Method for preparing microfluidic channels in glass by simple hot-pressing process

Abstract

The invention discloses a method for preparing microfluidic channels in glass by a simple hot-pressing process. The method includes: designing a microfluidic channel pattern by AUTOCAD (auto computeraided design), and grinding and polishing glass to make surface roughness of the glass not larger than 50micron; inscribing the pattern on a nickel alloy substrate by laser direct writing, subjectinga normal glass slide to hot pressing treatment by a normal electric furnace and a pressure plate, aligning the nickel alloy substrate to the glass, putting the pressure plate, setting an appropriate heat procedure, and finally separating the nickel alloy substrate at the room temperature to finish glass based microfluidic channel preparation. Preparing the microfluidic channels 150micron in depthonly takes about 2.2h, low manufacturing cost and short period are realized, the process is simple, inner walls of the microfluidic channels are smooth and free of extra modification, and easiness inpopularization and utilization is achieved. The method has advantages that simplicity, feasibility, nontoxicity, freeness of pollution, environmental friendliness and easiness in technical mastering are realized, reaction is performed under conditions of the set heat procedure and pressure, and batch production can be realized.

Description

technical field [0001] The invention relates to the technical field of preparation of microfluidic biochip microchannels, in particular to a preparation method of glass-based microchannels. Background technique [0002] Microfluidic chip has the characteristics of less sample consumption, small volume and fast analysis speed, and can realize high automation and integration. A dynamic instrumental approach platform. The main applications focus on large-scale, high-throughput, low-cost life science and analytical chemistry experiments, including single cell culture and analysis, stem cell manipulation and culture, single molecule biophysics, high-throughput cell and molecular biology screening Experimentation, drug discovery, high-throughput synthetic biology, high-throughput sequencing technology, single-cell genomics, etc. [0003] At present, the materials used to make chips are glass and polymers. The biggest disadvantages of high molecular polymers including PDMS, PMMA...

AI Technical Summary

Problems solved by technology

However, the preparation of microchannels on glass surfaces requires expensive and complex photolithography techniques.

[0005] The existing techniques for preparing glass-based microfluidic channels are photolithography and acid etching, but this process first needs to process a sacrificial layer with microchannel patterns on the surface of the substrate by photolithography. The material of the sacrificial layer is generally chromium, gold, Molybdenum and other metals and photoresist, sacrificial layer patterns are generally processed by photolithography, and the steps involved in mask making, exposure and development require expensive instruments, ultra-clean room environment and cumbersome preparation process, so it cannot meet the requirements of rapid and rapid production. The need for mass production; secondly, traditional acid etching is often achieved by immersing the substrate in a large amount of etching solution system combined with mechanical stirring, so methods are often used to protect the back of the substrate and other areas that do not need to be processed before etching. , very cumbersome; in addition, these etching solutions used for microchannel preparation are mostly strong acids, strong alkalis or toxic organic solvents, which are easy to pollute the environment, waste liquid treatment is very cumbersome, and the cost is high

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