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Method used for recycling microalgae residue and producing spirulina rich in polysaccharides

A technology of spirulina and microalgae, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable scale expansion, high corrosion resistance requirements, etc., achieve good sustainability, improve utilization efficiency, The effect of reducing sewage discharge

Inactive Publication Date: 2017-05-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The temperature required for acid-heat hydrolysis liquefaction is 100-110°C, and reflux under normal pressure is sufficient, but hydrochloric acid, sulfuric acid or organic acids need to be added, which requires high corrosion resistance of equipment and is not suitable for scale-up

Method used

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  • Method used for recycling microalgae residue and producing spirulina rich in polysaccharides
  • Method used for recycling microalgae residue and producing spirulina rich in polysaccharides

Examples

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Effect test

Embodiment 1

[0027] Spirulina with high protein and low carbohydrate content (protein content 42%, carbohydrate content 16%) is used as algae species, and hydrothermal carbonation liquid is used as nitrogen source and part of carbon source to cultivate spirulina under nitrogen-limited conditions, and the production of spirulina rich in polysaccharide biomass.

[0028] 1) In a 2L stainless steel autoclave, add 1.8L deionized water, and slowly add 180 g of high-sugar spirulina (carbohydrate content 44%) algae residue 180 g into the reactor after extracting 4 hours in hot water at 80 ° C to obtain a solid Uniform algae pulp with a liquid mass ratio of 1:10. The reactor was sealed and purged with nitrogen for 2 min to maintain an inert atmosphere. Heat the reactor to 200°C at a rate of 1.5°C / min, and keep the temperature constant for 3h. Cool the reactor to room temperature at a rate of 3 °C / min with cooling circulating water. Open the purge valve to release residual gas, filter the reactio...

Embodiment 2

[0033] Spirulina with low protein and high carbohydrate content (protein content 22%, carbohydrate content 53%) is used as algae species, and hydrothermal carbonation liquid is used as nitrogen source and part of carbon source to cultivate spirulina under nitrogen-limited conditions, and the production of spirulina rich in Polysaccharide biomass, while removing most of the ammonia nitrogen and part of the organic nitrogen in the medium.

[0034] 1) The process of hydrothermal carbonization recovery of nutrients in the algae residue is the same as in Example 1.

[0035] 2) Spirulina algae Spirulina algae cells are cultured in nutrient-stressed inorganic salt medium (MSM) to control the initial NaNO 3 The concentration is 85mg / L, the initial inoculation density OD 560 0.3, culture temperature 26~30℃, artificial light-to-dark ratio 14h:10h, light intensity on the light-receiving surface of the culture medium 40μmol / (m 2 s), after 6 days of culture, the algal cells were in a sta...

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Abstract

The invention relates to a method used for recycling microalgae residue and producing spirulina rich in polysaccharides. According to the method, hydrothermal carbonization is adopted to recycle nutrients in microalgae residue as nitrogen sources, carbon sources, and other nutrient elements in culturing of spirulina, conventional nutritive salt (such as sodium nitrate and urea) supplying method is replaced to produce spirulina rich in polysaccharides, the content of polysaccharide in biomass is 65% or higher, and is increased by 15 to 56% compared with that of common culture medium; polysaccharide yield is increased by 23% to 3.5 times of that of common culture medium. The method is capable of realizing resource utilization of wastes, reducing consumption of nutrient salts, increasing spirulina polysaccharide yield and quality, and reducing production cost, and is beneficial for simplifying of downstream processing operation, and promoting of industrialized production of spirulina.

Description

technical field [0001] The present invention relates to the recovery and utilization of microalgae dregs and the cultivation of microalgae. Specifically, the nutrients such as nitrogen source and carbon source in the microalgae dregs are recovered by hydrothermal treatment and used for the production of rich-rich spirulina through appropriate dilution. polysaccharide biomass. It can be used in waste resource utilization, waste water treatment, spirulina cultivation and production of biomass, as well as food, medicine, energy and other related fields using spirulina polysaccharide as raw material. Background technique [0002] Spirulina polysaccharide is a water-soluble polysaccharide in Spirulina cells. It has biological activities such as anti-tumor, anti-radiation, and anti-mutation. It can improve the immune function of body cells and body fluids, resist the proliferation of cancer cells, and reduce the genetic damage caused by radiation. It has broad application prospec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 薛松姚长洪刘瑞国陆洪斌潘彦斐吴佩春曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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