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Method for detecting microcystin LR based on fluorescence resonance energy transfer of shell-core type up-conversion material and molybdenum disulfide

A fluorescence resonance energy and microcystin technology, applied in the field of nanomaterials and analytical chemistry, can solve the problems of narrow linear range, low sensitivity, complicated operation, etc., and achieve simplified analysis and detection process, short detection time, improved stability and specific effect

Inactive Publication Date: 2017-04-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, among the traditional methods for detecting MC-LR in water, high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry have better detection results, but the equipment is expensive, the operation is cumbersome, and the operator has high technical requirements ; Plant cell bioassays and protein phosphatase inhibition are relatively less sensitive; ELISA is more sensitive but has a narrow linear range

Method used

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  • Method for detecting microcystin LR based on fluorescence resonance energy transfer of shell-core type up-conversion material and molybdenum disulfide
  • Method for detecting microcystin LR based on fluorescence resonance energy transfer of shell-core type up-conversion material and molybdenum disulfide
  • Method for detecting microcystin LR based on fluorescence resonance energy transfer of shell-core type up-conversion material and molybdenum disulfide

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Embodiment 1

[0025] Example 1: Establishment of standard curve for detection of microcystin LR in actual samples of tap water and Taihu Lake water and pretreatment of test samples: The collected tap water and Taihu Lake water samples were pretreated by centrifugation, filtration, and membrane passage in sequence. The clear liquid was collected for later use. The content of microcystin LR was determined by the method of the present invention and high performance liquid chromatography, and the results are shown in Table 1. The detection results of the two methods were consistent with no significant difference.

[0026] Table 1: actual sample detection, method of the present invention and HPLC method contrast

[0027]

[0028] Note: ND is not detected

Embodiment 2

[0029] Example 2: Detection of microcystin LR in actual water samples and the experimental sample pretreatment of standard addition recovery rate is the same as that of Example 1.

[0030] Taking the 6 groups of microcystin LR concentration data obtained in Example 1 as the background value, three different concentrations of microcystin LR standard substances were added thereto, and the method of the present invention was used to detect again the microcystin LR content to obtain the detection value. Recovery %=(detection value-background value) / addition amount×100%.

[0031] It can be seen from the data in Table 2 that the recovery rate is between 94% and 112%, indicating that the present invention is stable, sensitive and accurate, and is applicable to the detection of microcystin LR in actual water samples.

[0032] Table 2: Detection and recovery of microcystin LR in actual samples

[0033]

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Abstract

A method for detecting microcystin LR based on the fluorescence resonance energy transfer of a shell-core type up-conversion material and molybdenum disulfide is used for detecting the microcystin LR (MC-LR) in a practical water sample. The shell-core type up-conversion material (NaYF4:Yb, Tm@NaYF4:Yb) is prepared to improve the fluorescence intensity. After the up-conversion material is connected with the nucleic acid adaptor of the MC-LR, nucleic acid bases is adsorbed to the bottom surface of the molybdenum disulfide through Van der Waals force, the fluorescence resonance energy transfer occurs between the up-conversion material and molybdenum disulfide, and fluorescence is quenched. When the MC-LR exists in the detection process, the MC-LR specifically binds to the adaptor of the MC-LR, the conformation of the adaptor is changed, and the adaptor is separated from the bottom surface of the molybdenum disulfide to recover the fluorescence. The fluorescence intensity at 361 nm is monitored, the MC-LR is quantitatively detected, the linear range is 0.01-50 ng / ml, and the detection limit is 0.002 ng / ml. The method used for detecting the MC-LR has the advantages of high sensitivity, high specificity and good stability. A result is accurate and reliable when the method is used for detecting tap water and Taihu Lake water.

Description

technical field [0001] The invention discloses a method for detecting microcystin LR based on fluorescence resonance energy transfer of a core-shell type upconversion material and molybdenum disulfide, which relates to the technical field of nanomaterials and analytical chemistry, and is used for detecting microcystin LR in water. Background technique [0002] Microcystins (MCs) are a class of highly toxic and acutely harmful cyclic heptapeptide hepatotoxins produced by freshwater blue-green algae, which have serious impacts on aquatic organisms, human drinking water safety and human health. Among them, Microcystin-(leucine-arginine), MC-LR is one of the most common and acutely toxic microcystins. It has strong liver toxicity, and also has certain toxicity to the heart, kidney, spleen and gastrointestinal tract. At present, among the traditional methods for detecting MC-LR in water, high-performance liquid chromatography and high-performance liquid chromatography-mass spect...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/58
CPCG01N21/6428G01N33/582G01N2021/6432
Inventor 王周平吕佳佳吴世嘉段诺夏雨马小媛
Owner JIANGNAN UNIV
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