A kind of myricetin extract, the pharmaceutical composition containing it and its use

A technology of extract and myricetin, which is applied in the pharmaceutical field, can solve unseen problems, achieve the effect of relieving side effects, ensuring biological activity, and broad clinical application prospects

Active Publication Date: 2021-01-12
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been found that myricetin also has an anti-tumor effect, but so far there has been no report of using myricetin as a drug to inhibit tumor metastasis

Method used

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  • A kind of myricetin extract, the pharmaceutical composition containing it and its use
  • A kind of myricetin extract, the pharmaceutical composition containing it and its use
  • A kind of myricetin extract, the pharmaceutical composition containing it and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: the preparation of myricetin extract of the present invention

[0075] 1. Raw materials and equipment

[0076] Black beans (produced in Heilongjiang), ethanol, petroleum ether, methanol, chloroform, silica gel;

[0077] Vacuum drying oven, centrifuge, mortar, sieving apparatus, microwave ultrasonic instrument, rotary evaporator, vacuum pump, vacuum freeze drying oven;

[0078] 2. Extraction method (take black soybean skin as an example)

[0079] 2.1. Soak and peel the black beans, wash, dry, and then crush and sieve to obtain 80-100 mesh black soybean powder. Accurately weigh 10g of black soybean skin powder, dissolve the black soybean skin powder in 150mL of petroleum ether, and reflux at 80°C to degrease 2 hours; then centrifuge at 5000rpm, 25°C for 10min, remove the supernatant, vacuum dry and pulverize to 80-100 mesh at 60°C, vacuum degree -0.07MPa;

[0080] 2.2. Dry the degreased black soybean hulls at low temperature, dissolve them in 90 mL of m...

Embodiment 2

[0088] Embodiment 2: the preparation of myricetin extract of the present invention

[0089] 1. Reagents and raw materials

[0090] Bayberry leaves (produced in Zhejiang), ethanol, petroleum ether, methanol, chloroform, silica gel; vacuum oven, centrifuge, mortar, sieve, microwave ultrasonic instrument, rotary evaporator, vacuum pump, vacuum freeze-drying box;

[0091] 2. Extraction method (taking bayberry leaves as an example)

[0092] 2.1. Crush and sieve red bayberry leaves to obtain 80-100 mesh red bayberry leaf powder. Accurately weigh 10g of red bayberry leaf powder, dissolve the red bayberry leaf powder in 150mL petroleum ether, and reflux and degrease at 80°C for 2 hours; then at 5000rpm, Centrifuge at 25°C for 10 minutes, remove the supernatant, vacuum-dry and pulverize to 80-100 mesh at 60°C and vacuum degree of -0.07MPa;

[0093] 2.2. Dry the degreased red bayberry leaves at low temperature, dissolve them in 90mL of 75% methanol aqueous solution by volume, and e...

Embodiment 3

[0101] Embodiment 3: the in vitro growth inhibition test of myricetin extract to human breast cancer brain metastases cell line (BCBM), transfection migration inhibition assay and invasion inhibition assay

[0102] 1. Materials and methods

[0103] 1.1. Reagents and raw materials

[0104] 6-well plate, Transwell chamber (pore size 8 μm), 1640 basal medium, 1640 complete medium (containing 10% fetal bovine serum), 0.25% trypsin, penicillin-streptomycin, Giemsa's Stain, MTT (5mg / mL); Human breast cancer brain metastasis cell line (BCBM).

[0105] 2. Method

[0106] 2.1 In vitro growth inhibition test (MTT method) of myricetin extract on human breast cancer brain metastases cell lines

[0107] Collect logarithmic phase BCBM cells, adjust the concentration of cell suspension to 8×10 4 Cells / mL, add 100 μL per well to plate the cells to be tested at a density of 8000 cells / well and inoculate them in a 96-well plate. 5%CO 2 , incubate at 37°C until the cells adhere to the ...

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Abstract

A myricetin extract, prepared by an extraction method comprising the following steps: (1) plants of Myricaceae, Grape, Leguminosae or Camelliaceae are crushed, degreased and then dissolved in a methanol aqueous solution, and extracted by microwave and ultra-sonication to obtain an extract solution; (2) the extract solution obtained in step (1) is concentrated under a reduced pressure to obtain a concentrated solution, which is then filtrated under a reduced pressure and extracted with trichloromethane, and the resulting extract solution is collected, freeze-dried and pulverized; (3) a powder obtained in step (2) is dissolved in petroleum ether and then purified by means of silica gel column, so as to collect an eluate containing myricetin, which is then concentrated to obtain a crude product of myricetin; (4) the crude product of myricetin obtained in step (3) is added to a methanol aqueous solution having a volume ratio of 50-75%, and is heated to 75-80ºC till completely dissolved and then stirred, the extract is obtained following cooling and standing, crystallizing, filtrating and drying. The myricetin extract is used to prevent or treat tumor metastasis.

Description

technical field [0001] The invention belongs to the technical field of pharmacy. Specifically, the present invention relates to the myricetin extract extracted from plants, the pharmaceutical composition containing it and its preparation for preventing and / or treating diseases caused by tumor metastasis or for inhibiting tumor cell migration. use in . Background technique [0002] According to the latest report of the International Cancer Research Center (IARC), cancer patients worldwide are increasing at an average annual rate of 3%-5%, and cancer has become the largest public health problem in the world. According to the report of the World Health Organization, about 80% of cancer patients worldwide died of recurrence and metastasis after surgery, radiotherapy and chemotherapy. According to data from the American Cancer Society in 2015, the overall 5-year survival rate of patients after tumor metastasis is much lower than that of primary cancer. Survival rate. The terrib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61K36/87A61K36/48A61K36/82A61P35/00A61P35/04A23L33/105
CPCA23V2002/00A61K36/185A61K36/48A61K36/82A61K36/87A61K2236/333A61K2236/51A61K2236/53A61K2300/00A23V2200/308A23V2250/21A23V2250/214A23V2300/14A23V2300/48A23V2300/50A23L33/105A61P35/00A61P35/04
Inventor 韩梅慈英倩
Owner BEIJING NORMAL UNIVERSITY
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