A zwitterionic magnetic composite hydrogel immobilized enzyme carrier and its preparation method

Abstract

The invention relates to a zwitterionic magnetic composite hydrogel immobilized enzyme carrier and a preparation method thereof; the carrier of the immobilized enzyme is a zwitterionic hydrogel embedded with iron ferric oxide nanoparticles; One end of the aldehyde is linked to the zwitterionic hydrogel, and the other end forms a Schiff base with the amino group on the surface of the enzyme, covalently linking the enzyme to the surface of the hydrogel carrier. Mix the zwitterionic monomer, crosslinking agent, initiator and solvent to form a mixed solution, and mix it with the ferric oxide solution at a volume ratio of 3:5 to 3:10; the mixed solution containing ferric oxide Ultraviolet irradiation or heating or placing at normal temperature enables the monomer to embed ferric oxide through cross-linking polymerization to form a zwitterionic magnetic composite hydrogel immobilized enzyme carrier embedded with ferric oxide. The enzyme immobilization method provided by the invention inherits the advantages of the traditional covalent binding method for immobilizing enzymes, has a high recovery rate of enzyme activity, and has high application value.

Description

technical field [0001] The invention relates to a kind of zwitterionic magnetic composite hydrogel immobilized enzyme carrier embedded with ferric oxide. The invention relates to the preparation method and application of the carrier. More specifically, the present invention relates to a preparation method of a zwitterionic magnetic composite hydrogel immobilized enzyme carrier embedded with ferric oxide and the use of the carrier immobilized enzyme prepared by the method. Background technique [0002] Enzymes are an important class of biocatalysts, which can participate in various metabolic reactions in organisms, and have the advantages of mild action conditions, high efficiency, specificity, and wide sources. Enzymes are widely used in brewing, medicine, food processing, chemical analysis, environmental protection, energy development and other fields. However, most natural enzymes are proteins, and the high-level structure of protein enzymes is very sensitive to the envi...

AI Technical Summary

Problems solved by technology

[0004] Enzyme immobilization carriers have been widely used from the initial natural polymer materials to later synthetic polymer materials, inorganic materials, and now composite materials, but still have more or less defects: 1) Chitosan, sodium alginate, gelatin, carrageenan, etc. are widely used in natural polymer materials.

The biocompatibility of synthetic polymer materials is not high, such as acrylamide monomer is neurotoxic to organisms

3) The structure of the inorganic carrier material is not easy to control, which affects mass transfer, and the ability to bind enzymes is poor, which has a greater impact on enzyme activity

The disadvantage is that the magnetic material has poor biocompatibility and cannot provide a good hydrophilic environment for the protein, which is not conducive to the biological activity of the enzyme; and the magnetic material cannot resist protein adsorption. When used to immobilize the enzyme carrier 2) The hydrophilic carrier has good biocompatibility and can improve the affinity of the substrate for the enzyme, which is beneficial for the enzyme Promoting reactions create a favorable microenvironment

But so far no one has applied it to the field of enzyme immobilization

Moreover, simply using zwitterionic materials as enzyme immobilization carriers is not easy to separate from the reaction system and does not have reproducibility

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