Method for analyzing adrenocortical hormone fluorometholone content and related substances
A technique for adrenal cortex and related substances, which is applied in the directions of analyzing materials, measuring devices, material separation, etc., can solve the problems of poor chromatographic peak shape, large flow rate, and high column pressure, and achieves simple and convenient operation process, accurate test results, Highly feasible effect
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Embodiment 1
[0043] 1. Chromatographic conditions:
[0044] High performance liquid chromatography: Agilent 1260HPLC
[0045] Chromatographic column: ZORBAX SB-C18, 250*4.6mm, 5μm
[0046] Detector: UV detector Detection wavelength: 235nm
[0047] Injection volume: 10μl Column temperature: 35℃
[0048] Flow rate: 1.0ml / min
[0049] Analytical balance: ten thousandth
[0050] Mobile Phase: A: Water B: Acetonitrile
[0051] 2. The gradient conditions are as follows:
[0052]
[0053] 3. Test results: as shown in Table 1 below.
[0054] Table 1
[0055]
[0056]
[0057] The separation between fluorometholone and the front and back impurities is greater than 2.0, and the separation between other peaks is greater than 1.5;
[0058] figure 1 It is the liquid phase spectrogram of embodiment 1 of the present invention.
Embodiment 2
[0060] 1. Chromatographic conditions:
[0061] High performance liquid chromatography: Agilent 1260HPLC
[0062] Chromatographic column: ZORBAX SB-C18, 250*4.6mm, 5μm
[0063] Detector: UV detector Detection wavelength: 254nm
[0064] Injection volume: 10μl Column temperature: 30℃
[0065] Flow rate: 1.2ml / min
[0066] Analytical balance: ten thousandth
[0067] Mobile Phase: A: Water B: Acetonitrile
[0068] 2. The gradient conditions are the same as those in Example 1.
[0069] 3. Test results: as shown in Table 2 below.
[0070] Table 2
[0071]
[0072]
[0073] The separation between fluorometholone and impurities after the main peak is less than 2.0, and the separation between other peaks is greater than 1.5.
Embodiment 3
[0075] 1. Chromatographic conditions:
[0076] High performance liquid chromatography: Agilent 1260HPLC
[0077] Chromatographic column: ZORBAX SB-C18, 250*4.6mm, 5μm
[0078] Detector: UV detector Detection wavelength: 254nm
[0079] Injection volume: 10μl Column temperature: 25℃
[0080] Flow rate: 2.0ml / min
[0081] Analytical balance: ten thousandth
[0082] Mobile Phase: A: Water B: Acetonitrile
[0083] 2. The gradient conditions are the same as those in Example 1.
[0084] 3. Test results: The relevant data are listed in Table 3 below.
[0085] table 3
[0086]
[0087]
[0088] The separation between fluorometholone and impurities after the main peak is less than 2.0, and the separation between other peaks is greater than 1.5.
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