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Catechin biological conversion nutrient solution and method for promoting catechin biological conversion

A technology for biotransformation and catechin, applied in the field of bioengineering, can solve the problems of complex components of biotransformation nutrient solution, and achieve the effects of mild conditions, high safety and simple components for whole-cell biotransformation

Inactive Publication Date: 2019-01-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a kind of catechin biotransformation nutrient solution in order to overcome the defect that the composition of the biotransformation nutrient solution described in the above prior art is complex and only converts EGCG into EGC and GA, and the composition of the biotransformation nutrient solution provided is simple and convenient. Separation and purification after cultivation can also promote the biotransformation of various catechins by Aspergillus

Method used

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  • Catechin biological conversion nutrient solution and method for promoting catechin biological conversion
  • Catechin biological conversion nutrient solution and method for promoting catechin biological conversion
  • Catechin biological conversion nutrient solution and method for promoting catechin biological conversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Prepare the biotransformation nutrient solution according to the following formula: 2% tea polyphenols, 0.5% yeast extract powder, 0.5% sodium nitrate, the balance is water, and sterilize at 121°C for 20 minutes.

[0074] Inoculate the Aspergillus niger RAF106 strain in PDA medium, and culture it statically at 28°C for 5 days;

[0075] Wash the plate covered with black Aspergillus niger spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus niger spores.

[0076] Adjust the spore suspension to 1 × 10 with sterile water 5 1 / mL, then add 1 mL of spore suspension into the biotransformation nutrient solution, and incubate at 160 r / min at 28°C for 50 h.

[0077] Take an appropriate amount of the transformation product every 10 hours, centrifuge at 8000rpm / min for 5min, take 1mL supernatant and dilute 100 times with ultrapure water, and filter with a 0.22µm filter membrane.

...

Embodiment 2

[0080] Prepare the biotransformation nutrient solution according to the following formula: 3% tea polyphenols, 0.8% tryptone, 0.8% ammonium sulfate, the balance is water, and sterilize at 121°C for 20 minutes.

[0081] Aspergillus brasiliensis ATCC16404 was inoculated in PDA medium and cultured statically at 28°C for 3 days.

[0082] Wash the plate covered with black Aspergillus brasiliensis spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus brasiliensis spores.

[0083] Adjust the spore suspension to 1 × 10 with sterile water 6 1 / mL, then take 1 mL of the spore suspension and add it to the biotransformation nutrient solution, and incubate at 160 r / min at 32 °C for 80 h.

[0084] Take an appropriate amount of the transformation product every 10 hours, centrifuge at 10,000 rpm / min for 3 minutes, take 1 mL of the supernatant and dilute 100 times with ultrapure water, and filte...

Embodiment 3

[0087] Prepare the biotransformation nutrient solution according to the following formula: 3% EGCG, 1.0% sodium nitrate, 1.0% tryptone, 1.0% tryptophan, the balance is water, and sterilize at 121°C for 20 minutes.

[0088] Inoculate the Aspergillus niger RAF106 strain in PDA medium, and culture it statically at 35°C for 4 days.

[0089] Wash the plate covered with black Aspergillus niger spores with sterile water containing 0.05% Tween-80, and gently scrape the surface of the medium with a pipette tip to obtain a suspension of Aspergillus niger spores.

[0090] Adjust the spore suspension to 1 × 10 with sterile water 7 1 / mL, then take 1 mL of the spore suspension and add it to the biotransformation nutrient solution, and incubate at 160 r / min at 32 °C for 80 h.

[0091] Select an appropriate time point to take out an appropriate amount of the transformation product, centrifuge at 12000rpm / min for 2min, take 1mL of the supernatant and dilute it 100 times with ultrapure water, ...

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Abstract

The invention discloses a catechin biological conversion nutrient solution and a method for promoting catechin biological conversion. The catechin biological conversion nutrient solution is prepared from raw materials containing catechin, a nitrogen source compound and water; the raw materials containing catechin are tea polyphenol or catechin; the mass percentage of the nitrogen source compound is 0.01-5%. The catechin biological conversion nutrient solution is simple in ingredient, contains no other complex inorganic salt, and causes no interference with catechin EGCG and the like; the catechin biological conversion nutrient solution is simple, convenient to use, rapid, environmentally friendly, low in cost and capable of being widely applied to the field of food and medicine; the addednitrogen source compound can improve the tannase activity, catechin EGCG is rapidly converted, and biological conversion of other catechin ECG, GCG, EGC, EC, GA, GC, CG, C and the like through aspergillus can be promoted.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a catechin biotransformation nutrient solution and a method for promoting catechin biotransformation. Background technique [0002] Catechin is a class of functional active ingredients rich in tea polyphenols, accounting for 65% to 80% of the total tea polyphenols, mainly including ester-type catechins and non-ester-type catechins, ester-type catechins Known as: Epigallocatechin gallate (Epigallocatechin gallate, EGCG), gallocatechin gallate (Gallocatechin gallate, GCG), epicatechin gallate (Epicatechin gallate, ECG), catechin gallate Ester (Catechingallate, CG); Non-ester type catechins: Epicatechin (EC), Epigallocatechin (Epigallocatechin, EGC), Gallocatechin (Gallocatechin, GC), Gallic acid (Gallocateacid) , GA). [0003] Catechin, especially EGCG, has a series of pharmacological functions such as scavenging free radicals, anti-aging, anti-radiation, anti-cancer and a...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/685C12R1/66
CPCC12P1/02C12N1/145C12R2001/66C12R2001/685
Inventor 王洁方祥杜敏如郑勇廖振林
Owner SOUTH CHINA AGRI UNIV
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