Preparation method of modified algicide
An algicide and modification technology, which is applied in the direction of herbicides and algicides, botanical equipment and methods, biocides, etc., can solve the problems of poor persistence of algae inhibition effect, temporary cure, not root cause, and harm, and achieve algae inhibition The effect of less ingredient consumption, high ecological safety, and simple preparation process
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[0020] The preparation method of modified algicide, the method comprises the steps of:
[0021] (1) Separately dissolve a certain amount of linoleic acid and artemisinin;
[0022] (2) Prepare N groups of linoleic acid artemisinin solutions with different ratios;
[0023] (3) Add an emulsifier to the linoleic acid artemisinin solution with different proportions in group N for emulsification;
[0024] (4) After emulsification, add sodium alginate and mix evenly to form a mixed solution of group N;
[0025] (5) Drop the above mixed solution into the CaCl2 solution to form a modified algicide gel;
[0026] (6) Rinse the modified algicide gel with distilled water, and dry to obtain the modified algicide.
[0027] The step (1) dissolves a certain mass of linoleic acid and artemisinin respectively, the specific method is: use two parts of ethyl oleate with a certain mass percentage concentration of 2% to 5% to dissolve linoleic acid and artemisinin respectively element, and respe...
Embodiment 1
[0036] The preparation method of the modified algicide, the preparation steps are as follows:
[0037] (1) Dissolve 0.6g linoleic acid and 0.6g artemisinin in two portions of 3mL ethyl oleate with a concentration of 100% by mass, and add 2mL methanol as a co-solvent to each, at an ultrasonic frequency of 40KHz and a temperature of 25°C Sonicate for 15 minutes under conditions to accelerate dissolution;
[0038] (2) Mix the dissolved linoleic acid and artemisinin in proportion to form three sets of ratio systems, the ratios are linoleic acid:artemisinin=1:4, 1:1, 4:1;
[0039] (3) Add 6 mL of TWEEN-20 with a mass percentage concentration of 100% to the above three groups of systems for emulsification. The emulsification time is 25 minutes, and the emulsification temperature is 48 ° C. After emulsification, use distilled water to prepare 50 mL of the three groups of solutions respectively;
[0040] (4) Add 1g of sodium alginate with a mass percentage concentration of 100% to th...
Embodiment 2
[0046] The preparation method of the modified algicide, the preparation steps are the same as in Example 1.
[0047] Use above-mentioned modified algicide to carry out acute toxicity experiment to the cultivated Microcystis aeruginosa, the added concentration of described modified algicide is 1g / L, and the initial concentration of described Microcystis aeruginosa is 2×10 5cell / L, which can simulate the water body in the state of cyanobacteria not blooming, set up three parallel groups, one control group (without adding modified algicide), observe once every 24h, and observe continuously for 10 days, through the attached figure 2 It can be seen that for the modified algicide with linoleic acid: artemisinin=4:1 ratio, the inhibition rate of Microcystis aeruginosa reaches 87%, and the continuous observation for 30 days, for linoleic acid: artemisinin=4 :1 ratio of the modified algicide, the inhibition rate of Microcystis aeruginosa reached 92.5%, and the algae inhibition effect ...
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