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Preparation of Newcastle Disease, Infectious Bronchitis and Avian Influenza Triple Inactivated Vaccine

A technology for bronchitis and Newcastle disease, applied in the field of poultry inactivated vaccine preparation, can solve the problems of virus reproduction impact, low virus titer in chicken embryo fluid, high production cost, etc., to improve vaccine stability and use effect, optimize virus culture Technology, the effect of reducing the use of chicken embryos

Active Publication Date: 2020-05-26
兆丰华生物科技(南京)有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, three parts of chicken embryos are required to produce three antigens. The amount of chicken embryos is large, the cost is high, and it takes a lot of manpower and takes a long time. The antigen before inactivation needs to be concentrated before it can be used, and the production cost is high.
[0004] 2. Part of the dual seedlings are diluted with two kinds of virus seed poisons according to a certain dilution ratio and inoculated into the same chicken embryo for cultivation at the same time, and the chicken embryo liquid is collected. The chicken embryo liquid contains two antigens, so although the amount of eggs used is reduced by half, However, because the two viruses are temporarily mixed and reproduced in the same chicken embryo, they will interfere with each other, and the virus reproduction will be affected. It can only be used after concentration, and the production cost is also high
[0005] 3. Emulsification: directly mix the inactivated antigen water phase and oil phase in a certain ratio for emulsification, no measures are taken to control the pH of the water phase, the temperature of the water phase and the oil phase, and the temperature of the vaccine during the emulsification process. The finished product is placed for 2- It is unstable at 8°C, and stratification will occur when the time is long, and the storage period is short

Method used

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  • Preparation of Newcastle Disease, Infectious Bronchitis and Avian Influenza Triple Inactivated Vaccine
  • Preparation of Newcastle Disease, Infectious Bronchitis and Avian Influenza Triple Inactivated Vaccine
  • Preparation of Newcastle Disease, Infectious Bronchitis and Avian Influenza Triple Inactivated Vaccine

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preparation example Construction

[0025] A kind of preparation method of chicken Newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine comprises the following steps:

[0026] 1) Preparation of Newcastle disease, infectious bronchitis, and avian influenza virus coexistence seed virus:

[0027] Take chicken Newcastle disease seed virus, infectious bronchitis seed virus, avian influenza virus seed virus, among them, Newcastle disease seed virus titer ≥ 10 8.9 EID 50 / 0.1ml, infectious bronchitis seed virus titer ≥10 6.9 EID 50 / 0.1ml, avian influenza virus titer ≥ 10 8.1 EID 50 / 0.1ml;

[0028] Dilute these three seed viruses by 10000-50000 times respectively, then inoculate 10-11 day-old SPF chicken embryos with the three diluted seed viruses in equal amounts, and culture the inoculated chicken embryos at 37°C for 48-72 hours, and cultivate During the period, eggs were illuminated every 4-8 hours from the 48th hour of cultivation, and the chicken embryos that died before 48 hours we...

Embodiment 1

[0039] A kind of preparation method of chicken Newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine comprises the following steps:

[0040] 1) Preparation of Newcastle disease, infectious bronchitis, and avian influenza virus coexistence seed virus:

[0041] Take chicken Newcastle disease seed virus, infectious bronchitis seed virus, avian influenza virus seed virus, among them, Newcastle disease seed virus titer ≥ 10 8.9 EID 50 / 0.1ml, infectious bronchitis seed virus titer ≥10 6.9 EID 50 / 0.1ml, avian influenza virus titer ≥ 10 8.1 EID 50 / 0.1ml;

[0042] Dilute chicken Newcastle disease seed virus by 10,000 times, infectious bronchitis seed virus by 30,000 times, and avian influenza virus seed virus by 50,000 times, and then inoculate 10-11-day-old SPF chicken embryos with equal amounts of these three diluted seed viruses , culture the inoculated chicken embryos at 37°C for 72 hours. During the cultivation period, start to cultivate eggs every...

Embodiment 2

[0061] A kind of preparation method of chicken Newcastle disease, infectious bronchitis, bird flu triple inactivated vaccine comprises the following steps:

[0062] 1) Preparation of Newcastle disease, infectious bronchitis, and avian influenza virus coexistence seed virus:

[0063] Take chicken Newcastle disease seed virus, infectious bronchitis seed virus, avian influenza virus seed virus, among them, Newcastle disease seed virus titer ≥ 10 8.9 EID 50 / 0.1ml, infectious bronchitis seed virus titer ≥10 6.9 EID 50 / 0.1ml, avian influenza virus titer ≥ 10 8.1 EID 50 / 0.1ml;

[0064] Dilute chicken Newcastle disease seed virus by 30,000 times, infectious bronchitis seed virus by 50,000 times, and avian influenza virus seed virus by 10,000 times, and then mix and inoculate 10-11-day-old SPF chicken embryos with these three diluted seed viruses in equal amounts. The inoculated chicken embryos were cultured at 37°C for 60 hours. During the cultivation period, the eggs were il...

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Abstract

The invention relates to a preparation method of a triple inactivated vaccine for a Newcastle disease and infectious bronchitis as well as avian flu. The preparation method of the triple inactivated vaccine for the Newcastle disease and the infectious bronchitis as well as the avian flu comprises the following steps of (1), preparing a coexisting seed virus for the Newcastle disease and the infectious bronchitis as well as the avian flu; (2), preparing an antigen; (3), centrifuging the antigen; (4), inactivating the antigen; (5), carrying out emulsification, so as to finally obtain the finished product triple vaccine. According to the preparation method of the triple inactivated vaccine for the Newcastle disease and the infectious bronchitis as well as the avian flu, through optimizing a virus culturing process, the usage amount of chick embryos is decreased; the production cost is reduced; the labor efficiency is improved; the titer of the antigen for three viruses is improved; the concentration is needed for the finished product antigen; the stability and the usage effect of the vaccine are improved.

Description

technical field [0001] The invention relates to a method for preparing an inactivated vaccine for poultry, in particular to a method for preparing a triple inactivated vaccine for Newcastle disease, infectious bronchitis and avian influenza. Background technique [0002] The preparation method of the existing Newcastle disease, infectious bronchitis and avian influenza triple inactivated vaccine has the following steps: the preparation of the three virus antigens of Newcastle disease, infectious bronchitis and avian influenza, the concentration and inactivation of the antigen, and the emulsification of the finished product . The existing method mainly has the following defects: [0003] 1. The preparation method of the three antigens is to use the Newcastle disease, infectious bronchitis, and avian influenza three kinds of viruses to dilute them according to a certain dilution factor and inoculate them into chicken embryos respectively, place them in 37°C for a certain peri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/17A61K39/145A61K39/215A61P31/14A61P31/16C12N7/00
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70C12N7/00C12N2760/16134C12N2760/18134C12N2770/20034A61K2300/00
Inventor 江兴华陈静怡陈丽萍包松英
Owner 兆丰华生物科技(南京)有限公司
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