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Use of cardiospermum halicacabum extract in the manufacture of a composition for upregulating sod2 expression, promoting skin repair and preventing skin damage by UV light

A technology of gene expression and skin repair, applied in the direction of drug combinations, skin care preparations, skin diseases, etc., can solve the problems of inability to block UVA penetration, inability to completely eliminate ultraviolet damage, etc., to prevent or treat ultraviolet light damage, promote The effect of skin repair

Pending Publication Date: 2018-12-04
百岳特美肤生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although UVB can cause skin damage and aging, excessive exposure to the sun may lead to acute sunburn and chronic skin cancer, but UVA has strong penetrating power, and even ordinary transparent glass cannot block the penetration of UVA. The most susceptible to exposure to ultraviolet light
[0004] Therefore, we cannot completely eliminate the damage of ultraviolet rays in our daily life. Even if we are indoors, such as driving a car or doing activities near the indoor window, we will be exposed to UVA exposure

Method used

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  • Use of cardiospermum halicacabum extract in the manufacture of a composition for upregulating sod2 expression, promoting skin repair and preventing skin damage by UV light
  • Use of cardiospermum halicacabum extract in the manufacture of a composition for upregulating sod2 expression, promoting skin repair and preventing skin damage by UV light
  • Use of cardiospermum halicacabum extract in the manufacture of a composition for upregulating sod2 expression, promoting skin repair and preventing skin damage by UV light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 skin fibroblasts anti-UVA ability test:

[0027] Experimental equipment and materials:

[0028] 1. Cell line: human dermal fibroblasts (CCD-966sk; BCRC No.60153);

[0029] 2. Add Earle's BSS, 0.1mM non-essential amino acids, 1.5g / L sodium bicarbonate, 1mM sodium pyruvate to the minimum essential medium (Eagle); 10% fetal bovine serum (Gibco);

[0030] 3.4 mg / ml 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT; AMERSCO / 0793-5G) dissolved in phosphate buffered saline (PBS) ;

[0031] 4. Dimethylsulfoxide (DMSO; ECHO / DA1101-000000-72EC);

[0032] 5. Enzyme immune analyzer (ELISA reader; BioTek);

[0033] 6. Ultraviolet radiation chamber (Ultraviolet radiation chamber; Vilber);

[0034] Experimental steps:

[0035] 1. In a 96-well culture plate, implant 5×10 3 cells;

[0036] 2. Incubate at 37°C for 24 hours;

[0037] 3. Remove the culture medium without disturbing the attached cells;

[0038] 4. Treat with the reagent (n=4) and then in...

Embodiment 2

[0050] Example 2 Detection of Antioxidant Gene Ability of Skin Fibroblasts:

[0051] Experimental instruments and materials:

[0052] 1. Cells: human fibroblasts (CCD-996SK; ATCC);

[0053] 2. Culture medium: minimum essential medium (Eagle) with Earle's balanced salt solution (Earle's BSS), 0.1mM non-essential amino acids, 1.5g / L sodium bicarbonate, 1mM sodium pyruvate, accounting for 90%; fetal bovine serum 10% (Gibco);

[0054] 3. RNA extraction kit (Geneaid);

[0055] 4. III reverse transcriptase (Invitrogen);

[0056] 5. Primer sets of antioxidant-related genes (UNG, OGG1, APE1, XRCC1, XRCC5, MSH2, MLH1, MSH6, ERCC1, XPA, SOD1, SOD2, GPx, and CAT), including internal controls;

[0057] 6. KAPA CYBR FAST qPCR Set (2x) (KAPA Biosystems);

[0058] 7. Real-time polymerase chain reaction instrument (Step One Plus; ABI);

[0059] Experimental steps:

[0060] Cell preparation and stimulation

[0061] 1. In a 6-well plate, add 2ml of culture medium to each well and impla...

Embodiment 3

[0077] Example 3 Skin fibroblast damage repair fluorescence test:

[0078] 1. Cell line: human dermal fibroblasts (CCD-966sk; BCRC No.60153);

[0079] 2. Culture medium:

[0080] Minimal essential medium (Eagle) added Earle's BSS, 0.1mM non-essential amino acids (90%),

[0081] 1.5g / L sodium bicarbonate, 1mM sodium pyruvate, fetal bovine serum 10% (Gibco);

[0082] 3. Phosphate buffer solution (Gibco);

[0083] 4. Propidium iodide staining solution (BD Pharmingen; Cat.51-66211E);

[0084] 5. Hoechst 33342 (Thermo; Cat. 62249);

[0085] 6. Annexin V, Alexa Fluor 488 (Invitrogen; Cat.A13201);

[0086] 7. Annexin V binding buffer solution (eBioscience; Cat.00-0055-43);

[0087] 8. Ultraviolet irradiation room (Vilber);

[0088] 9. Microscope (ZEISS);

[0089] Experimental steps:

[0090] 1. Implant 2×10 in a 24-well dish 4 cells / well, and culture overnight at 37°C;

[0091] 2. Before UVA irradiation, add 0.5mg / ml kudzu fruit extract to the culture medium for pretreatme...

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Abstract

The invention relates to use of a cardiospermum halicacabum extract, and particularly to use of the cardiospermum halicacabum extract in the manufacture of a composition for upregulating SOD2 expression, promoting skin repair and preventing skin damage by UV llight. The cardiospermum halicacabum extract has a function of preventing or treating skin injury by the UV light.

Description

technical field [0001] The present invention relates to the use of kudzu extract, especially the use of kudzu extract to prepare a composition for improving the expression of SOD2 gene, promoting skin repair and preventing skin from being damaged by ultraviolet light. Background technique [0002] Ultraviolet (UV for short) is an electromagnetic wave that exists in sunlight, and its wavelength is from 10nm (30PHz) to 400nm (750THz). Ultraviolet light is a kind of harmful light. It is absorbed through the skin and causes damage to DNA. When DNA is damaged, cells will die or develop into uncontrollable cancer cells. This is the initial stage of tumor formation. UV rays have been linked to the development of many diseases; eg wrinkles, sunburn, cataracts, skin cancer, visual damage and damage to the immune system. [0003] After ultraviolet rays pass through the atmosphere, the remaining main ultraviolet rays can be subdivided into UVA (wavelength 320 to 400nm) and UVB (wavele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61K8/9789A61P17/16A61Q19/00A61Q17/04A61K131/00
CPCA61K36/77A61Q17/04A61Q19/004A61K8/9789A61P17/16
Inventor 林咏翔姚采涵
Owner 百岳特美肤生物技术(上海)有限公司
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