 hours, centrifuge at 8000rpm / min for 5min, take 1mL supernatant and dilute 100 times with ultrapure water, and filter with a 0.22µm filter membrane.
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Embodiment 2
[0080] Prepare the biotransformation nutrient solution according to the following formula: 3% tea polyphenols, 0.8% tryptone, 0.8% ammonium sulfate, the balance is water, and sterilize at 121°C for 20 minutes.
[0081] Aspergillus brasiliensis ATCC16404 was inoculated in PDA medium and cultured statically at 28°C for 3 days.
[0082] Wash the plate covered with black Aspergillus brasiliensis spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus brasiliensis spores.
[0083] Adjust the spore suspension to 1 × 10 with sterile water 6 1 / mL, then take 1 mL of the spore suspension and add it to the biotransformation nutrient solution, and incubate at 160 r / min at 32 °C for 80 h.
[0084] Take an appropriate amount of the transformation product every 10 hours, centrifuge at 10,000 rpm / min for 3 minutes, take 1 mL of the supernatant and dilute 100 times with ultrapure water, and filte...
Embodiment 3
[0087] Prepare the biotransformation nutrient solution according to the following formula: 3% EGCG, 1.0% sodium nitrate, 1.0% tryptone, 1.0% tryptophan, the balance is water, and sterilize at 121°C for 20 minutes.
[0088] Inoculate the Aspergillus niger RAF106 strain in PDA medium, and culture it statically at 35°C for 4 days.
[0089] Wash the plate covered with black Aspergillus niger spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus niger spores.
[0090] Adjust the spore suspension to 1 × 10 with sterile water 7 1 / mL, then take 1 mL of the spore suspension and add it to the biotransformation nutrient solution, and incubate at 160 r / min at 32 °C for 80 h.
[0091] Select an appropriate time point to take out an appropriate amount of the transformation product, centrifuge at 12000rpm / min for 2min, take 1mL of the supernatant and dilute it 100 times with ultrapure water, ...
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