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Application of bamboo ginseng polysaccharide in preparation of drugs for preventing and/or treating liver cancer

A technology of Panax ginseng polysaccharide and liver cancer, which is applied in the directions of drug combination, pharmaceutical formula, antitumor drug, etc.

Active Publication Date: 2021-05-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Have not yet seen related reports in the prior art, this application will fill up this gap

Method used

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  • Application of bamboo ginseng polysaccharide in preparation of drugs for preventing and/or treating liver cancer
  • Application of bamboo ginseng polysaccharide in preparation of drugs for preventing and/or treating liver cancer
  • Application of bamboo ginseng polysaccharide in preparation of drugs for preventing and/or treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of bamboo ginseng polysaccharide:

[0018] The raw material of bamboo ginseng is the dried rhizome of Panax japonicus C.A.Mey., a plant of the Araliaceae genus Panax japonicus.

[0019] Proceed as follows:

[0020] Take the dried bamboo ginseng medicinal powder, add 95% ethanol (V / V, the percentage concentration of ethanol mentioned in this manual refers to the volume percentage concentration, and will not be described later), reflux at 60 ° C for 3 hours, and degrease;

[0021] Then add 80% ethanol, reflux at 60°C for 2 hours, remove monosaccharides and oligosaccharides;

[0022] After drying the dregs in an oven, use purified water as a solvent to reflux extract 3 times, 2 hours each time, combine the filtrate and concentrate it in a rotary evaporator until it is in the state of liquid extract, add ethanol, and adjust the ethanol concentration to 80 %, stay overnight at 4°C, filter the next day, precipitate and dry to obtain the crude bamboo ginseng;

...

Embodiment 2

[0032] Study on anti-H22 liver cancer cell activity and general toxicity of the bamboo ginseng polysaccharide prepared in Example 1

[0033] 2.1 Experimental materials

[0034] 2.1.1 Animals and cell lines

[0035] Kunming mice: half male and half male, weighing 18-22 g, provided by the Hubei Provincial Center for Disease Control and Prevention, license number: SCXK (Hubei): 2008-0003.

[0036] The H22 cell line was donated by Mr. Chen Luyi, School of Pharmacy, South Central University for Nationalities, and stored in a -80°C refrigerator. Centrifuge during recovery, remove DMSO in the cryopreservation solution, dilute with normal saline to 1*10 6 1 / mL, inoculate 0.2mL of each mouse in the abdominal cavity, pass passage after seven days, take the ascites of the third-generation H22 ascites tumor mouse, dilute it with normal saline at 1:10, and inoculate 0.2mL of each mouse on the right forelimb of the mouse Under the skin of the axilla, the H22 solid tumor model was establi...

Embodiment 3

[0071] Protective effect of the bamboo ginseng polysaccharide prepared in Example 1 on LPS / D-GalN-induced acute liver injury in mice:

[0072] LPS / D-GalN induced acute liver injury in mice was used as a model to study the preventive and protective effects of bamboo ginseng polysaccharides on acute liver injury and try to explore its mechanism.

[0073] 3.1 Experimental animals and reagents

[0074] 3.1.1 Experimental animals

[0075] 48 male Kunming mice of SPF grade, weighing 18-22 g, were purchased from Hubei Experimental Animal Research Center, experimental animal production license number: SCXK (Hubei) 2015-0018.

[0076] 3.1.2 Reagents

[0077] Panax ginseng polysaccharide: prepared in Example 1; lipopolysaccharide (LPS) was purchased from Biosharp; galactosamine (D-GalN) was purchased from Tokyo Chemical Industry; alanine aminotransferase (ALT), aspartic acid amino Transferase (AST), reduced glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) assay...

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Abstract

The invention belongs to the technical field of medical application of plant polysaccharides, and specifically discloses the application of bamboo ginseng polysaccharides in the preparation of drugs for preventing and / or treating liver cancer. The present application found for the first time that PSPJ (30‑100 mg / kg) had a significant inhibitory effect on the growth of tumor cells in liver cancer H22 tumor-bearing mice (the tumor inhibition rates were 41.7% and 54.3%, respectively). Moreover, PSPJ had no obvious toxic effect on the general physical condition, blood routine index, liver and kidney function of liver cancer H22 tumor-bearing mice. The present application also found that PSPJ has a clear preventive and protective effect on acute liver injury induced by LPS / D-GalN in mice, and the mechanism of action may be that PSPJ prevents and prevents liver injury by inhibiting the occurrence of oxidative stress response and inflammatory response , improve the function of the damaged liver, and finally prevent the possibility of liver damage transforming into liver cancer.

Description

technical field [0001] The invention relates to the technical field of medical application of plant polysaccharides, in particular to the application of bamboo ginseng polysaccharides in the preparation of drugs for preventing and / or treating liver cancer. Background technique [0002] Liver cancer is a kind of malignant tumor that seriously endangers human health, and its morbidity and mortality remain high. The pathogenesis of liver cancer is very complex, among which chronic persistent inflammation and loss of tumor suppressor genes are important factors leading to the occurrence of liver cancer. At present, the mechanisms of most tumor suppressor genes regulating the proliferation and apoptosis of liver cancer cells have been studied clearly. In view of the fact that some tumor suppressor genes are also expressed in immune cells, they have dual functions of inhibiting inflammation and tumor in both immune cells and tumor cells, and then by targeting the common signaling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P35/00
CPCA61K31/715
Inventor 邓旭坤江善青任永申
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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