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Door control type medicine composition integrating cancer imaging and phototherapy and preparation method

A drug compound and imaging technology, which is applied in the field of biomedicine and achieves the effects of simple preparation process, low price and easy availability of raw materials

Inactive Publication Date: 2017-02-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no co-loading of indocyanine green ICG and phase change material 1-carbon alcohol TD on CuS@mSiO 2 Core-shell structure forms a gated drug complex integrating cancer imaging and phototherapy (CuS@mSiO 2 -TD / ICG) reports

Method used

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  • Door control type medicine composition integrating cancer imaging and phototherapy and preparation method
  • Door control type medicine composition integrating cancer imaging and phototherapy and preparation method
  • Door control type medicine composition integrating cancer imaging and phototherapy and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for preparing a gated drug complex integrating cancer imaging and phototherapy, comprising the following steps:

[0042] (1) Core-shell structure CuS@mSiO 2 Preparation of nanoparticles:

[0043] A. The proportion, the 13mgCuCl 2 and 24 mg Na 2 S·9H 2 O was added to 100 mL of 0.13 mg / mL cetyltrimethylammonium bromide (CTAB) aqueous solution to obtain a brown solution, stirred at room temperature for 18 minutes, and heated at 90°C under nitrogen protection to make the color change. Turn into dark green, continue to react for 60 minutes, cool to 70°C, then add cetyltrimethylammonium bromide to make the total concentration of cetyltrimethylammonium bromide 2mg / mL, stir for 16 minutes, Adjust the pH to 10, add 400 μL tetraethyl orthosilicate (TEOS) and 2 mL ethyl acetate, react for 28 minutes, add 130 μL silane-modified PEG with a weight average molecular weight of 700-3000, react for 130 minutes, centrifuge, and precipitate with absolute ethanol wash 3 times; ...

Embodiment 2

[0053] Temperature-mediated ICG release experiments:

[0054] In this experiment, an external water bath was used to adjust the required temperature. First, 5 mg of CuS@mSiO prepared in Example 1 2 -TD / ICG was dispersed into 2 mL of ultrapure water, and the dispersion was placed in a dialysis bag, and the whole was placed in a phosphate buffered saline release medium with a pH of 7.4. We choose two temperature modes: constant temperature mode and beating variable temperature mode. In the constant temperature mode, we compared CuS@mSiO at room temperature (25°C), 37°C and 45°C 2 - Release amount of ICG in TD / ICG; In the variable temperature mode, choose 37°C and 45°C alternately as the heat source to observe the drug release behavior.

[0055] Such as image 3 As shown in a, at room temperature and 37 °C, the release amount of ICG is very small, because the temperature at this time is lower than the phase transition temperature of TD, it is in a solid state, and ICG cannot ...

Embodiment 3

[0057] Test CuS@mSiO 2 -TD / ICG photothermal heating curve:

[0058] Take 1ml of CuS@mSiO 2 -TD / ICG aqueous solution (CuS@mSiO 2 The concentration is 200μg / mL, while the ICG concentration is 10μg / mL) into a 1.5mL EP tube, use 808nm, 1.5W / cm 2 The power laser was irradiated for 5 minutes, and the temperature change within 0-5 minutes was recorded using a temperature monitor equipped with a thermocouple microprobe (φ=0.5mm). Such as Figure 4 Shown is CuS@mSiO 2 -The photothermal heating curve of TD / ICG, it can be seen that the prescription can rapidly raise the temperature under light to achieve the purpose of photothermal therapy.

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Abstract

The invention discloses a door control type medicine composition integrating cancer imaging and phototherapy and a preparation method. The preparation method comprises the following steps: (1) preparing CuS@mSiO2 nano particles in a core-shell structure; (2) preparing ammoniated CuS@mSiO2 nano particles; and (3) preparing CuS@mSiO2-TD / ICG. Each CuS@mSiO2 nano particle in the core-shell structure, prepared in the invention, is provided with a silicon dioxide shell with a suitable thickness, so that the common load of indocyanine green and TD can be guaranteed; the CuS@mSiO2-TD / ICG can well play a preferable door control role, and the release of indocyanine green is controlled; and during systemic circulation, the effect of opto-thermodynamics and photodynamics combination therapy is achieved. The door control type medicine composition has good water solubility and biocompatibility. The therapeutic effect of tumors can be effectively strengthened, and the toxic and side effects are reduced. The preparation technology is simple, the method is stable and reliable, the reaction controllability is high, and the used raw materials are easily obtained and are low in cost.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesoporous-gated drug compound integrating cancer imaging and phototherapy that is remotely regulated by near-infrared light, a preparation method and an application. Background technique [0002] Near-infrared light-induced photothermal therapy is an emerging non-invasive minimally invasive treatment technology for tumors in recent years. It mainly uses photothermal conversion agents to convert near-infrared light energy into local heat energy to kill cancer cells, thereby greatly reducing the damage to normal tissues. Cell toxicity, so it has broad application prospects in cancer treatment. As an emerging nanomaterial, copper sulfide has attracted extensive attention due to its strong near-infrared absorption and high photothermal conversion efficiency. However, many recent reports have pointed out that high-concentration copper sulfide nanoparticles have poor biocompa...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K33/34A61K49/00A61K49/22A61K9/14A61K47/04A61P35/00
CPCA61K41/0052A61K9/143A61K33/34A61K41/0042A61K41/0057A61K49/0017A61K49/225
Inventor 李楠游青孙琪王金平谭潇潇庞晓娟刘丽
Owner TIANJIN UNIV
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