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Virulent Strain of Mycoplasma Hyorhina and Its Application

A technology of Mycoplasma hyorrhea and virulent strains, applied in the field of microorganisms, can solve the problems of incompatibility with onset characteristics, unrepresentativeness, late onset time, etc., and achieves good immune stimulation ability and anti-virus protection efficacy, early onset time, and toxic effects. strong effect

Active Publication Date: 2021-01-29
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the published isolates of Mycoplasma hyorhini either mainly have lung lesions, do not conform to the typical clinical characteristics of pig farms, and are not representative; Virus, the onset is relatively mild, the onset time is late, or it needs to use colostrum-free pigs to develop the disease

Method used

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  • Virulent Strain of Mycoplasma Hyorhina and Its Application
  • Virulent Strain of Mycoplasma Hyorhina and Its Application
  • Virulent Strain of Mycoplasma Hyorhina and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Isolation, cultivation and identification of Mycoplasma hyorhinois HEF16 strain

[0024] A pig group in a pig farm in Hefei, Anhui Province found joint swelling, lameness, depression, loss of appetite and other suspected symptoms of Mycoplasma hyorhina infection. The joint cavity fluid was collected, inoculated with KM2 medium, and a strain of Mycoplasma was isolated from it, which was sequenced by 16S rDNA PCR. The sequence is shown as SEQ ID NO: 1. After homology comparison, it was found that the homology with the Mycoplasma hyorhina sequence reached 99%, and it was identified as Mycoplasma hyorhina.

[0025] The mycoplasma isolated above was inoculated into healthy negative pigs through intranasal, intraperitoneal, or tracheal routes. Typical clinical symptoms appeared within one week after the challenge, and pathological changes related to multiple serositis and arthritis could be observed at autopsy, and they were isolated again. To Mycoplasma hyorhin...

Embodiment 2

[0034]Embodiment 2: The challenge pathogenesis test 1 of Mycoplasma hyorhinois HEF16 strain

[0035] Animals: three-way cross-breeding pigs with aseptic delivery and no colostrum intake, bred to 3 months old, all healthy after testing, used as challenge animals. There were 5 rats in the challenge group and 5 rats in the control group.

[0036] Attacking materials: Mycoplasma hyorhina HEF16 strain, seeded with KM2 medium at 37°C for 1-3 days to recover, and used after 2-3 generations.

[0037] Challenge: Take the fresh culture of Mycoplasma hyorhina HEF16 and adjust the titer to 10 7 CCU / mL, inoculate 5ml each via nasal drops and intrapulmonary injection, a total of 10 8 CCU per head.

[0038] Criteria for judging the onset: observe the clinical symptoms after inoculation, and observe the pathological changes after 21-28 days of necropsy. Any one of the following two items can be judged as the onset: (1) Mycoplasma hyorhinophysis infection causes death. (2) With obvious cli...

Embodiment 3

[0041] Embodiment 3: The challenge pathogenesis test 2 of Mycoplasma hyorhinois HEF16 strain

[0042] Animals: Binary crossbreed pigs with sterile inoculation and no colostrum feeding were bred to 2 months old, and all were healthy after testing, and were used as challenge animals. There were 5 rats in the challenge group and 5 rats in the control group.

[0043] Challenge: take the fresh culture of Mycoplasma hyorhini HEF16 strain (the culture method is the same as in Example 2), and adjust the titer to 10 7 CCU / mL, inoculate 5ml each via the intratracheal and intraperitoneal routes, a total of 10 8 CCU per head.

[0044] Incidence criteria: Same as above.

[0045] Clinical and autopsy observation results: 3 days after inoculation, the body temperature began to rise, and on the 4th day, poor appetite, joint swelling, lameness, joint palpation pain, rough coat, and depression began to appear, and the clinical symptoms lasted for more than 3 days. In the second week after t...

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Abstract

The invention provides a virulent strain of mycoplasma hyorhina and application thereof, and relates to the technical field of microbes. Mycoplasma hyorhina virulent strain of the present invention is mycoplasma hyorhina ( Mycoplasma hyorhinis ) HEF16 strain, the preservation number is CCTCC NO: M2017462. The present invention also provides the application of the Mycoplasma hyorhina in establishing a Mycoplasma hyorhina challenge pathogenesis model, using the pure culture of the virulent strain to challenge the virus can induce multiple Mycoplasma hyorhina including multiple serositis and arthritis Infection with typical symptoms, strong virulence, rapid onset, typical clinical manifestations can appear within 7 days, severe onset, and can occur on pigs with different backgrounds, can be used to establish a typical disease model of Mycoplasma hyorhinosum, which is representative. The invention also provides the application of the mycoplasma hyorhinois in preparing an inactivated vaccine, the strain has good immunogenicity, and the prepared inactivated vaccine has good challenge protection effect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a virulent strain of mycoplasma hyorhina and application thereof. Background technique [0002] Mycoplasma hyorhinosum ( Mycoplasma hyorhinis ), belonging to the Mycoplasma family (Mycoplasmataceae), a member of the genus Mycoplasma (Mycoplasma), was first isolated from the nasal cavity of pigs with infectious atrophic rhinitis by Carler and Mckay in 1953, so it was named Mycoplasma hyorhinosus. Mycoplasma hyorhinosum is a common pathogen in clinical pig farms. It is usually transmitted from sows or older pigs to piglets. It can generally be transmitted by upper respiratory tract infection through droplets or direct contact. Once pigs are infected, the mycoplasma spreads rapidly in the upper respiratory tract and can be isolated from the lungs and nasopharyngeal tubes of infected pigs, and then can migrate to the whole body through the respiratory tract. Mycoplasma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01K67/027A61K39/02A61P31/04C12R1/35
CPCA01K67/027A01K2227/108A01K2267/0337A61K39/0241A61K2039/521C12N1/205C12R2001/35
Inventor 熊祺琰王佳邵国青冯志新华利忠韦艳娜甘源吴猛刘蓓蓓武昱孜
Owner JIANGSU ACAD OF AGRI SCI
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